To evaluate the influence of Meloidogyne incognita on hot pepper, hot pepper seedlings (Capsicum annuum cv. Supertankang) were planted in wooden box microplot (30×40×15cm, L×W×H) and growth and fruit yield were measured in a greenhouse condition from June to Nov., 2010. The initial population densities(Pi) of root knot nematode in the microplots were adjusted to 0, 10, 30, 100 and 300 second-stage juveniles (J2) in 100cm3 of soils. The fruit yields were inversely correlated with Pi and the relationship of total fruit yield to Pi could be adequately described by a linear regression equation, Y=0.18-0.039×LOG10(Pi+1), R2=0.49**. The Pi of 30 J2/100cm3 root knot nematode before planting caused yield loss exceed the economic threshold of non-fumigant nematicide application compared. Non-fumigant nematicides for M. incognita should be applied level around 30 J2/100cm3 soil for proper cultivation.