경상남도농업기술원 화훼연구소에서 2021년 화색이 연황 색이며 화심이 녹색인 미니 절화용 거베라 품종 ‘크림쿠키’ (Cream Cookie)를 육성하였다. ‘크림쿠키’ 품종은 2014년 황색 미니 ‘Sun City’를 모본으로, 백색 미니 ‘Blandy’를 부 본으로 인공교배를 실시하여 육성된 품종이다. 2021년까지 생육 및 개화 특성검정과 기호도 조사를 실시하였으며 ‘크림 쿠키’의 생육 및 개화특성을 대조품종인 ‘Sun City’와 비교하 였다. ‘크림쿠키’는 연황색(RHS 4C) 꽃잎과 녹색 화심을 가진 반겹꽃 거베라 품종이다. 화폭이 7.1cm인 작은 꽃이며, 화경 장은 58.9cm였다. 화경 직경은 상부 0.4cm, 하부 0.7cm 였 다. 외부설상화의 길이는 2.8cm이며 폭은 0.8cm였다. 개화 소요일수는 65.7일로 ‘Sun City’에 비하여 18.8일 빨랐으며, 첫 개화시 엽수는 22.4매였다. 연간 주당 절화수량은 102.3 본으로 ‘Sun City’의 82.0본에 비하여 20.3본이 많았다. 절 화수명은 17.8일로 ‘Sun City’보다 4.1일 더 길었다. ‘크림쿠 키’는 화폭이 7.1cm의 미니 품종으로 연간 주당 100본 이상 절화 생산이 가능하다. 또한 수확 후 꽃 캡 씌우기, 꽃목 보강 등 추가적인 작업을 생략할 수 있어 생산비 절감이 가능하므 로 농가보급 확대가 기대된다.

수출용 스프레이 장미 신품종을 육성하기 위해 경남농업기술원 화훼연구소에서 2012년 분홍색 스프레이 장미 ‘Pink Sensation’을 모본으로, 오렌지색 스프레이 장미 ‘Orange Jam’ 을 부분으로 하여 잿빛곰팡이병 민감성이 약하고 수송성과 생산성이 좋은 황색 스프레이 장미 ‘Egg Tart’를 육성하였다(품종보호 제7791호). ‘Egg Tart’의 화형은 불규칙한 원형이고, 잎색은 짙은 녹색으로 광택이 강하였으며, 잎 수는 15매, 잎의 길이는 12.3cm로 대조품종 ‘Yellow Tea’에 비해 작았다. 잎의 형태적인 면에서 대조품종과 뚜렷한 차이를 보였는데 ‘Egg Tart’는 소엽 가장자리의 물결모양이 크지 않고 정단부 소엽이 좁은 타원형이었으며, 특히 정부의 모양은 매우 뾰족한 형태를 보였다. ‘Egg Tart’의 절화수량은 152본(㎡/년), 절화장은 78cm, 꽃잎수는 78.8매, 절화수명은 14.4일로 길어 스프레이 절화장미로서 대조품종 ‘Yellow Tea’보다 우수하였다. ‘Egg Tart’의 수출품종으로서 가능성을 확인하기 위해 같은 황색 품종인 ‘Yellow Babe’와 함께 장미 수출업체 재배온실에서 2017 년부터 3년간 실증재배를 하였고 생산된 절화는 내수시장과 일본 수출 시장에 출하하였다. 시험 결과 절화수량과 소화수는 두 품종간 차이가 없었으나 절화길이와 꽃 직경의 크기는 ‘Egg Tart’가 컸으며, 시장 가격도 내수시장과 일본 시장이 각각 송이당 845원, 758원으로 ‘Yellow Babe’ 보다 높았다. ‘Egg Tart’의 잿빛곰팡이병에 대한 민감성을 확인하기 위해 인위적으로 잿빛곰팡이병 균주를 접종하여 병 발생 및 확산 정도를 조사한 결과 대조품종인 ‘Yellow Babe’는 병원균 접종 후 1일 부터 감염이 되었으나 ‘Egg Tart’는 2일부터 감염되었고, 접종 후 4일에 ‘Yellow Babe’는 100% 감염되었지만, ‘Egg Tart’는 38.9%로 대조품종에 비해 상대적으로 감염이 적고 확산 속도도 느렸다. 이상의 결과를 종합해볼 때 황색 스프레이 장미 ‘Egg Tart’는 기존 수출품종 ‘Yellow Babe’를 대체 가능한 품종으로 판단된다.

