Soybean seeds contain many biologically active secondary metabolites, such as proteins, saponins, isoflavones, phytic acids, trypsin inhibitors and phytosterols. Among them, saponins in soybeans have attracted considerable interest because of their health benefits. Soyasaponin A and B are the most abundant types of saponins found in soybeans along with soyasapogenol (aglycone), which is a precursor of soyasaponin. The main purpose of this experiment was to determine the concentration of soyasapogenol in soybean seeds and sprouts as a function of seed size, usage, seed coat color and seed cotyledon color. The 79 Korean soybean varieties were cultivated at Yesan of Chungnam in 2006 for the analysis of soyasapogenol using HPLC with Evaporative Light Scattering Detection (ELSD). The total average concentration of soyasapogenol was 1313.52~mug~;g-1 in soybean seeds and 1377.22~mug~;g-1 in soybean sprouts. Soybean sprouts were about 5% higher than soybean seeds in average total soyasapogenol concentration. In the process of sprouting, the average soyasapogenol A content decreased by approximately 1.6%, but soyasapogenol B and total soyasapogenol increased by 8.31% and 4.88%, based on the content of soybean seeds. When classified according to the size of seeds, the total soyasapogenol concentration of soybean seeds were not significantly different (p<0.05) On average, small soybean seeds were increased by as much as 103.14~mug~;g-1 in sprouting process. As a function of the use of the seeds, The total soyasapogenol in soybean seeds were significantly different (p<0.05). While, the soybean sprouts were not significant different (p<0.05). Altogether, sprout soybean seeds show the greatest change in content during the germination process. When seeds with different coat colors were compared, the total soyasapogenol concentration of soybean with yellow seed coats (1357.30~mu g~;g1 ) was slightly higher than that of soybean with black (1260.30~mug~;g-1 ) or brown (1263.62~mug~;g-1 ) seed coats. For the color of the cotyledon, the total soyasapogenol concentration was significantly increased in green cotyledon during the germination and seedling process. The results of this study suggest the functional characteristics of soybeans through quantitative analysis of soyasapogenol. In addition, the concentration of soyasapogenol exhibited a change during the germination process, which was evaluated by the nutritional value of the soybean sprouts.
Phenolic compounds are naturally occurring secondary metabolites from various plants. These compounds play important roles in plant such as antioxident, antibacterial and anticancer effects. The purpose of this research was to analyze the phenolic compounds contents in Kenaf leaf and stem using High Performance Liquid Chromatograpy. Kenaf is widely distributed throughout the country and third largest fiber crop. Recently, interest in this crop is growing. But, there is little research on their functional material. The obtained results showed that phenolic compounds were significantly various among the examined cultivars of Kenaf. Especially, phenolic compounds contents of leaf were higher than those of stem. The highest concentration of phenolic compounds was in the E41 leaf(24358.99㎍/g) and the lowest concentration was in the Kenaf (in korea) stem(988.21㎍/g). The results of this experiment showed that its contents were different in leaf and stem. These results suggested that Kenaf can be used for functional plant.
Gangjeong is a Korea traditional sweet cookie. Since long ago, Koreans have made Gangjeong at home whenever they have a feast such as a wedding or an ancestral rite. Gangjeong is made of rice or brown rice, honey, and oil, and has many sizies and shapes. They soak the fried pieces of Gangjeong in malt syrup, and produce different colored cookies by covering them with flour made from sesame, beans, pine nuts, pine pollen or grains of boiled rice dyed various colors. Recently, types of Gangieong has varied as the well-being trends. This experiment was conducted to examine the concentration of phenolic compounds the 24 types of Gangjeong finished goods and raw material. The total average concentration of phenolic compounds was 2340.66㎍/g and the most and the least were detected in cinnamon powder(21143.10㎍/g) and rice(49.40㎍/g), respectively. Cinnamon, green-tea powder and others had the highest content of phenolic compounds in the raw materisls. And the finished gangjeong, rice plus curry products exhibited the highest content of total phenolic compounds(1221.60㎍/g). Among the individual phenolic compounds, benzoic acid showed the highest (379.24㎍/g) concentration and sallicylic acid was the lowest (0.34㎍/g) consitutent.
Cereal grains are sort of crops like barley, sorghum, millet, oats, rye and many others. Research of other scientists were shown that whole grain consumption helps lower the risk of cardiovascular disease, ischemic stroke and gastrointestinal cancers. They are rich source of carbohydrates, protein, fats and oils, and have many health-promoting components such as vitamins, minerals, and phytochemicals, which include phenolic compounds. This research was conducted quantitative analysis about phenolic compounds in 15 lines of cereals by high performance liquid chromatography(HPLC). Samples are 5 of Sorghum bicolor Moench, 5 of Setaria italica, 5 of Panicum miliaceum L. they are harvested from Kyungbook-bongwha in 2009. The phenolic compounds contents of Moktak-sorghum (1187.298㎍/g), Hwanggeumchal-sorghum(897.7417㎍/g) and Hwanggeum-sorghum(762.8306㎍/g) are the highst, those have dark color between Sorghum bicolor Moench. Analysis Among the contents of Sorghum bicolor Moench (+)Catechin(avg. 139.95㎍/g) and Benzoic acid(avg.116.695㎍/g) were higher contents than others. Myricetin(avg.46.15㎍/g) and Quercetin(avg. 47.4368㎍/g) were many contents in Steria italica. Chlorogenic acid(avg. 46.258㎍/g) and Quercetin(avg. 43.0496㎍/g) were many contents in Panicum miliaceum L. The result of this experiment is considered to be the basic data of the functional food manufacturing by utilizing domestic cereal grains.
In order to assess antioxidant capacity in relation to seed traits of rice (Oryza sativa L.), ninety-six varieties were examined for antioxidative activity of brown rice grain using superoxide dismutase (SOD), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and thiobarbituric acid (TBA) assays. Overall, average total activities measured by the three methods were of very wide range between 64% and 13%. Significant differences were noted depending on the variety and evaluation method. Rice varieties with foreign origin, middle maturity, colored hulls, and colorless awn exhibited statistically significant higher total activity. As for the measurements, total activity was significantly correlated with SOD (r=0.29***), DPPH (r=0.80***) and TBA (r=0.76***). Between the three activities, SOD was not positively correlated with DPPH (r=0.15*), while TBA was significantly correlated with DPPH value (r=0.51***). DPPH (55.20%) and TBA (50.36%) were significantly higher in foreign rice, while SOD activity (44.29%) was significantly higher in domestic rice. However, an average total activity was significantly higher in foreign rice (47.31%) than in domestic rice (35.92%). SOD, DPPH and TBA activities of middle maturity in maturity time were the highest total activity (44.96%) and significantly differed from the other two groups. Total activity was significantly higher in rice with a colorless awn (42.18%) than with a colored awn (35.87%).