Twenty two common millet (Panicum miliaceum L.) varieties collected from Korea, China and Russia were investigated for their phylogenetic relationship using 5S ribosomal DNA sequences with a hope to provide the basic information on their exact origin. Sequences of 5S rDNA were isolated by PCR. The primers, 5s-rRNA1 and 5s-rRNA2, were designed to isolate the complete NTS. Genomic DNA amplification produced two fragments with different length, 900 bp and 400 bp fragments, confirming the presence of two types of 5S rDNA repeats that differed from each other in the length of the NTS region. Amplified DNAs of 400 bp fragment were subcloned and used for further investigation. The obtained NTS sequences ranged from 200 to 300 bp and homology of sequences among plant materials was much higher than long repeat. CLUSTALW multiple aligment of 5S rDNA sequences from 22 different common millets revealed the clear difference by their origin. And critically different areas with insert or deletion were also confirmed. Those sequence difference seems to be used for discrimination of cultivars from different origin and use as molecular markers for origin identification. In phylogenic tree construction, the clear classification was shown where the genotypes from China and Russia is positioned together and stay away from domestic genotypes.
This study was carried out to understand the genetic pattern of self-sterility, using a self-fertile variety Cheongyangnative 1 and nine self-sterile cultivars in cultivated boxthorn (Lycium chinense Mill., 2n=24). The inheritance of self-fertility (SF)wa
CODEX의 유기농업기준에 대하여 기본적으로는 공감하고 있으나, 아시아적 기후의 특성과 농가당 소유면적이 유럽이나 미주․오세아니아 지역에 비하여 상대적으로 너무 협소한 점을 감안하여 아시아의 유기벼 재배농가들에게 과중한 부담을 주지 않을 수 있는 선에서의 유연성을 부여해야 할 것이다.
윤작이나 휴경의 경우, 지역에 따라 벼딸기 등의 과채류벼토마토 등 과채류벼 행태나, 벼보리 또는 호밀 등의 사료 또는 녹비작물벼자운영 등의 사료