본 연구는 생산자와 시장의 기호성이 우수한 스프레이 장미 품종 ‘Haesal’의 유용한 화색변이 품종을 단기간에 육성하기 위해 삽수와 발근삽목묘에 각각 감마선을 처리한 후 변이 발생 양상과 주요 화색변이의 생육특성을 조사하기 위해 실시하였다. 감마선 처리 시료 형태에 상관없이 선량이 높아질수록 식물체 치사율은 높아졌고 신초의 발생량과 신초 생장량이 감소하였다. 50% 치사선량(LD50, Lethal dose 50)은 삽수는 70Gy부터, 발근삽목묘는 90Gy부터였고 변이 발생률은 삽수와 발근삽목묘에서 각각 2.6%, 3.1% 였으며, 변이형태는 양쪽 모두 완전변이체, 키메라, 그리고 꽃 형태변이였다. 감마선을 처리한 삽수로부터 ‘Haesal’과 거의 유사한 특성을 가진 화색변이 MUL-1과 MUL-2를 각각 70Gy와 50Gy 선량에서 유기하였고 특성을 조사한 결과 꽃 직경과 꽃잎수, 절화수량은 ‘Haesal’에 비해 조금 적었지만 화형과 잎의 특성, 절화장 등은 ‘Haesal’과 차이가 없었다.

The purpose of this study is to investigate EU, UK and USA’s laws and standards related to safety criteria for commercial kitchen machines. The study was based on literature survey and web surfing. The results revealed that EU has relevant directives by kitchen machines and harmonized standards according to the directives. The directives and harmonized standards are translated into the laws and standards of EU member countries, respectively. The kitchen facility relevant legal systems of UK and USA do not prescribe the safety devices or measures, but only the basic health and safety requirements. The requirements were forcefully implemented through the certificate systems such as CE(Confommite European), UL(Underwriters Laboratories), etc. Only products with CE, UL or NRTL(ationally Recognized Testing Laboratory) certificate marking can be placed on the market of EU and USA, or put into service. For achieving the certificates, all requirements regulated in the relevant standards should be met. The standards of UK and USA were presented by kitchen machines or by standards themselves, respectively. Safety devices required by the standards were also summarized by kitchen machines and their risk factors.

우리나라 자연 상태의 노지재배에서 종자번식이 되 지 않는 크로코스미아의 기내증식을 위하여 2,4-D, NAA, kinetin, BA 처리가 캘러스 및 소자구 형성 등 에 미치는 영향을 조사하였다. 어린자방 배양에서 캘러 스 형성율은 BA와 2,4-D 혼합 처리에서 1~28%로 나타났으며 기내 뿌리 발생은 2,4-D, NAA 단독처리 에서 양호하였다. 소화를 이용한 배양에서는 kinetin +2,4-D, kinetin+NAA 조합에서 뿌리만 형성되기도 하 였다. rhizome 배양에서 줄기 형성에는 kinetin 단독 처리, kinetin+2,4-D 혼합처리가 양호하였으며 캘러스 형성에는 2,4-D 단독처리, 2,4-D+BA, 2,4-D+kinetin 혼합처리에서 25~92.8%의 형성율을 나타냈으며 corm 형성에서는 kinetin 처리와 kinetin + 2,4-D 0.1 mg·L-1 처리구에서 30~70%의 높은 형성율을 보였다.

나리속(屬) 식물의 엽초는 품종에 따라 엽위별 형태와 숫자가 큰 차이를 보여 품종별로 식재깊이에 따른 엽초수를 조사하고, 엽초를 제거한 후 생육 반응을 알아보았다. 품종별 엽초는 0cm로 식재한 경우 'Jolanda'는 6매엽, 'Dream Land'는 5매엽과 'Casablanca'는 11매엽으로, 6cm로 식재한 경우 'Jolanda'는 6매엽, 'Dream Land'는 5매엽과 'Casablanca'는 14매엽으로 밝혀져 'Jolanda'와 'Dream Land'는 식재깊이에 따라 엽초수의 차이가 없었으나 'Casablanca'는 6cm로 식재한 것이 0cm로 식재한 것에 비해 엽초가 3장이 더 많아졌다, 한편 엽초를 제거함으로써 'Le Reve'와 'Casablanca' 두 품종 모두 초장이 작아졌으며 stem root의 생육이 나빠졌다. basal root의 생육과 꽃수는 품종별로 다른 양상을 나타냈으며 줄기 직경, 개화소요일수와 꽃의 크기는 차이가 없었다.

