Egg SCP ( –47.2 ±1.0℃) of Red-spotted Apollo butterfly, Parnassius bremeri, was lower than larva(–35.0 ±0.9℃). The difference (12℃) between egg and larva was revealed by chorion through scanning electron microscope (COXEM EM-30, Korea). But considering of about 6 months’ pharate 1st instar in egg from June to November, function of egg seems not to be overwintering mechanism but rather estivation. In order to identify estivation function of chorion heat stress was carried out. Experiment was conducted on the 4 conditions such as high temperatures of 25, 35, 45 and 55 °C for 2 hour, then allowed them to recover at 25 °C for 1 hour. Total 65 samples 0f 31 eggs and 34 pharate 1st instar out of an egg were used and replicated 3 times. All pharate 1st instar in egg and pharate 1st instar out of egg survived at 25℃ and 35℃. In case of 45℃ survival rate of pharate 1st instar in an egg (91.6±3.5%) was significantly higher than those (37.8±9.4%) of pharate 1st instar out of an egg. All was dead at 55℃.

The early spring swallowtail, Luehdorfia puziloi (Lepidoptera: Papilionidae) is univoltine with adult emergence in early spring and the larvae feed on the genus Asarum (Aristolochiaceae). This study was conducted to investigate temperature effect on adult emergence of overwintering L. puziloi pupae at five different constant temperatures (15, 20, 25, 27.5 and 30℃) with photoperiod 10:14(L:D) and relative humidity 60% by using 150 pupae, 2013 in Holoce Ecosystem Conservation Research Institution (HECRI). The lower developmental thresholds of female, male, and both sexes combined were 6.131, 8.64, and 8.088℃, respectively. Developmental period of overwintering pupae to adults decreased with increasing temperature from 15 to 30℃. Mortality of five different constant temperatures (15, 20, 25, 27.5, and 30℃) was 13.3, 3.3, 16.67, 13.3 and 13.3%. Especially mortality of constant temperature 25℃ was higher than other conditions and survival rate 20℃ was the highest. Thermal constants of female, male and both sexes combined were 207.11, 125.97 and 150.18DD, respectively. The relationship between the developmental rate and temperature was fitted by linear and five nonlinear developmental rate models (Logan 6, Lactin 1, 2 and Briere 1, 2). The development variation of overwintering pupae was described by the two-parameter Weibull distribution model. The adult emergence of overwintering L. puziloi pupae in spring can be explained and predicted by using developmental rate models and developmental distribution model.