There are various types of level gauging method such as using float, differential pressure, hypersonic, displacement and so on. In this study, among them, the method utilizing the differential pressure was reviewed. The strengths include: the differential pressure type level gauge can measure the level without direct contact of the sensor with media. That is to say, the level can be measured even if the sensor is far away from the tank. And regardless of the size of the tank, the level can be measured if the pneumatic pipes are installed. The weaknesses include: the sensor needs intermedium to recognize the level. The intermedium utilizes a fluid, which is compressed air. It is difficult to handle that compressed air has the properties of a gas. And to make compressed air needs compressor, tank and pneumatic pipes. So if you have many tanks, you need to install exponentially the pneumatic pipes. As well, level measurement range is limited to the points where the pneumatic pipes of the tank is installed. And if a compressed air that supplies to the sensor leaks, uncertainty will increase. A compressed air is colorless and odorless, so it’s difficult to pinpoint the leak. Finally, events like cracks and clogging can cause inaccurate measurement. Rather than using only differential pressure, it is better to use another measurement method according to the situation of the facility.

In this study, plant regeneration through in vitro culture from plantlet stems of Yooja (C. junos Sieb.) and trifoliate orange (P. trifoliata Rafin.) was attempted to make mass-production system of virus-free plants having the same genotype with mother plant. In order to investigate physiological change depending on the developmental stage of plant regeneration, the changes of total protein, peroxidase and esterase activity and their isozyme patterns as well were examined in 1/2 MS medium. The results are as follows : 1. The MS medium for the optimal callus induction and shoot formation was utilized. The medium was supplemented either with 2,4-D and Kinetin or with BA and NAA. The optimal concentrations were the combination of 1.0mg/ 2,4-D +0.3mg/ Kinetin and 1.0mg BA +0.3mg NAA in callus induction and shoot formation, respectively. 2. For the plant regeneration from somatic embryos, 1/2 MS medium was used with supplements of growth regulators (free, 1.0mg/ IBA +1.0mg/ BA ,0.5mg/ IBA +0.5mg/ BA). Shooting and rooting were the best in the treatment of 0.5mg/ IBA and 0.5mg/ BA combination. 3. The total protein content has a tendency of increase with the developmental stage of embryo, but it was decreased at the plantlet. Also it was the highest at 8 and 6 weeks stage in C. junos Sieb. and P. trioliata Rafin, respectively. In the SDS-PAGE pattern of protein, C. junos Sieb. showed bands of 29.0 and 40kDa at 10 weeks. The 45,66 and 97.4 kDa bands at 10 weeks of culture were shown in P. trifoliata Rafin. 4. The highest esterase activity was shown at the 6 and 8 weeks of culture in C.junos Sieb. and P. trifoliata Rafin.., respectively. 5. Esterase isozyme patterns were shown difference according to the developmental stage. In C. junos Sieb. a new band was observed at pl 7.7 following 4 weeks culture. On the other hand, new bands in P. trifoliata Rafin. were observed at pl 7.5~6.5 following 4 and 6 weeks culture, respectively.