Background : Panax ginseng C.A. Meyer belong to Panax species Araliaceae and a perennial plants. It grows in Far East Asia such as the Maritime province in Russia, the Manchuria reg ion of China, and the Korean peninsula. Panax ginseng C.A. Meyer is cultivated by seed sow ing or transplanting-culture, but seedling standing rate is as low as 50%. Therefore, it is impo rtant to increase seedling standing rate. This study was performed to research of plug-seedling cultivation techniques of Panax ginseng C.A. Meyer for increase of seedling standing rate. Methods and Results : Testing material, Panax ginseng C.A. Meyer seed, was sown on 5 kin ds of seedling trays (72, 105, 128, 162, 200 cells) in the middle of February 2016. It was gr own in the seedling green house in medicinal plant resources research department of Gyeongs angnam-do Agricultural Research and Extension Services. Four months after sowing, the seedli ng growth characteristics such as seedling standing rate and seedling withering rate were inves tigated. After transplanting in the mountain area of Hamyang-gun, growth characteristics of the root length, root weight, and other factors were investigated at the beginning of November. A s a result, seedling standing rates were 50.1% to 56.2% and seedling withering rates were 25. 2% to 35.9%. Among the five ports, the seedling standing rate of 105 cells and 72 cells was 56.2% and 55.7%, which were higher than others. In the case of 105 cells, however, seedling withering rate was 35.1% higher than that of 72 cells. Seedling leaf length and width were lo nger at 72, 105, 128 cells than at 162, 200 cells and seedling root length, root fresh weight and shoot fresh weight were the highest at 72 cells. Roughly, the more tray cells, the less se edling growth. After harvesting, among the five ports, root fresh weight and root dry weight per 1 hill of 128 cells were 0.36 g and 0.11 g, respectively, which were higher than those of other cells. Conclusion : The growth characteristics of Panax ginseng C.A. Meyer considering the seedling standing rate and seedling withering rate, among the five ports, 72 cells was most suitable. A mong the five ports, considering root fresh weight and root dry weight after harvesting, 128 c ells was most suitable.

Background : Chrysanthemum indicum L. is used in medicine, for bee, ornamental as multivoltinism plant resources. Since ancient times, Chrysanthemum indicum L. had been widely used as drug and tea. To diversity and increase the demand of Chrysanthemum, it is breeding for medicinal herb, tea and ornamental flower. Therefore size and shape of plant, color of flower have been used for cross breeding. CI2013 lines collected from Jeju have been used for landscape and ornament. and CI2010 lines collected from Sancheong have been used for medicinal herb tea. As a result, many new lines were developed. Therefore it was carried out to use as a date for new varieties through the characterization of good lines. Methods and Results : 7 lines were investigated and these plants were forstered and cultivated from Gyeongsangnam-do Agricultural Research & Extension Services (Hamyang). Breeding Lines were CI201001, CI201002, CI201005, CI201006, CI201303, CI201307, CI201401. The shape of CI201303 and CI201307 were round, plant height is low and width is wide, it may be used for landscape and ornament. As the size of flower in CI201005 was big, it may be used for medicinal herb tea. Conclusion : CI201303 and CI201307 lines were good for landscape and ornament because of round shape. CI201005 line was good for medicinal herb tea because of big flower.

Background : Cucuma longa L., in the family Zingiberaceae, is distributed in tropical and/or sub-tropical regions mainly in India and China. This species is commonly called tumeric, powder is used as medicinal herbs and/or flavor enhancer. It has been cultivated in southern region mainly Jindo. However, it might be possible to extend cultivation region due to rise in average temperature. In order to select superior lines, agronomic characteristics is commonly used. Because this is not the ultimate solution, the DNA marker approach has benefited the modern plant breeding. Therefore an easy approach by using one kind of primer have been developed from random amplification of polymorphic DNA sequences (RAPD) to discriminate effectively between different cultivars of Cucuma species Methods and Results : DNAs were extracted from the harvested roots of Cucuma sp. using DNeasy plant Mini kit (Qiagen, Hilen, Germany). These plants cultivated from GARES (Hamyang) and used for PCR amplification. The relative concentration of the extracted DNA was estimated Nano Drop ND-1000 (NanoDrop Technologies, Wklmington, De, USA) and final DNA concentration was adjusted to 5.5 ng/㎕. In this study 9 primer pairs were tested on 8 Cucuma sp. These primers showed polymorphism in Cucuma sp. The cluster dendogram showed that the similarity coefficients ranged from 0.68 to 0.87, CUR02 turned out to be CUR11, and CUR16 is similar to CUR17. Conclusion : These finding could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Cucuma species. These data on polymorphism difference based on RAPD will be give us invaluable breeding information by selection of superior lines.

