콩은 식물성 단백질 및 지방의 주요 공급원이고 콩 종실에는 기능성 성분이 많이 함유되어져 있어 소비 가 점차 증가하고 있지만 Lipoxygenase, Kunitz Trypsin inhibitor, 7S α′-subunit 단백질과 같은 성 분들이 존재하는데 이는 품질과 영양가치를 떨어뜨리고 섭취시 알러지를 일으키기도 한다. 콩에서 유전적 으로 이러한 성분이 결핍되어져 있는 유전자형의 선발은 품질이 우수한 콩 육종의 기초단계이다. “개척2 호”와 PI506876의 교배로부터 434개의 F2 종자를 얻어 F2 종자의 일부를 사용하여 SDS-PAGE로 각각 의 종자를 분석한 결과 Lipoxygenase와 Kunitz Trypsin inhibitor 및 7S의 α′-subunit 단백질이 모두 결핍되어져 있는 lx1lx1lx2lx2lx3lx3titicgy1cgy1 유전자형을 가진 종자를 선발하여 F2 식물체로 길러 성 숙 후 F3 종자를 수확하였다. F3 종자로부터 Lipoxygenase, Kunitz Trypsin inhibitor, 7S α′-subunit 단백질이 모두 결핍되어져 있음을 재확인하였으며 선발된 종자는 고품질 콩 품종 육성에 유용하게 활용될 것으로 기대된다.

Soybean [Glycine max (L.) Merr.] protein is excellent nutritional factors and is widely used for human and animal feed in the world. Glycinin(11S globulin) and ß-conglycinin(7S globulin) account for storage protein from 70% to 80% in soybean seed. ß-conglycinin is composed of α’, α and ß subunits, encoded by the genes Cgy1, Cgy2, and Cgy3, respectively. β-conglycinin protein exhibits poor nutritional and food processing properties. Lipoxygenase is responsible for the beany flavor and is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. The objective of this research was to select F2 seeds with lipoxygenase-free and low content of ß-conglycinin for breeding of high quality soybean cultivar. A total of 582 F2 seeds were obtained from the cross of 10F1(lipoxygenase-free, normal content of 7S protein) and T311(lipoxygenase-present, low content of 7S protein). Lipoxygenase and 7S proteins in mature seeds were detected by SDS-PAGE. The segregation ratio of 3 : 1 for Shr locus (418 Shr_ : 164 shrshr) were observed. This result shows that the Shr locus is controlled by a single gene. Among 164 shriveled seeds, 6 F2 seeds with lipoxygenase-free, α’ subunit-free, and low content of α and ß subunit were selected. Among 418 normal seeds, 6 F2 seeds with lipoxygenase-free, α’ subunit-free, and low content of α and ß subunit were selected. These results will be used in breeding program for improving high quality soybean cultivar.