Cnidium officinale M. is an important crop that is widely used as a raw material for health functional foods. However, it is experiencing cultivation difficulties due to climate change and abnormally high temperatures. In response to this problem, the characteristics and main causes of the high-temperature damage occurring in C. officinale M. cultivation fields were analyzed. A survey of five farmhouse fields in Jecheon and Bonghwa, major C. officinale M. cultivation areas in Korea in 2018, indicated that about 5% to 37% of the cultivation fields in Jecheon and 5% to 15% of the fields in Bonghwa died from wilting. The high-temperature damage of the C. officinale M. fields is divided into two categories: upper leaves drying due to solar radiation and temperature, and lower leaves dying serially to the radiant heat of the vinyl mulch. Damage caused by radiant heat was typically greater. This is due to the greenhouse effect that occurs in the small space between the black vinyl mulching and the soil. The heat radiated to the surface of the ridge creating an environmental condition that greatly exceeded the atmospheric temperature especially on hot days. As a result, short plants with underground parts, such as C. officinale M., can suffer more high-temperature damage than other plants, so it is considered that it is necessary to develop related technologies such as mulching materials that can reduce pavement temperature in the future.

As carbon fibers have the high performance, such as high strength, light weight, and high corrosion resistance, they are gradually expanding and growing at various industries. In particular, this study was conducted to determine whether the market could be applied to street lanterns among the construction sectors expected to grow in scale. In this study, the material characteristics of street lanterns using carbon fiber composites were studied in order to determine whether carbon fiber composites could be applied as a substitute for stainless steel and cast iron street lanterns. The physical relationship was experimentally evaluated to obtain a suitable material through the comparison of physical properties of fiber composite materials.

본고에서는 최근 중국을 중심으로 추진되고 있는 한약재 ISO 국제표준화에 대한 대응을 위해 최신 등재 현황을 소개하고 주요 추진상의 문제점과 대응방안들을 점검해 보았다. 주요 내용을 정리하면 다음과 같다. 1. 국제표준화기구 전통의학 기술위원회(ISO TC249)는 2009년 설립이후 31개의 국가가 참여하여 한약재 국제표준화를 추진하고 있다. 한·중·일은 발의 문건은 90%이상을 차지하여 사실상 아시아의 전통의학 분야를 두고 경쟁하고 있으며 간사국인 중국은 약용작물의 종자 ·종묘에서부터 약재의 생산, 품질 그리고 침구, 의료기술 등에 이르기까지 전 한방분야에서 표준화를 주도하고 있다. 현재 진행되고 있는 약용작물 재배 및 한약재 품질 관련 안건만 약 50여건에 달해 향후 5년 내에 시장 거래량 상위품목들의 국제표준화 작업이 대부분 완료될 것으로 예상된다. 2. 현재 진행되고 있는 안건들은 한약재의 품질이나 품질평가에 관한 안건들이 대부분이다. 이 안건들이 국제표준으로 인정되면 세계 한약재 유통시장에는 새로운 질서와 우열기준이 생기고 유통 한약재의 시장가치 평가에 반영되어 각국 한약재 점유율 변동에도 영향을 줄 수 있다. 이들 안건들은 직접적인 재배생산 표준이 아닌 경우가 대부분이지만 성분이나 성상 등에 대한 표준을 담고 있으므로 대부분 재배생산과도 밀접한 관련이 있다. 만일 중국 중심의 표준화가 계속해서 심화된다면 장기적으로는 국내 생산농가에 부담을 줄 수도 있다. 그러므로 한약재 국제표준화 대응은 향후 국내 생산물이 표준규격을 달성할 수 있는 범위내로 기준을 유도하는 전략을 펴야 할 것이다. 이를 위해 국내 생산물의 특성을 파악하고 선반영 하려는 노력이 필요하다. 3. 한약재 표준화 대응은 중국의 추진안건이 많으므로 분야별 파급영향을 고려한 선별적인 대응이 필요하다. 종자·종묘 등 추가적인 제한 필요성이 낮은 분야보다는 향후 시장에서 한· 중 간 경쟁이 발생할 수 있는 분야에 집중하는 것이 중요하다 . 원산지 변조, 농약 잔류량·이산화황 과다검출 등의 이력이 있는 약재들은 해당 표준을 강화하고 재배환경의 영향을 받을 수 있는 도지약재들의 경우 성상이나 지표성분 등에 대한 국내약재의 특성을 최대한 반영할 필요성이 있다. 곰팡이나 해충관리 등은 아직 각국의 관리규정이나 저온저장 시설 등 현장 인프라 구축이 충분치 않은 점들도 고려되어야 한다. 아직 국제표준을 추진하기 위한 국내의 연구결과들이 충분치 않은 부분들이 많으므로 추가적인 연구와 지원이 필요할 것이다.

