본고에서는 최근 중국을 중심으로 추진되고 있는 한약재 ISO 국제표준화에 대한 대응을 위해 최신 등재 현황을 소개하고 주요 추진상의 문제점과 대응방안들을 점검해 보았다. 주요 내용을 정리하면 다음과 같다. 1. 국제표준화기구 전통의학 기술위원회(ISO TC249)는 2009년 설립이후 31개의 국가가 참여하여 한약재 국제표준화를 추진하고 있다. 한·중·일은 발의 문건은 90%이상을 차지하여 사실상 아시아의 전통의학 분야를 두고 경쟁하고 있으며 간사국인 중국은 약용작물의 종자 ·종묘에서부터 약재의 생산, 품질 그리고 침구, 의료기술 등에 이르기까지 전 한방분야에서 표준화를 주도하고 있다. 현재 진행되고 있는 약용작물 재배 및 한약재 품질 관련 안건만 약 50여건에 달해 향후 5년 내에 시장 거래량 상위품목들의 국제표준화 작업이 대부분 완료될 것으로 예상된다. 2. 현재 진행되고 있는 안건들은 한약재의 품질이나 품질평가에 관한 안건들이 대부분이다. 이 안건들이 국제표준으로 인정되면 세계 한약재 유통시장에는 새로운 질서와 우열기준이 생기고 유통 한약재의 시장가치 평가에 반영되어 각국 한약재 점유율 변동에도 영향을 줄 수 있다. 이들 안건들은 직접적인 재배생산 표준이 아닌 경우가 대부분이지만 성분이나 성상 등에 대한 표준을 담고 있으므로 대부분 재배생산과도 밀접한 관련이 있다. 만일 중국 중심의 표준화가 계속해서 심화된다면 장기적으로는 국내 생산농가에 부담을 줄 수도 있다. 그러므로 한약재 국제표준화 대응은 향후 국내 생산물이 표준규격을 달성할 수 있는 범위내로 기준을 유도하는 전략을 펴야 할 것이다. 이를 위해 국내 생산물의 특성을 파악하고 선반영 하려는 노력이 필요하다. 3. 한약재 표준화 대응은 중국의 추진안건이 많으므로 분야별 파급영향을 고려한 선별적인 대응이 필요하다. 종자·종묘 등 추가적인 제한 필요성이 낮은 분야보다는 향후 시장에서 한· 중 간 경쟁이 발생할 수 있는 분야에 집중하는 것이 중요하다 . 원산지 변조, 농약 잔류량·이산화황 과다검출 등의 이력이 있는 약재들은 해당 표준을 강화하고 재배환경의 영향을 받을 수 있는 도지약재들의 경우 성상이나 지표성분 등에 대한 국내약재의 특성을 최대한 반영할 필요성이 있다. 곰팡이나 해충관리 등은 아직 각국의 관리규정이나 저온저장 시설 등 현장 인프라 구축이 충분치 않은 점들도 고려되어야 한다. 아직 국제표준을 추진하기 위한 국내의 연구결과들이 충분치 않은 부분들이 많으므로 추가적인 연구와 지원이 필요할 것이다.

This study pointed out problems on the operation by estimating process efficiency to the 3N-system(quick fermentable treatment process) which occupy plant to treat swine wastewater more than about 30 % in Jeju. And the parts to improve from problems were deduced and introduced those in situ. When the parts to improve from problems were not introduced, the Concentration of H₂S and NH₄ was 5 and 50ppm in the fermenter, respectively. But the concentration of H₂S and NH₃ was shown N.D. and 1~2 ppm in the fermenter after introduction. The concentration of H₂S and NH₃ were measured by 16 and 300 ppm highly in the aeratin tank before improvement, but those were shown N.D. and 0~1 ppm after improvement, respectively. Therefore, it showed possibility which could decrease odor efficiently with low cost by this study in 3N-system.

본 연구는 지황의 플러그 육묘 재배가 생산량을 증가시킬 수 있는지 평가하고, 강원도 남부지역에 플러그 육묘를 이용한 지황 재배 시 중부지역의 직파재배만큼 생산량을 늘릴 수 있는지 알아보기 위해 수행하였다. 수량의 경우 음성 육묘재배(1,376 ㎏/10a)가 가장 높았고, 그 다음으로 평창 육묘재배(1,256 ㎏ /10a), 음성 직파재배(1,253 ㎏/10a), 평창 직파재배(923 ㎏ /10a) 순으로 나타나, 강원도에서 육묘재배시에 중부지역 직파 재배만큼 생산량을 늘릴 수 있는 것으로 나타났다. 또한, 지황은 근경 굵기가 8-12 ㎜가 중품(中品), 그 이상이 상품(上品)의 등급으로 팔리는데, 재배실험지역과 재배방법에 상관없이 모두 14 ㎜가 넘어 상품성(商品性)에도 문제가 없었다. 그러므로 생산량과 품질 조건을 고려해 볼 때, 플러그 육묘 재배를 통해 생산량을 늘릴 수 있었으며, 강원도에서 플러그 육묘를 이용한 지황 재배 시 중부지방의 직파재배와 비슷한 생산이 가능할 것으로 생각된다. 그러나 본 연구결과를 적용하기에는 늘어나는 생산비와 증가되는 수익을 고려한 경제성 분석이 더 필요할 것으로 생각된다.

