Antler of deer have been used in Asia as a dietary supplementary or alternative medical substance. The antler contains various biological active substances, including ganglioside has been known to promote growth, bone hyperactivity, immune function, protein synthesis and various pharmacological action. Therefore, this study was to investigate the effect of antler on productivity of broiler chicks. Feeding trial has a number of 240 broiler chicks was conducted to investigate the effect of antler extract (AE) treated feeds on body performance, breast meat composition and characteristics in broilers. Each treatment had 15 chicks with 4 replications. The supplementation level of AE treated feeds was 0.3, 0.6 and 0.9% in the experimental diets. The results obtained were summarized as follows: Average body weight gain was significantly (p<0.05) increased in broilers fed with AE compared to that of control during overall periods. Especially, the body weight gain of 0.9% AE treated chickens showed highly increased by 6.0% compare to the control. Average feed intake was not significantly different among the treatments. Average feed efficiency was significantly (p<0.05) improved by 4% in broiler chicks fed with AE compare to that of control. Average breast meat composition and concentration of cholesterol were also not different by feeding the AE. In breast meat characteristics, the shear force (kg/0.5inch2) of breast meat showed similar results in all treatments. The cooking loss of breast meat was significantly lower in the chick fed AE than that of control. But the water holding capacity (%) of breast meat was significantly (p<0.05) higher in the chicks fed AE than that of control. In breast meat color of chicks were not significantly different in all treatments. The thiobarbituric acid reactive substances (TBARS) and volatile base nitrogen (VBN) value were not showed different with all treatments. Also, the fatty acids composition of breast meat were showed similar results in all treatments. In conclusion, the dietary feed of AE tended to improve the body weight gain and feed efficiency, but did not influenced breast meat characteristics of broiler chicks

This study was to examine expression of the recombinant full-length adiponectin (recombinant adiponectin) in insect ovarian cell culture system and to characterize structural properties of the recombinant adiponectin secreted in medium. Gene construct encoding the recombinant adiponectin contained N-terminal collagen-like domain (110 Amino Acids, AAs), C-terminal globular domain (137 AAs) and C-terminal peptides for detection with V5 antibody (26 AAs included adaptor peptide) and purification using the 6xHis tag (6 AAs). The approximate molecular weight of the product (monomer) was 35 kDa. Molecular mass species of the expressed recombinant adiponectin were monomer (～35 kDa), dimer (～70 kDa), trimer (～105 kDa) and hexamer (～210 kDa). The major secreted species were the LMW forms, such as monomer, dimer, and trimer. There was MMW of hexamer as minor form. HMW multimers (～300 kDa) were shown as a tracer or not detected on the SDS-PAGE in several experiments (data not shown). The multimer forms in this study were not compatible to those in animal or human serum and adipose tissue by other researcher’s study in which the major multimer forms were HMW. By protein denaturing experiments with reducing reagent (β- MeOH), anionic detergent (SDS) and heat (95℃) on the SDS-PAGE, not all adiponectin multimers seemed to have disulfide bond linked structure to form multimers. The recombinant adiponectin which expressed in insect ovarian cell culture system seemed to have the limitation as full physiological regulator for the application to animal and human study.