Spindlin1(Spin1), a meiotic spindle-binding protein that is highly expressed in cancer cells. Spindle-binding was dependent on its phosphorylation status, which was partially regulated by Mos/MAP kinase pathway. Nevertheless, the biologic roles of Spin1 in oocytes maturation are still largely unknown. For exploring the function of Spin1 in porcine oocyte maturation, Knockdown and overexpression methods were employed to the present study. Spin1 mRNA were enriched in maternal stages, from germinal vesicle - to 2 cell - stage, but sharply decreased after 4 cell stage, zygotic genome activation. Protein of SPIN1 was localized in spindle-chromatin complex during the metaphase I and metaphase II stages. Knockdown of Spin1 did not affect the first polar body extrusion, however, Spin1 depletion caused mitotic spindle defects, chromosome instability and pronuclear formation in metaphase II stage. Percentage of 2cell, 4cell embryos and blastocyst formation were significantly reduced in knockdown group compared with control, but cell numbers in blastocyst were no difference between control and knockdown groups. Another hand, Oocyte failed to maturation and induced metaphase I arrest following Spin1 over-expression. In conclusion, Spin1 is involved in the spindle formation and maintenance during oocytes meiotic maturation in pigs.

본 논문에서는 공공연구개발사업의 기술파급측정을 위한 이론적/방법론적 기반을 제공하기 위하여 기술파급현상을 고찰하고 이를 측정하는 방법론에 관하여 논의하고자 한다. 본 논문은 다음과 같이 구성된다. 첫째, 일반적인 기술파급메커니즘에 관하여 논의하며 파급현상 및 파급경로를 정리한다. 둘째, 기존에 문헌들에서 사용된 주요 방법론을 소개하고 방법론의 장단점을 논의한다. 셋째, 방법론과 기술파급현상에 대한 이해를 기반으로 기술파급현상과 측정의 문제를 고찰한다. 마지막으로 공공연구개발 사업의 기술파급효과 측정을 위한 선행조건을 제시한다. 본 논문의 목적은 연구자 및 정책입안자들에게 기술파급현상과 측정에 관한 이해를 넓히고 여러 방법론으로 측정된 기술파급측정의 결과를 올바르게 평가를 할 수 있도록 하기 위해서이다. 본 연구는 향후 공공연구개발 사업의 파급효과를 측정하고자 할 때 방법론적 한계를 인식하여 파급현상과 측정 결과의 간극(gap)을 인지하여 이를 기반으로 더 향상된 측정 방법을 고안하는데 도움을 줄 것이다.

This study was conducted to investigate runoff characteristics of non-point pollutants source at the urban and rural zones in sangju area. The monitoring was conducted with seven events for ten months and Event mean Concentration(EMC) and First Flush Effect(FFE) of SS and BOD were calculated on the result of the water quality parameters. During rainfall event, the peak concentrations of SS and BOD were observed after 3∼4 hours of rainfall in rural areas. Whereas, the peak concentrations occurred within 1∼2 hours after rainfall and then the highest concentration of NPS pollutants sharply decreased, showing strong first flush effect in urban areas. The cumulative load curves for NPS pollutants showed above the 45° straight line, indicating that fist flush effect occurred in urban areas. The mean SS EMC values of rural areas ranged from 0.9∼3.3mg/L, it was higher value when compare to urban areas. While the mean BOD values of urban areas were shown the highest values.