본 연구는 지황이 강원도 남부지역에서 재배가 가능한 대체 작목인지 평가하기 위해 수행하였다. 수량의 경우 음성 1,848 ㎏/10a, 정선 1,571 ㎏/10a, 평창 1,485 ㎏/10a로, 2015년부터 2017년 지황 평균 단수인 838 ㎏/10a보다 높게 나타났다. 또한, 지황은 근경 굵기가 15 ㎜ 전후가 되면 상품(上品)의 등급으로 팔리는데, 강원도 재배지역 모두 15 ㎜가 넘어 상품성(商品性) 에도 문제가 없었다. 그러므로 생산량과 품질 조건을 고려해 볼 때, 현재 강원도에서 지황 재배는 가능할 것으로 생각된다. 그러나 본 연구결과를 적용하기에는 강원도의 여러 제반 사항을 고려한 경제성 분석이 더 필요할 것으로 생각된다.

본 연구는 참당귀 재배 시에 발생하는 추대 참당귀를 부산물로 활용가능성이 있는지 평가하기 위해 수행하였다. 추대 참당귀의 지상부와 지하부의 총 무게는 수확기 비추대 참당귀의 지하부 무게 대비 약 98.5%로 높게 나타났다. 또한 추대 당귀의 decursin과 decursinol angelate의 총 함량은 주 생산품인 참당귀 신(身)의 지표성분 총 함량인 4.24%와 대비해 부위별로 0.64%~2.39%로 나타났으며, 이는 추출물로 이용 시 생산품 대비 15~56%에 해당하는 것으로 나타났다. 또한, 항상화 활성의 경우 추대 참당귀는 전반적으로 비추대 참당귀에 비해 우수한 것으로 나타났다. 추대 참당귀의 생산량, 지표성분 함량 그리고 항산화 활성 결과를 고려해 봤을 때, 추대 참당귀의 부산물은 사료 첨가제 및 화장품 용도의 추출물로 이용 가능성이 높은 것으로 생각된다.

Angelica gigas Nakai (AGN) is a traditional medicinal herb especially in Korea. It contains pyranocoumarins, which are major active components including decursin (D) and decursinol angelate (DA). This study was carried out to determine the change in active component content and antioxidant activity depending on the root diameter of AGN. Several processing steps are involved to use AGN roots as medicine. The dried AGN roots are divided into body (B), thick root (TkR), medium root (MR) and thin root (TnR) according to their diameter before cutting into medicine. The recovery rates of each root parts per 100 ㎏ were measured as 32.3±2.5, 9.0±1.0, 39.3±2.1 and 15.0±1.0%, respectively and the mean diameters were measured as 51.95±4.55, 7.05±0.89, 2.88±0.49 and 1.57±0.32 ㎜. Two index components, D and DA, were analyzed. The change of both D and DA content showed a similar tendency. Both D and DA content were increased as the root diameter decreased (higher in TnR). In addition, antioxidant activity was higher in B and TnR, and lower in TkR and MR than control. This study showed that the thinner the root diameter, the higher the D and DA content in AGN roots and that TnR has excellent antioxidant activity compared to other root parts, suggesting that the thinner root part of AGN could be used as a potential material.

Background: Astragalus membranaceus belonging to the Leguminosae family is often utilized as a traditional medicine. The aim of this study was to elucidate the basic breeding information required to develop short stem A. membranaceus cultivars. Methods and Results: Roots of A. membranaceus advanced yield trial (AYT) lines were harvested in late October 2017. Root yield of six AYT lines were increased in a range of 8.9 - 74.8% compared with ‘Aseong’ as control (check variety). The height of seven AYT lines were shorter than that of ‘Aseong’. In addition, stem diameter of nine AYT lines was thicker than that of ‘Aseong’. Consequently, 1502-56, 1503-90, and 1510-80 were selected as elite lines for the development of short stem cultivars. HPLC analysis was perfromed to identify lines with high level active components such as calycosin-7-O-β-D-glucoside and calycosin. The levels of both active components were higher in 1502-56, and 1503-90, but not in 1510-80 compared to ‘Aseong’. In addition, 2,2-Diphenyl- 1-picryl hydrazyl (DPPH) radical scavenging activity was higher in the 1502-56 compared to ‘Aseong’. Considering these results, two AYT lines, 1502-56 and 1503-90 were selected as short stem lines with high calycosin-7-O-β-D-glucoside and calycosin content. Conclusions: Taken together, Two short stem lines were identified in this study. In our future study, regional yield trial (RYT) will be conducted using these selected lines to develop new cultivars.

