Background : The Schizonepeta which is characterized by short growth period is used for the pedicel as a medicinal plant. Its main ingredients are essential oil, monoterpenoid, and flavonoid. Major effects include fever, pain, anti-inflammation, and anti-allergy. There are no varieties developed so far, and it is necessary to develop standard varieties to supply domestically good varieties. In this study, the growth and yield characteristics of the genetic resources collected for cultivating good-quality varieties were tested. Three strains were classified into early, medium, and late life. Methods and Results : The research was carried out in the test packaging of the Medicinal Resources Research Institute (Hamyang) in Gyeongnam province. It was harvested in late May, early June, and mid June by flowering season. The harvest time was mid June, late June, mid July, late July, mid August, and late August. Flowering, growth characteristics and yield were investigated. Growth characteristics showed a tendency to increase with the delay of flowering and harvesting time, such as plant height, leaf length etc. As the flowering time and harvesting time were delayed, the number of trees increased. The selected early line showed 89.4 ㎝ in plant length, 38.3 g grain weight, medium line 69.5 ㎝, 61.0 g, late line 136.4 ㎝, 111.2 g. Conclusion : When the cultivars are selected for the selected strains, it is expected that the cultivars with high physiological activity characteristics will be able to develop the cultivars as high quality varieties and high yield varieties.

Background : Rehmannia glutinosa is a perennial herb belonging to family Scrophulariaceae. This study was breeding of R. glutinosa cultivar with insect tolerance and high-yielding. Methods and Results : ‘Chunggang’ is developed by the medicinal crop breeding team of National Institute of Horticulture and Herbal Science (NIHHS), Rural Development Administration (RDA), during the period from 2005 to 2017. The reproduction of Rehmannia glutinosa has been accomplished mainly by vegetative propagation with its seedlings. This cultivar was selected from seedling of RG10. The plant type of Chungang is some rising from ground. Regional yield trials conducted at three site from 2016 to 2017. The root yield of ‘Chunggang’ was 22.3 ton per hectare, which was increased 11% compared with ‘Jihwang 1’ (check variety). Also, ‘Chunggang’ has higher insect tolerance compared with ‘Jihwang 1’. Conclusion : ‘Chunggang’ is a insect tolerance and high-yielding Rehmannia glutinosa cultivar.

Background : When the Platycodon grandiflorum is applied before the rainy season, the increase of the incidence of the Platycodon grandiflorum root rot disease increases greatly. This experiment was carried out to reduce the incidence of rot root rot disease through the lodging protecting method. Methods and Results : Three-years-old Platycodon grandiflorum was subjected to four treatments with no treatment, net installation, cutting stem, and removing bud. No treatment was used as a control, and as a further control, netting was used in the Platycodon grandiflorum to set up a treatment with almost no lodging. In the case of cutting stem, the stem was cut off in the middle of June, leaving more than 60 ㎝ before flowering. In the case of removing bud, blooming just before, the bud was removed. As a result, the coverage rate was the highest at 36.9% in the non - treatment area in the middle of July after the rainy season and 0.4% in the net installation. Compared with the case of cutting a lot of stems, 12.7% of the stem was covered with stones, whereas the stalk was 31.8%, which was close to the untreated stomach. As a result of the change of morbidity rate per treatment, it showed a morbidity rate of 49.7% in case of net installation, compared with 60% or more morbidity rate in case of untreated. Conclusion : As a result, an anti-lodging technic has helped prevent the onset of root rot disease. Further research on how to prevent the lodging of Platycodon grandiflorum using cutting stem will be needed.

