콩은 식물성 단백질 및 지방의 주요 공급원이고 콩 종실에는 기능성 성분이 많이 함유되어져 있어 소비 가 점차 증가하고 있지만 Lipoxygenase, Kunitz Trypsin inhibitor, 7S α′-subunit 단백질과 같은 성 분들이 존재하는데 이는 품질과 영양가치를 떨어뜨리고 섭취시 알러지를 일으키기도 한다. 콩에서 유전적 으로 이러한 성분이 결핍되어져 있는 유전자형의 선발은 품질이 우수한 콩 육종의 기초단계이다. “개척2 호”와 PI506876의 교배로부터 434개의 F2 종자를 얻어 F2 종자의 일부를 사용하여 SDS-PAGE로 각각 의 종자를 분석한 결과 Lipoxygenase와 Kunitz Trypsin inhibitor 및 7S의 α′-subunit 단백질이 모두 결핍되어져 있는 lx1lx1lx2lx2lx3lx3titicgy1cgy1 유전자형을 가진 종자를 선발하여 F2 식물체로 길러 성 숙 후 F3 종자를 수확하였다. F3 종자로부터 Lipoxygenase, Kunitz Trypsin inhibitor, 7S α′-subunit 단백질이 모두 결핍되어져 있음을 재확인하였으며 선발된 종자는 고품질 콩 품종 육성에 유용하게 활용될 것으로 기대된다.

Background : The Schizonepeta which is characterized by short growth period is used for the pedicel as a medicinal plant. Its main ingredients are essential oil, monoterpenoid, and flavonoid. Major effects include fever, pain, anti-inflammation, and anti-allergy. There are no varieties developed so far, and it is necessary to develop standard varieties to supply domestically good varieties. In this study, the growth and yield characteristics of the genetic resources collected for cultivating good-quality varieties were tested. Three strains were classified into early, medium, and late life. Methods and Results : The research was carried out in the test packaging of the Medicinal Resources Research Institute (Hamyang) in Gyeongnam province. It was harvested in late May, early June, and mid June by flowering season. The harvest time was mid June, late June, mid July, late July, mid August, and late August. Flowering, growth characteristics and yield were investigated. Growth characteristics showed a tendency to increase with the delay of flowering and harvesting time, such as plant height, leaf length etc. As the flowering time and harvesting time were delayed, the number of trees increased. The selected early line showed 89.4 ㎝ in plant length, 38.3 g grain weight, medium line 69.5 ㎝, 61.0 g, late line 136.4 ㎝, 111.2 g. Conclusion : When the cultivars are selected for the selected strains, it is expected that the cultivars with high physiological activity characteristics will be able to develop the cultivars as high quality varieties and high yield varieties.

Background : When the Platycodon grandiflorum is applied before the rainy season, the increase of the incidence of the Platycodon grandiflorum root rot disease increases greatly. This experiment was carried out to reduce the incidence of rot root rot disease through the lodging protecting method. Methods and Results : Three-years-old Platycodon grandiflorum was subjected to four treatments with no treatment, net installation, cutting stem, and removing bud. No treatment was used as a control, and as a further control, netting was used in the Platycodon grandiflorum to set up a treatment with almost no lodging. In the case of cutting stem, the stem was cut off in the middle of June, leaving more than 60 ㎝ before flowering. In the case of removing bud, blooming just before, the bud was removed. As a result, the coverage rate was the highest at 36.9% in the non - treatment area in the middle of July after the rainy season and 0.4% in the net installation. Compared with the case of cutting a lot of stems, 12.7% of the stem was covered with stones, whereas the stalk was 31.8%, which was close to the untreated stomach. As a result of the change of morbidity rate per treatment, it showed a morbidity rate of 49.7% in case of net installation, compared with 60% or more morbidity rate in case of untreated. Conclusion : As a result, an anti-lodging technic has helped prevent the onset of root rot disease. Further research on how to prevent the lodging of Platycodon grandiflorum using cutting stem will be needed.

Background : Turmeric is a subtropical plant, which has a high temperature for growing, but if it continues to be high temperature, it will be turned into heat degradation phenomenon. In order to mitigate damage caused by high temperature, we investigate the damage caused by high temperature. Methods and Results : This study was carried out in the experimental packaging (April 2018 seeding) of the Gyeongnam provincial medicinal resource research institute and eight systems of Turmeric (Curcuma longa) As the main research items, temperature characteristics of treatment distinction and high temperature damage rate were investigated. From July to August 2018, the main weather condition was over 35℃ for more than 21 days and showed abnormal high temperature. CUR11 showed the highest mortality rate (9.1%), and CUR10 (44.8%) showed the highest mortality rate. The average number of CUR11 strain was 23.2%. The mortality rate was 4.9% at the 60 th day of the emergence period and 40.2% at the 75 th day after 2 weeks. The higher the loss rate, the higher the damage rate. When the coating was maintained, the coating surface showed a high temperature of 50℃ or higher. A test was conducted to confirm the heat-reducing effect by coating, as a result of the test, there was no significant difference in the removal rate compared to the control. Conclusion : In order to alleviate the effect of high temperatures on the growth of turmeric, it was found that the heat loss caused by the coating did not appear much in case of turmeric.