Background : Adenophora triphylla var japonica is a perennial herb that belongs to Campanulaceae. Radix Adenophorae is a dried rhizome of A. triphylla and same genus plant. It has contains chemicals such as cycloartenyl acetate, lupenone, β-sitosterol, taraxerone, octacosanoic acid, and praeruptorin A. Radix Adenophorae considered to be effective regulating humoral and cellular immunity, antimutation, restraining adenocarcinoma cell, strengthening cardiac function, allaying a fever, and easing pain and cough. In this study, we tried to establish a mass production system of A. triphylla which has high economic value as a medicinal herb by plant tissue culture in order to cultivate standard varieties. Methods and Results : In this study, A. triphylla internode was used as a explant and it was surface sterilized by 1% sodium hypochlorite for 5 minutes, consequently several times washed with ddH2O. Further it was placed in to MS medium including with axillary bud. The 1/2MS, B5, SH was used in this research. And the plant growth regulator of 0.1 - 2 ㎎/ℓ auxins (NAA, IBA) and cytokines (BA) were used respectively to achieve multiple shoots. The whole study was carried out in the department of Herbal crop research, Eumseong, RDA. Conclusion : In this study we obtained, 6.2 multi-shoots per an explant, and the shoot growth was also favorable in the presence of 1.0 ㎎/ℓ BA and 1.0 ㎎/ℓ IBA.

Background : Ligusticum chuanxiong Hort is a perennial herb of the Umbelliferae family and an important traditional oriental medicinal plant. The compounds contained in L. chuanxiong can be divided into five kinds, essential oil, alkaloids, phenolic acids, phthalide lactones, and other constituents. These compounds have cardiovascular and cerebrovascular effects, antioxidants, neuroprotection, anti-fibrinolytic, antidotes, anti-inflammatory and antitumor activities. In this study, we anticipated to establish the in vitro propagation system of L. chuanxiong, which is a high economic value as medicinal herb, by plant tissue culture to solve the problem of root stocks contamination. Methods and Results : The whole study was carried out in the department of Herbal crop research, Eumseong, RDA. In this study, L. chuanxiong nodes was used as an explant and it was surface sterilized by 2% sodium hypochlorite for 1 minutes, then washed with ddH2O several times. Further the surface sterilized nodes were placed on MS basal media. Multiple shoots were induced on MS, SH, WPM media with 0.1 - 2 ㎎/ℓ auxin (NAA, IBA) and cytokine (BA). In this study we obtained 4.6 multi-shoots per an explant, and growth of the shoot was also favorable in the presence of 1.0 ㎎/ℓ BA. Subsequent transfer of these regenerated shoots on 1/2 MS media resulted in root formation. The rooted plantlets were able to grow in soil after 3 weeks of acclimatization. Conclusion : The optimal conditions for in vitro propagation of L. chuanxiong were established through this experiment.

Background : Astragalus membranaceus belonging to the family of Leguminosae have been utilized as a traditional medicine. The aim of this study is to elucidate the basic information for breeding to superior Astragalus membranaceus cultivar. Methods and Results : Selection lines were developed by the medicinal crop breeding team of National Institute of Horticulture and Herbal Science (NIHHS), Rural Development Administration (RDA). The root of A. membranaceus lines were harvested in late October 2017. Calycosin-7-O-β-d-glucoside and calycosin were anlyzed by HPLC. The root yield of six selection lines were increased 8.9 - 74.8% compared with ‘Aseong’ (check variety). Also, the plant height of seven selection lines were shorter than ‘Aseong’. The calycosin–7-O-β -d-glucoside content was higher in six selection lines than ‘Aseong’. The calycosin content was higher in all selection lines except 1 line (1508 - 03) than that of ‘Aseong’. Conclusion : Three superior lines with short height and high yield were selected. These superior lines will conduct advanced yield trial to make varieties.

Background : Rehmannia glutinosa is a perennial herb belonging to family Scrophulariaceae. This study was breeding of R. glutinosa cultivar with insect tolerance and high-yielding. Methods and Results : ‘Chunggang’ is developed by the medicinal crop breeding team of National Institute of Horticulture and Herbal Science (NIHHS), Rural Development Administration (RDA), during the period from 2005 to 2017. The reproduction of Rehmannia glutinosa has been accomplished mainly by vegetative propagation with its seedlings. This cultivar was selected from seedling of RG10. The plant type of Chungang is some rising from ground. Regional yield trials conducted at three site from 2016 to 2017. The root yield of ‘Chunggang’ was 22.3 ton per hectare, which was increased 11% compared with ‘Jihwang 1’ (check variety). Also, ‘Chunggang’ has higher insect tolerance compared with ‘Jihwang 1’. Conclusion : ‘Chunggang’ is a insect tolerance and high-yielding Rehmannia glutinosa cultivar.