Background : Turmeric is a subtropical plant, which has a high temperature for growing, but if it continues to be high temperature, it will be turned into heat degradation phenomenon. In order to mitigate damage caused by high temperature, we investigate the damage caused by high temperature. Methods and Results : This study was carried out in the experimental packaging (April 2018 seeding) of the Gyeongnam provincial medicinal resource research institute and eight systems of Turmeric (Curcuma longa) As the main research items, temperature characteristics of treatment distinction and high temperature damage rate were investigated. From July to August 2018, the main weather condition was over 35℃ for more than 21 days and showed abnormal high temperature. CUR11 showed the highest mortality rate (9.1%), and CUR10 (44.8%) showed the highest mortality rate. The average number of CUR11 strain was 23.2%. The mortality rate was 4.9% at the 60 th day of the emergence period and 40.2% at the 75 th day after 2 weeks. The higher the loss rate, the higher the damage rate. When the coating was maintained, the coating surface showed a high temperature of 50℃ or higher. A test was conducted to confirm the heat-reducing effect by coating, as a result of the test, there was no significant difference in the removal rate compared to the control. Conclusion : In order to alleviate the effect of high temperatures on the growth of turmeric, it was found that the heat loss caused by the coating did not appear much in case of turmeric.

Background : Senna tora L. is an herbaceous annual foetid herb. There were many components s uch as emodine, chrysophanol, aloe-emodine, physcion, rhein, chrysophanic acid, obtusin, aurantio-obtusin, anthraquinone compounds. The seeds and leaves are used to treat skin disease and its see ds can be utilized as a laxative, as well as to good for the eyes. This experiment was focused on the storage characteristics for searching better condition. That's why it is very useful for making oriental medicine. It is needed for Good Agricultural Practices (GAP) certification. It is one of ce rtification condition. Many medicinal plants have almost no their storage condition for GAP. Methods and Results : There are two kinds of treatment for storage test. One is temperature. The other is time period. The temperature treatments are room temperature and 4℃. Time of storage periods are four parts. The first time period for storage is zero month. The Second is until three month. and the third is until six month. The fourth is until nine month. We used polyethylene for packing materials. We investigated moisture, ash, bacteria, Escherichia coli, Staphylococcus aureus, mold and chromaticity by them. The value of moisture and ash are under standard of permission as 7.8 and 5.1 respectively. There were almost no significance about test microorganism in the storage temperature condition. There were statistical significance on the bacteria and mold without Escherichia coli, Staphylococcus aureus according to storage period. In terms of stroage temperature and period interaction, there was statistical significance on the Escherichia coli. In addition, the mycotoxin (aflatoxin) was analyzed for nine month storage Senna tora L. The total aflatoxin value were 3.307 ppb and 3.334 ppb in storage temperature and period respectively Conclusion : About the characteristics of Senna tora L. in accordance with the storage treatments, there were differences along with time period of storage about the bacteria and mold. There was no aflatoxin until nine month storage.

Background : Schizonepeta tenuifolia Kitagawa is a herbaceous plant affiliated to labiate. It ha s many oil components which has antimicrobial activity for health with limonene, menthone, p ulegone, piperitenone, schizonepetoside A, schizonepetoside C, spatulenol. The planting area of which is comparatively small than other medicine crop as 5 ha area and 15MT in the nation. This experiment was focused on the storage characteristics for searching better condition. It is very useful for making oriental medicine. It is needed for Good Agricultural Practices (GAP) certification. It is one of certification condition for farmers. Many medicinal plants have almos t been no their storage condition for GAP. Methods and Results : This experiment was carried out to conform optimal condition for S. tenuifolia Kitagawa. There were two kinds of treatment for storage test. One is temperature. The other is time period. The temperature treatments are room temperature and 4℃. The storage periods are divided into four times. Their times continued three month until nine month. There used polyethylene for packing materials. This experiment was investigated to storage condition about moisture, ash, bacteria, Escherichia coli, Staphylococcus aureus, mold and chromaticity. The contents value of moisture and ash are under standard of permission as 9.7 and 6.2 respectively. There were almost no significance about test microorganism in the storage temperature condition. There was statistical significance on the bacteria according to storage period. In terms of stroage temperature and period interaction, there was statistical significance on the Staphylococcus aureus and mold. Conclusion : The effect of storage treatments on S. tenuifolia Kitagawa mainly focused to time period treatment and time period-temperature interaction treatments, there were differences along with time period of storage about the bacteria and interaction with storage temperature and period about Staphylococcus aureus and mold.