Background : Teucrium veronicoides Maximowicz is a perennial plant affiliated to labiate. Ther e were many oil components such as methylchavicol, anethole, anisaldehyde, p-methoxycinnamal dehyde, 3-ocatanone and p-cymene. It has been used for indigestion, stomachache, vomit, diarrhe a in the chinese medicine field. Besides it used as medicine, it used increasingly as food additiv es, tea and air fresheners etc. This experiment was focused on plugs and planting date. That's why the plug plants are very useful for transplanting. It saves time and labor for farmer with well-management system. During the raised seeding time, they could has gotten plant health and maximum potential state. The planting date which would have something related to the growth of plug. Methods and Results : Plug plants raised in the greenhouse during 30 days. The interval of planting date was 10 days and five time planted in planting density of 60*30cm sized area with three repeat treatments. The first treatment was middle April. The last planting date was late May. Before planting period, the length of plant and the length of leaf were the highest at the middle April, respectively. The stem diameter was highest at late March. The number of leaf was the most in the late March and early April than other planting date. In the planting period, the length of plant was highest at early April. The stem diameter was also highest at that time. There were no differences on the length and the width of leaf. The number of leaf per plant was the most at the early May. The branches per plant and number of node per plant were the highest in middle April. In the respect of yield, early May had the highest dried yield than that of other treatments. It was 492.2kg/10a. The index of which was 122. The lowest was 317.2kg/10a in late May. Conclusion : About the characteristics on the growth and the yield of Teucrium veronicoides Maximowicz plug in accordance with the planting date, the yield of early May was the highest than that of other in this study. Therefore planting date of early May would have high possibility of optimum planting date.

Background : Schizonepeta tenuifolia Kitagawa is a herbaceous plant affiliated to labiate. It has many oil components which has antimicrobial activity for health with limonene, menthone, pulegon e, piperitenone, schizonepetoside A, schizonepetoside C, spatulenol. The planting area of which is com paratively small than other medicine crop as 5ha area and 15MT in the nation. The purpose of this study was to figure out the cause of characteristics on the planting life in accordance wit h the planting date used plugs. Plug plants are very useful for transplanting. It gives well-mana gement effects to the farmer such as time saving, convenience of planting, plant health, and ma ximum potential state during the raised time. Methods and Results : The number of treatment for planting date was five. Those are early May, middle May, late May, early July and middle July. The interval of the planting date was 10 days. And the raising seeding period was 30 days. During the raising seeding period, in late July treatment the length of plant and the stem diameter were the highest respectively. The number of stem was also the most in late July. In the planting period, the length of plant and the stem diameter were the highest at early May. There were no differences in the number of stem per plant. The length of ear and the width of ear were the highest at the early July. The number of ear per plant was the highest in the early May as 768.7. In the respect of yield, the weight of fresh body at early May was the highest as 587g per plant. The next higher weight of fresh body was middle May as 530.0g per plant than that of other planting date. Conclusion : The effect of planting date on the growth and the yield of Schizonepeta tenuifolia Kitagawa mainly focused to early May treatment. The earlier planting treatment was the better for planting it in this study.

Background : Senna tora L. is a herbaceous plant affiliated to legumes. It has many components good for health with emodin, chrysophanol, aloe-emodin, physicion, rhein, obtusin. It is believed to have many medicine effects, as well as to good for the eyes. This study carried to find ou t the difference of characteristics on the growth according to the planting date used plugs. Plu g plants are very useful for transplanting. It gives well-management effects to the farmer such a s time, plant health, and maximum potential state during the raised time. Methods and Results : Plug plants had 30 days in raising seeding. The planting date was five time. Fist planting date was late April. The last planting date was early July. In their raising seeding period they were taken care of in the green house. And after 30 days, they were planted on the ground in planting density of 50*40cm sized area with three repeat treatments. When the raising seeding period, the length of plant and the length of leaf were the highest at the middle May, respectively. The number of leaf per plant was highest in late May as 11. In the planting period, the length of plant was highest at early July. The lowest was the length of plant at late May. Except the stem diameter in early April, they were no differences among other treatments and the stem diameter was the highest in early April. The number of node per plant could be found the most in middle May and late April. The number of node per plant was the lowest in early July. In the respect of yield, middle May had the highest yield than that of other treatments. It was 849.0kg/10a. The lowest was 740.6kg/10a in the early May. Conclusion : About the characteristics on the growth of Senna tora L. according to the planting date, the yield of middle May was highest than that of other date in this study. The planting date of late May had also shown strong tendency of optimum planting date. Therefore middle May and late May of planting date would be optimum planting date.