Background : Rehmannia glutinosa is a perennial herb belonging to family Scrophulariaceae. This study was conducted to evaluate the appropriateness through seedling cultivation of R. glutinosa. Methods and Results : The root of R. glutinosa cultivar (Jihwang 1) was harvested in the middle of March. Seed rhizomes were seedling after air curing for three days. And seed rhizomes were plug seedlings for 30 days (30D), 45 days (45D) and 60 days (60D) then planted in the test field. Mat formation rate in 30D, 45D and 60D was 81.3, 100 and 100% respectively. Flowering occurred only in 45D and 60D except 30D. Flowering rate of 45D and 60D were 7.3 ± 3.06 and 13.3 ± 1.15%. Agronomic characteristics were evaluated in comparison with ND (directly seeded group). As a results, aerial parts of 30D in plant height, leaf length and leaf width were better than other treatment group but the number of leaf per plant was statistically the same. Also, root shape was similar when comparing ND and seedling groups. Conclusion : Taken together, it is thought that the cultivation of R. glutinosa. seedlings is possible. However, further evaluation of the economic aspect is needed.

The use of Rehmannia glutinosa of the family Scrophulariaceae, in traditional medicine is well known. This study was conducted to elucidate the effect of air curing of the seed rhizome of R. glutinosa on its storability and yield. Methods and Results: The root of the R. glutinosa cultivar (Dagang) was harvested in at the end of November. The seed rhizomes were air cured for one to seven days. They were subsequently wrapped with newspaper and further stored in a plastic container at 1℃. It was observed that the weight loss and decay rates were significantly lower in the air cured seeds than in the untreated ones. Moreover, the decay rate of the control was approximately 50%, 120 days after storage. However. the decay rate of all the air cured treatment groups was less than 1%. Additionally, air curing led to an increase in the germination rate of the seeds and the root yield when compared with the untreated groups. Taken together, the ideal treatment period for air curing was found to be 3 days. Under these conditions, the germination rate and yield were 88.7% and 2,185 ㎏/10a, respectively. Conclusions: This study successfully demonstrated that the air curing of seed rhizomes can lead to a considerable increase in the storability and yield of R. glutinosa.

Background : Angelica gigas Nakai (AGN) is a perennial plant found in East Asian countries and an crucial medicinal herb especially in Korea. It contains several chemicals like pyranocoumarins, which are major active components including decursin (D) and decursinol angelate (DA), essential oils and polyacetylenes. This study was carried out to determine the change in active components depending on the root diameter of AGN. Methods and Results : Several processing steps are involved to use AGN roots as medicine. The dried AGN roots are divided into body (B), thick root (TkR), medium root (MR) and thin root (TnR) according to their diameter before cutting into medicine. Among them, B and TkR are mainly used as medicine. The mean diameter of dried each root part (B, TkR, MR, TnR) were measured as 51.95, 7.05, 2.88, and 1.57 ㎜, respectively. The water content of each root part was slightly reduced depending on the size of the diameter (higher in B). In addition, the two active components, D and DA, were analyzed by high performance liquid chromatography. The change of both D and DA content showed a similar tendency. Both D and DA content were increased as the root diameter decreased (higher in TnR). Conclusion : This study showed that the thinner the root diameter, the higher the D and DA content in AGN roots, suggesting that the thinner root parts of AGN could be used as potential materials.

Background : Platycodon grandiflorum has been used as famous medicinal vegetable and traditional medicine in Korea. Platycodon grandiflorum is used more for vegetable than it is used as a medicine. Proper storage conditons are required for distribution of Platycodon grandiflorum as vegetables. The purpose of this study was to find the appropriate storage temperature. Methods and Results : 2-years old Platycodon grandiflorum cultivated in Herbal Crop Research, RDA Eumseong was used. They were packed with plastic container box of 45 × 70 × 30 ㎝ and then stored at –2℃, 2℃, 5℃, 25℃, humidity 80 - 82%. During the storage, hardness, L a b values, weight loss, decay rate were measured every 30 days as quality indices. During the storage period, L value decreased but a and b values increased and same pattern was observed at all storage temperatures. Hardness decreased and same pattern was also observed at all storage temperature. Under constant humidity conditions, weight loss was larger at higher temperature. The higher the temperature, the larger the deviation at weight loss. There were little decay but 25℃ temperature stored showed that dacay rate was higher with time over. Conclusion : The favorable temperature for storage is –2℃ - 2℃ while maintaining constant humidity.