Background : Rehmannia glutinose Liboschitz is a perennial herb belonging to the family of Scrophulariaceae. Its roots have been used as a traditional medicine. For cultivation of varieties of Rehmannia glutinose Liboschitz, the pure line selection method, which is to select the best line among those cultivated in farms for pedigree breeding, replicated yield test and regional adaptation test before registering as a new variety, is widely used. Until now, there are 8 registered varieties of Rehmannia glutinose Liboschitz in Korea. The needs for development of disaster-resistant varieties with better chances of surviving when high temperature, salts and disease are increasing. Therefore, this study focused on cultivation of disaster-resistant varieties among those selected for their disaster tolerance and salt tolerance through regional adaptation test. Methods and Results : As a result of examining the incidence of pest insects of the selected 1 - 2 years old varieties used in the study, among the 2-years-old crops, RGES 20 showed the lowest rate in ground parts. Among the 1-year-old crops, the lowest rate incidence of pest insects in ground parts was observed in RGES 23. In addition, RGES 20 and RGES 23 showed superior growth in under ground part with the highest dry root amount. Conclusion : Based on the above results, RGES 20 among the 2-years-old crops, RGES 23 among the 1-year-old crops showed superior growth in under ground part with the highest dry root amount.

Background : Panax ginseng C.A. Meyer belong to Panax species Araliaceae and a perennial plants. It grows in Far East Asia such as the Maritime province in Russia, the Manchuria reg ion of China, and the Korean peninsula. Panax ginseng C.A. Meyer is cultivated by seed sow ing or transplanting-culture, but seedling standing rate is as low as 50%. Therefore, it is impo rtant to increase seedling standing rate. This study was performed to research of plug-seedling cultivation techniques of Panax ginseng C.A. Meyer for increase of seedling standing rate. Methods and Results : Testing material, Panax ginseng C.A. Meyer seed, was sown on 5 kin ds of seedling trays (72, 105, 128, 162, 200 cells) in the middle of February 2016. It was gr own in the seedling green house in medicinal plant resources research department of Gyeongs angnam-do Agricultural Research and Extension Services. Four months after sowing, the seedli ng growth characteristics such as seedling standing rate and seedling withering rate were inves tigated. After transplanting in the mountain area of Hamyang-gun, growth characteristics of the root length, root weight, and other factors were investigated at the beginning of November. A s a result, seedling standing rates were 50.1% to 56.2% and seedling withering rates were 25. 2% to 35.9%. Among the five ports, the seedling standing rate of 105 cells and 72 cells was 56.2% and 55.7%, which were higher than others. In the case of 105 cells, however, seedling withering rate was 35.1% higher than that of 72 cells. Seedling leaf length and width were lo nger at 72, 105, 128 cells than at 162, 200 cells and seedling root length, root fresh weight and shoot fresh weight were the highest at 72 cells. Roughly, the more tray cells, the less se edling growth. After harvesting, among the five ports, root fresh weight and root dry weight per 1 hill of 128 cells were 0.36 g and 0.11 g, respectively, which were higher than those of other cells. Conclusion : The growth characteristics of Panax ginseng C.A. Meyer considering the seedling standing rate and seedling withering rate, among the five ports, 72 cells was most suitable. A mong the five ports, considering root fresh weight and root dry weight after harvesting, 128 c ells was most suitable.

Background : Chrysanthemum indicum L. is used in medicine, for bee, ornamental as multivoltinism plant resources. Since ancient times, Chrysanthemum indicum L. had been widely used as drug and tea. To diversity and increase the demand of Chrysanthemum, it is breeding for medicinal herb, tea and ornamental flower. Therefore size and shape of plant, color of flower have been used for cross breeding. CI2013 lines collected from Jeju have been used for landscape and ornament. and CI2010 lines collected from Sancheong have been used for medicinal herb tea. As a result, many new lines were developed. Therefore it was carried out to use as a date for new varieties through the characterization of good lines. Methods and Results : 7 lines were investigated and these plants were forstered and cultivated from Gyeongsangnam-do Agricultural Research & Extension Services (Hamyang). Breeding Lines were CI201001, CI201002, CI201005, CI201006, CI201303, CI201307, CI201401. The shape of CI201303 and CI201307 were round, plant height is low and width is wide, it may be used for landscape and ornament. As the size of flower in CI201005 was big, it may be used for medicinal herb tea. Conclusion : CI201303 and CI201307 lines were good for landscape and ornament because of round shape. CI201005 line was good for medicinal herb tea because of big flower.

