본 연구를 통해 국내에 분포하는 중기문응애류 중 파리응애과의 Holostaspella crenulata Krantz, 1967 (톱니무늬파리응애, 신칭) 와 화살응애 과의 Lasioseius floridensis Berlese, 1916 (가슴선화살응애, 신칭) 를 처음으로 확인하고, 각 종에 대한 분류학적 진단과 분포정보, 성충의 현미경사 진과 도판을 제시하였다.

Beetles are one of the most effective transportation for phoretic chelicerata. They use various methods to attach themselves to their hosts, such as developing ventral sucker plates, grasping with their claws and chelicerae, or hiding beneath the elytra. Recently, we discovered new species of two genera: Antennoseius (Mesostigmata: Blattisociidae) and Gaeolaelaps (Mesostigmata: Laelapidae) which are hiding under the elytra of Carabidae hosts. In this study, digital images of the beetles, and mites, along with brief diagnostic information and line-drawing plates will be provided.

Ixodidae에 속하는 일부 참진드기들은 중증열성혈소판감소증후군(severe fever with thrombocytopenia syndrome; SFTS)을 비롯한 질병을 매개하는 공중보건학적으로 중요한 해충이다. 우리나라에서는 2013년 이후로 SFTS 환자 발생수가 지속적으로 증가하는 경향을 보이며, 경상북도는 전국적으로 2번째로 많은 SFTS 환자가 발생하는 지역이다. 본 연구에서는 2019년 경북 상주 지역의 진드기 분포를 조사하기 위해 시민의 생활 반경 주변을 도심 녹지지역, 관리 취약지역 및 농촌지역으로 구별하여 flagging 방법을 통해 진드기를 채집하였고, 채집 진드기내 SFTS 바이러스 보균 여부를 조사하였다. 채집된 진드기 수를 Collection Index (CI = tick number / 1h / 2 people)로 산출한 결과, 상주시 내 총 26개 지점에서 작은소피참진드기와 개피참진드기, 일본참진드기를 포함하는 총 CI 143의 진드기가 채집되었고, 그 중 작은소피참진드기가 96.5%(CI 138)로 우점종으로 확인되었다. 채집된 진드기의 약 92%(131 CI)는 인적이 드물고 시청이나 관계 당국의 관리가 이루어지지 않는 관리 취약지역에서 채집되었으나, 도심의 녹지지역와 농촌 지역에서는 8.4%(CI 12)의 진드기만 관찰되었다. 총 CI 143의 진드기를 26개 pooling 한 후 SFTS 바이러스 존재 여부를 조사하였으나, 모두 음성으로 확인되었다. 본 연구의 결과는 지역주민들로 하여금 진드기 매개 질병으로부터 안전한 생활을 하기 위한 권고 자료로 활용할 수 있을 것으로 기대된다.

Haemaphysalis longicornis (H. longicornis) is the most dominant species that accounts for more than 90% of domestic ticks in Korea. It is also a main mediator and propagator of Severe Fever with Thrombocytopenia Syndrome virus. In order to efficient control of H. longicornis, it is essential to obtain the genetic information of pesticide target genes and determine the mutations putatively involved in insecticide resistance. Acetylcholinesterase (Ace) is a target gene of organophosphorus and carbamate insecticides, but no sequence information of Ace is available to date in H. longicornis. Therefore, in this study, we analyzed the transcriptome of H. longicornis and obtained the sequence information of three Ace genes (HlAce1, HlAce2 and HlAce3). Based on the comparison analysis with sequences of multiple ace genes from other hard ticks, mites and insects, three HlAces were grouped in Acari Ace1, Ace2 and Ace3 clade, respectively. Among three HlAces, HlAce1 seems to be major enzyme because of its conserved amino acids involved in catalytic function.

We monitored the population of Haemaphysalis longicornis, in Andong, Gyungbuk from April to November, 2018. Among total 2,994 ticks collected for 8 months, H. longicornis 1,677(56%), H. spp Larva 1,074(35.9%), H. flava 213(7.1%), Ixodes nipponensis 30(1.0%) were identified. In addition, considering the environment, 1,727(57.7%), 907(30.3%), 192(6.4%) and 168(5.6%) ticks were collected in the grassland, graves, copse, mountain path, respectively. In the pathogen diagnosis with PCR using SFTS virus specific primers, positive viruses were detected in H. longicornis, H. flava and H. spp from June to October. The minimum field infection rate of June, July, August, September and October were 0.4%, 0.8%, 1.2%, 0.8% and 2.3%. respectively.

Heamaphysalis longicornis is a major vector for Severe Fever with Thrombocytopenia Syndrome (SFTS) virus and the density of the vector has been increasing because of the climate change. The incidence of fatalities due to SFTS is increasing every year. In this study, to evaluate the SFTS transmission by ticks, the density of ticks mediating SFTS was monitored. Tick was collected every month from four different sites (Grass land, Mountain path, Grave, Copse) in Andong with the traps containing dry ice as CO2 attractants. Among 2,572 ticks of 3 species; H. longicornis, H. flava, and Ixodes nipponensis were most abundantly collected from April to August. H. longicornis is the richest species (92.8%), whereas Ixodes nipponensis was the least species (0.8%). The 54.5% of the sample were nymph stage and female/male ratio was 64.3%. According to the pathogene analysis, SFTS virus was detected from H. longicornis adult and larvae stages collected in July and August. In July samples, SFTS virus was detected only from grassland site, but the virus was observed in the sample from all four sites in August. For effective prevention of SFTS, the tick density should be continuously monitored based on the onset time of SFTS with the consideration of habitate, habit & life history of ticks.