This study was carried out to investigate comparision anaylsis of metabolites in mandarin leave inoculated by Elsinoe fawcetii. For the analysis of metabolites, we performed using by UPLC-MS spectrometry in gradient eluent condition by acetonitrile and water. Flow rate was 0.4ml/min. Instruments conditions were followed by detection ion mode(positive ion), scan range(MS:m/z100-1000), spray voltage (4kV) ,capillary voltage(35v), capillary temp(300℃). Several flavones were detected in inoculated pathogen compared to control.

Three edible mushrooms; "Gumbit"(Pleurotus cornucopiae var. citrinopileatus) and Noeul"(Pleurotus salmoneostramineus) and Tricholoma spectabilis which are widely distributed in China, Japan and Korea. For the study of anti-aging activities, we were performed on adriamycin-induced cellular senescence model. Human dermal fibroblasts(HDFs) and human umbilical endothelial cells(HUVECs) treated with adriamycin for inducing aging were used for cytotoxicity and senescence-associated beta-galactosidase(SA-β-gal) activity assay.

Cordyceps militar is (Clavicipitales) is an edible mushroom which is widely distributed in China, Japan and Korea. Various phytochemical constituents, cordycepin, homocitrully laminoadenosine and sterols have been reported from this source and a wide range of biological activities, including antimicrobial, macrophage activation, anticancer, immune modulatory effects were studied. In a continuing search for bioactive constituents from Korean mushrooms, we performed a phytochemical investigation of the MeOH extract from the fruiting bodies of C. militaris. By repeated column chromatographic separation of the extract, fourteen compounds, were isolated. The identification and structural elucidation of the compound was based on NMR spectral data. anti-aging activities were performed on adriamycin-induced cellular senescence model. Human dermal fibroblasts(HDFs) and human umbilical endothelial cells(HUVECs) treated with adriamycin for inducing aging were used for cytotoxicity and senescence-associated beta-galactosidase(SA-β-gal) activity assay.