Background : Cucuma longa L., in the family Zingiberaceae, is distributed in tropical and/or sub-tropical regions mainly in India and China. This species is commonly called tumeric, powder is used as medicinal herbs and/or flavor enhancer. It has been cultivated in southern region mainly Jindo. However, it might be possible to extend cultivation region due to rise in average temperature. In order to select superior lines, agronomic characteristics is commonly used. Because this is not the ultimate solution, the DNA marker approach has benefited the modern plant breeding. Therefore an easy approach by using one kind of primer have been developed from random amplification of polymorphic DNA sequences (RAPD) to discriminate effectively between different cultivars of Cucuma species Methods and Results : DNAs were extracted from the harvested roots of Cucuma sp. using DNeasy plant Mini kit (Qiagen, Hilen, Germany). These plants cultivated from GARES (Hamyang) and used for PCR amplification. The relative concentration of the extracted DNA was estimated Nano Drop ND-1000 (NanoDrop Technologies, Wklmington, De, USA) and final DNA concentration was adjusted to 5.5 ng/㎕. In this study 9 primer pairs were tested on 8 Cucuma sp. These primers showed polymorphism in Cucuma sp. The cluster dendogram showed that the similarity coefficients ranged from 0.68 to 0.87, CUR02 turned out to be CUR11, and CUR16 is similar to CUR17. Conclusion : These finding could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Cucuma species. These data on polymorphism difference based on RAPD will be give us invaluable breeding information by selection of superior lines.

Background : Teucrium veronicoides Maximowicz is a perennial plant affiliated to labiate. Ther e were many oil components such as methylchavicol, anethole, anisaldehyde, p-methoxycinnamal dehyde, 3-ocatanone and p-cymene. It has been used for indigestion, stomachache, vomit, diarrhe a in the chinese medicine field. Besides it used as medicine, it used increasingly as food additiv es, tea and air fresheners etc. This experiment was focused on plugs and planting date. That's why the plug plants are very useful for transplanting. It saves time and labor for farmer with well-management system. During the raised seeding time, they could has gotten plant health and maximum potential state. The planting date which would have something related to the growth of plug. Methods and Results : Plug plants raised in the greenhouse during 30 days. The interval of planting date was 10 days and five time planted in planting density of 60*30cm sized area with three repeat treatments. The first treatment was middle April. The last planting date was late May. Before planting period, the length of plant and the length of leaf were the highest at the middle April, respectively. The stem diameter was highest at late March. The number of leaf was the most in the late March and early April than other planting date. In the planting period, the length of plant was highest at early April. The stem diameter was also highest at that time. There were no differences on the length and the width of leaf. The number of leaf per plant was the most at the early May. The branches per plant and number of node per plant were the highest in middle April. In the respect of yield, early May had the highest dried yield than that of other treatments. It was 492.2kg/10a. The index of which was 122. The lowest was 317.2kg/10a in late May. Conclusion : About the characteristics on the growth and the yield of Teucrium veronicoides Maximowicz plug in accordance with the planting date, the yield of early May was the highest than that of other in this study. Therefore planting date of early May would have high possibility of optimum planting date.

