This study was carried out to screen high α-glucosidase inhibitor (AGI) activity pepper lines from EMS treated mutants of pepper. The optimal concentration of EMS for inducing mutants to pepper was 0.15M and the optimal treatment time of EMS was 2 hours. With this condition, many mutants which have high AGI activity were screened and especially leaf of two lines were showed 27 times inhibitory activity on α-glucosidase comparing with control. There is no difference between mutants and nornmal plant in growth. These results indicated that AGI activity of pepper leaf would be increased by mutation.