Background : Senna tora L. is an herbaceous annual foetid herb. There were many components s uch as emodine, chrysophanol, aloe-emodine, physcion, rhein, chrysophanic acid, obtusin, aurantio-obtusin, anthraquinone compounds. The seeds and leaves are used to treat skin disease and its see ds can be utilized as a laxative, as well as to good for the eyes. This experiment was focused on the storage characteristics for searching better condition. That's why it is very useful for making oriental medicine. It is needed for Good Agricultural Practices (GAP) certification. It is one of ce rtification condition. Many medicinal plants have almost no their storage condition for GAP. Methods and Results : There are two kinds of treatment for storage test. One is temperature. The other is time period. The temperature treatments are room temperature and 4℃. Time of storage periods are four parts. The first time period for storage is zero month. The Second is until three month. and the third is until six month. The fourth is until nine month. We used polyethylene for packing materials. We investigated moisture, ash, bacteria, Escherichia coli, Staphylococcus aureus, mold and chromaticity by them. The value of moisture and ash are under standard of permission as 7.8 and 5.1 respectively. There were almost no significance about test microorganism in the storage temperature condition. There were statistical significance on the bacteria and mold without Escherichia coli, Staphylococcus aureus according to storage period. In terms of stroage temperature and period interaction, there was statistical significance on the Escherichia coli. In addition, the mycotoxin (aflatoxin) was analyzed for nine month storage Senna tora L. The total aflatoxin value were 3.307 ppb and 3.334 ppb in storage temperature and period respectively Conclusion : About the characteristics of Senna tora L. in accordance with the storage treatments, there were differences along with time period of storage about the bacteria and mold. There was no aflatoxin until nine month storage.

Background : Schizonepeta tenuifolia Kitagawa is a herbaceous plant affiliated to labiate. It ha s many oil components which has antimicrobial activity for health with limonene, menthone, p ulegone, piperitenone, schizonepetoside A, schizonepetoside C, spatulenol. The planting area of which is comparatively small than other medicine crop as 5 ha area and 15MT in the nation. This experiment was focused on the storage characteristics for searching better condition. It is very useful for making oriental medicine. It is needed for Good Agricultural Practices (GAP) certification. It is one of certification condition for farmers. Many medicinal plants have almos t been no their storage condition for GAP. Methods and Results : This experiment was carried out to conform optimal condition for S. tenuifolia Kitagawa. There were two kinds of treatment for storage test. One is temperature. The other is time period. The temperature treatments are room temperature and 4℃. The storage periods are divided into four times. Their times continued three month until nine month. There used polyethylene for packing materials. This experiment was investigated to storage condition about moisture, ash, bacteria, Escherichia coli, Staphylococcus aureus, mold and chromaticity. The contents value of moisture and ash are under standard of permission as 9.7 and 6.2 respectively. There were almost no significance about test microorganism in the storage temperature condition. There was statistical significance on the bacteria according to storage period. In terms of stroage temperature and period interaction, there was statistical significance on the Staphylococcus aureus and mold. Conclusion : The effect of storage treatments on S. tenuifolia Kitagawa mainly focused to time period treatment and time period-temperature interaction treatments, there were differences along with time period of storage about the bacteria and interaction with storage temperature and period about Staphylococcus aureus and mold.

Background : Rehmannia glutinose Liboschitz is a perennial herb belonging to the family of Scrophulariaceae. Its roots have been used as a traditional medicine. For cultivation of varieties of Rehmannia glutinose Liboschitz, the pure line selection method, which is to select the best line among those cultivated in farms for pedigree breeding, replicated yield test and regional adaptation test before registering as a new variety, is widely used. Until now, there are 8 registered varieties of Rehmannia glutinose Liboschitz in Korea. The needs for development of disaster-resistant varieties with better chances of surviving when high temperature, salts and disease are increasing. Therefore, this study focused on cultivation of disaster-resistant varieties among those selected for their disaster tolerance and salt tolerance through regional adaptation test. Methods and Results : As a result of examining the incidence of pest insects of the selected 1 - 2 years old varieties used in the study, among the 2-years-old crops, RGES 20 showed the lowest rate in ground parts. Among the 1-year-old crops, the lowest rate incidence of pest insects in ground parts was observed in RGES 23. In addition, RGES 20 and RGES 23 showed superior growth in under ground part with the highest dry root amount. Conclusion : Based on the above results, RGES 20 among the 2-years-old crops, RGES 23 among the 1-year-old crops showed superior growth in under ground part with the highest dry root amount.

