This study was carried out to elucidate suitable substrate making of Lentinus lepideus for an artificial cultivation. We could obtain the highest yield of fruit body and shortest period of cultivation in the case of pine sawdust and deffated corn flour mixing substrate. And suitable mixing ratio of main substrate and additives was selected 90:10(v/v). Consequently, it is necessary to elucidate suitable environmental conditions in fruit body step for the good quality and stable production of this mushroom.