Species of root knot nematodes, Meloidogyne spp. were identified through the PCR amplification and sequence analysis of mitochondrial DNA (mtDNA) region between COII and lrRNA genes. Root knot nematodes were extracted from 5 plant samples [kiwi (Japan), rhododendron (Japan), cornus (Japan), ficus (China) and jasmine (Vietnam)] that were collected from Plant Quarantine Station in Korea. At first, species was identified using morphological characters, such as the length and shape of stylet, the tail length and the perineal patterns. Secondary, single individuals of either female and juvenile collected from plant tissues were used for PCR and further sequencing analysis. The results showed that cornus, jasmine and ficus plants were infected by M. incognita, and kiwi and rhododendron plants were infected by M. interolobii.