Background : Schizonepeta tenuifolia Kitagawa is a herbaceous plant affiliated to labiate. It has many oil components which has antimicrobial activity for health with limonene, menthone, pulegon e, piperitenone, schizonepetoside A, schizonepetoside C, spatulenol. The planting area of which is com paratively small than other medicine crop as 5ha area and 15MT in the nation. The purpose of this study was to figure out the cause of characteristics on the planting life in accordance wit h the planting date used plugs. Plug plants are very useful for transplanting. It gives well-mana gement effects to the farmer such as time saving, convenience of planting, plant health, and ma ximum potential state during the raised time. Methods and Results : The number of treatment for planting date was five. Those are early May, middle May, late May, early July and middle July. The interval of the planting date was 10 days. And the raising seeding period was 30 days. During the raising seeding period, in late July treatment the length of plant and the stem diameter were the highest respectively. The number of stem was also the most in late July. In the planting period, the length of plant and the stem diameter were the highest at early May. There were no differences in the number of stem per plant. The length of ear and the width of ear were the highest at the early July. The number of ear per plant was the highest in the early May as 768.7. In the respect of yield, the weight of fresh body at early May was the highest as 587g per plant. The next higher weight of fresh body was middle May as 530.0g per plant than that of other planting date. Conclusion : The effect of planting date on the growth and the yield of Schizonepeta tenuifolia Kitagawa mainly focused to early May treatment. The earlier planting treatment was the better for planting it in this study.

Background : Senna tora L. is a herbaceous plant affiliated to legumes. It has many components good for health with emodin, chrysophanol, aloe-emodin, physicion, rhein, obtusin. It is believed to have many medicine effects, as well as to good for the eyes. This study carried to find ou t the difference of characteristics on the growth according to the planting date used plugs. Plu g plants are very useful for transplanting. It gives well-management effects to the farmer such a s time, plant health, and maximum potential state during the raised time. Methods and Results : Plug plants had 30 days in raising seeding. The planting date was five time. Fist planting date was late April. The last planting date was early July. In their raising seeding period they were taken care of in the green house. And after 30 days, they were planted on the ground in planting density of 50*40cm sized area with three repeat treatments. When the raising seeding period, the length of plant and the length of leaf were the highest at the middle May, respectively. The number of leaf per plant was highest in late May as 11. In the planting period, the length of plant was highest at early July. The lowest was the length of plant at late May. Except the stem diameter in early April, they were no differences among other treatments and the stem diameter was the highest in early April. The number of node per plant could be found the most in middle May and late April. The number of node per plant was the lowest in early July. In the respect of yield, middle May had the highest yield than that of other treatments. It was 849.0kg/10a. The lowest was 740.6kg/10a in the early May. Conclusion : About the characteristics on the growth of Senna tora L. according to the planting date, the yield of middle May was highest than that of other date in this study. The planting date of late May had also shown strong tendency of optimum planting date. Therefore middle May and late May of planting date would be optimum planting date.

Background : Chrysanthemum indicum L. is used in medicine, for bee, ornamental as multivoltinism plant resources. The purpose of this study was to evaluate methods of rooting promotion to make high quality medicinal plants, thereby increasing farm income. This study investigated the effects of different cuttage period, extraction site, shading degree, nursery tray for promoting cutting slips rooting in Chrysanthemum indicum L. Methods and Results : It is used New variety "Gamkuk 1" that were forstered in Gyeongsangnam-do Agricultural Research & Extension Services. Method of application with cuttage period were conducted under five condition; Around the middle of April, Toward the end of April, Early in May, Around the middle of May, Toward the end of May. Early in May plant length was longer than other cuttage period as 41.2%, 83.7% and are significant at significance level 0.05. Method of application with extraction site were conducted under three condition; 1 - 3 node, 4 - 6 node, 7 - 9 node. Leaf number in 1 - 3 node was more than other extration site as 19.4%, 33.6%. Also root length in 1 - 3 was more than other extration site as 10.5%, 23.2%. Method of application with shading degree were conducted under three condition; 50%, 70%, 90%. Plant length in 50 - 70% was longer than 90% shading degree as 23.8%, 24.2%. Also shading degree 50 - 70% had many root length and root number. Method of application with nursery tray were conducted under five condition; 72, 105, 128, 162, 200 tray. Plant length in 105, 128 tray was longer as 8.5 - 35.3% than other nursery tray and are significant at significance level 0.05. Conclusion : According to the results, Early in May, extraction site 1 - 3 node, shading degree 50 - 70%, nusery tray 105, 128 showed the highest growth on cutting slips root promotion in Chrysanthemum indicum L.