Background : Panax ginseng C.A. Meyer belong to Panax species Araliaceae and a perennial plants. It grows in Far East Asia such as the Maritime province in Russia, the Manchuria reg ion of China, and the Korean peninsula. Panax ginseng C.A. Meyer is cultivated by seed sow ing or transplanting-culture, but seedling standing rate is as low as 50%. Therefore, it is impo rtant to increase seedling standing rate. This study was performed to research of plug-seedling cultivation techniques of Panax ginseng C.A. Meyer for increase of seedling standing rate. Methods and Results : Testing material, Panax ginseng C.A. Meyer seed, was sown on 5 kin ds of seedling trays (72, 105, 128, 162, 200 cells) in the middle of February 2016. It was gr own in the seedling green house in medicinal plant resources research department of Gyeongs angnam-do Agricultural Research and Extension Services. Four months after sowing, the seedli ng growth characteristics such as seedling standing rate and seedling withering rate were inves tigated. After transplanting in the mountain area of Hamyang-gun, growth characteristics of the root length, root weight, and other factors were investigated at the beginning of November. A s a result, seedling standing rates were 50.1% to 56.2% and seedling withering rates were 25. 2% to 35.9%. Among the five ports, the seedling standing rate of 105 cells and 72 cells was 56.2% and 55.7%, which were higher than others. In the case of 105 cells, however, seedling withering rate was 35.1% higher than that of 72 cells. Seedling leaf length and width were lo nger at 72, 105, 128 cells than at 162, 200 cells and seedling root length, root fresh weight and shoot fresh weight were the highest at 72 cells. Roughly, the more tray cells, the less se edling growth. After harvesting, among the five ports, root fresh weight and root dry weight per 1 hill of 128 cells were 0.36 g and 0.11 g, respectively, which were higher than those of other cells. Conclusion : The growth characteristics of Panax ginseng C.A. Meyer considering the seedling standing rate and seedling withering rate, among the five ports, 72 cells was most suitable. A mong the five ports, considering root fresh weight and root dry weight after harvesting, 128 c ells was most suitable.

본 연구는 대전광역시와 울산광역시에 소재한 8개 공립유치원 13학급에 재원중인 5세 학급 유아 255명(남아: 120명, 여아: 135명)을 대상으로, 취학 전 아동의 수용언어 및 표현언어 발달 척도에서 유아의 생활연령에 따라 언어발달을 어떻게 보이고 있으며 발달경향은 지역과 성별에 따라 차이가 있는지를 알아보고자 하였다. 그 결과, 첫째, 5․6세 유아의 언어능력은 수용언어 능력이 표현언어 능력보다 먼저 발달하고 있었다. 둘째, 수용언어 능력은 5세 1개월부터 발달이 이루어지고, 표현언어 능력은 5세 7개월 이후부터 발달이 이루어지며, 6세 이후가 되면 더 높은 수준의 발달을 보이고 있는 것으로 나타났다. 셋째, 유아의 언어능력은 생활연령에 따라 지역과 성별에서 차이를 보이고 있었으며, 5세 7개월～5세 12개월, 6세 1개월～6세 6개월에서 차이가 있는 것으로 나타났다.

인체내에서 소화불량 및 알레르기 반응을 일으킴으로써 콩의 품질을 저하시키는 물질인 Kunitz trypsin inhibitor(KTI) 단백질이 결핍되고, P34 단백질을 적게 함유하는 콩 계통을 선발하기 위해서, 현재까지 보고되지 않은 KTI 단백질의 유무와 P34 단백질 함량간의 유전관계에 대한 정보를 얻기 위하여 본 연구에서 얻어진 결과는 다음과 같다. 1. 07B1과 PI567476의 교배를 통해 얻어진 479개의 F2 종자를 대상으로 SDS-PAGE를 이용한 KTI 단백질의 유무를 확인한 결과, KTI 단백질이 존재하는 종자의 수는 353개, 결핍된 종자는 126개로 3 : 1로 분리하였다. 2 Western blot을 이용한 P34 단백질의 함량을 확인 한 결과, P34 단백질 함량이 보통 또는 높은 종자가 363개, 함량이 낮은 종자는 116개로 P34 단백질 함량에 대한 유전분리비는 3 : 1로 나타났다. 3. 전체 F2 종자 479개 중에서 KTI 단백질이 존재하며, P34 단백질 함량이 보통 또는 높은 종자가 266개, KTI 단백질이 존재하고 P34 단백질 함량이 낮은 종자가 88개, KTI 단백질이 결핍이고 P34 단백질함량이 보통 또는 높은 종자가 102개, KTI 단백질이 결핍이며 P34 단백질 함량이 적은 종자가 23개로 9:3:3:1의 분리비에 적합하여 KTI 단백질 유무와 P34 단백질 함량간에는 독립유전을 하였다.

본 연구는 자기존중감 기르기 교육활동 경험이 유아의 유치원 적응에 어떠한 영향을 미치는지를 밝히고자하는데 목적이 있다. 만 5세 유아 38명을 실험집단(19명)과 통제집단(19명)으로 하여 13주 동안 유아 1인당 주 2~3회, 총 31회의 실험처치가 이루어졌다. 실험집단 유아들에게는 자기존중감 기르기 교육활동을 실시하였고, 통제집단은 생활주제에 따른 유치원 일과운영에 맞추어 활동하였다. 그 결과, 자기존중감 기르기 교육활동 경험이 유아의 유치원 적응과 하위요소 중 친사회적 행동, 유치원 환경에서의 긍정적 정서, 유치원 생활에서의 적응에 긍정적인 영향을 미치는 것으로 나타났다.