한약재는 저장 및 유통과정에서 약 20여종의 저장해충이 발생하는 것으로 알려져 있으나 화랑곡나방(Plodia interpunctella H.), 권연벌레(Lasioderma serricorne F.), 거짓쌀도둑거저리(Tribolium castaneum H.) 등 일부 우점해충이 이들 피해의 절반이상을 차지하고 있는 것으로 파악되고 있다. 그런데 시중유통 한약재 중에는 저장해충 외에 권련침벌 등 포식성 해충이 함께 발견되고 있으나 이에 대한 조사나 연구결과는 거의 없는 상황이다. 본 연구는 한약재 저장해충의 생태네트워크를 파악하기 위하여 기존의 문헌들을 바탕으로 이들 해충의 포식관계를 조사하고 시중유통 한약재 중 비교적 해충오염도가 높은 약재를 번식이 유리한 온습도 조건에서 보관하며 해충밀도를 높인 후 자연 증식한 숙주와 포식해충의 생태를 현미경으로 관찰하였다. 국내외 문헌조사결과 권연벌레의 천적은 권련침벌, 곤충병원성선충, 쌀바구미금좀벌, 긴털가루응애 등이 밝혀진바 있고, 화랑곡나방의 천적은 보리나방살이고치벌이 그리고 거짓쌀도둑거저리의 천적은 긴털가루응애(Tyrophagus putrescentiae S.)가 확인된바 있다. 실제로 오염 한약재인 천궁, 방풍 등을 보관하는 과정에서도 권연벌레의 밀도가 높아지자 권련침벌(Cephalonomia gallicola H.)이 크게 발생하였고, 긴털가루응애는 권연벌레와 거짓쌀도둑거저리 모두에게서 발견되었다. 그러나 권련침벌의 경우 한약재를 구입할 때부터 포장지 내에 권연벌레와 함께 있는 것이 확인되었으나 긴털가루응애는 처음부터 한약재 포장 속에 있었는지 한약재 보관실험과정에서 주변으로부터 오염되었는지는 확인되지 않았다. 긴털가루응애의 발생직후 확산속도는 습도가 높을 경우 권련침벌보다 훨씬 빨랐으며 습도를 30% 이하로 낮출 경우에는 밀도가 크게 떨어졌다. Sm. Ch. Papadopoulou(2006)는 권련침벌이 한집단내 권연벌레 유충을 평균 약 20%가량 포식하는 것으로 조사하였으나 본 관찰결과 이보다 훨씬 많이 발생하였으며 권연벌레의 밀도를 90%이상 감소시킨 경우도 있었다. 본 조사를 통해 관련 천적들이 주요 저장해충의 밀도를 어떤 조건에서 얼마나 낮출 수 있는지에 관한 계량적인 연구결과는 아직 부족하나 천적으로 활용하기에는 인체 위해성 및 다른 식품 등으로의 2차오염 가능성이 있어 다소 부적합 할 것으로 판단된다.

The present study aimed to verify the effects of DFO on PDL cells, with particular emphasis on focusing on osteoblastic differentiation. Its mechanisms related to heme oxygenase-1 (HO-1) pathway were also analyzed. DFO increased the expression of HO-1 and early osteoblastic differentiation markers, such as alkaline phosphatase (ALP) and bone sialoprotein (BSP). DFO upregulated heme oxygenase-1. Treatment with HO-1 siRNA blocked the DFO-stimulated osteoblastic differentiation and HO-1 expression. The NF-kB inhibitor pyrrolidine dithiocarbamate, phosphatidylinositol 3-kinase inhibitor Wortmannin, and p38 MAPK inhibitor U0126 blocked the effects of DFO on HO-1 expression and osteoblastic differentiation in PDL cells. Collectively, these data suggest that DFO promotes osteoblastic differentiation and induces the expression of defense protein HO-1 probably via PI3K, p38 MAPK, and NF-kB signalling pathways in PDL cells.

Eukaryotic translation initiation factor 5A (eIF-5A) is essential for proliferation of eukaryotic cells, andwas identified as diagnotic marker in cervical intraepithelial neoplasia, cervical and endometrial cancer, but relatively little is known about thein vivo and in vitro expression patterns of eIF-5A in oral premalignant and malignant lesions mirror the expression levels observed in vitro with cells derived from normal oral mucosa, immortalized oral keratinocytes (IHOK) and primary and metastatic oral squamous cell carcinoma (OSCC). We used an oral squamous cell carcinoma (OSCC) progression model composed of cell lines and tissue specimens to characterize expression patterns by Western blotting and immunohistochemistry. eIF-5A and PCNA levels are elevated in IHOKand primary and metastatic OSCC cella as compatred to normal human oral keraitinocytes. eIF5A and PCNA expression was l imited to basal cells of normal oral mucosa. eIF-5A and PCNA expression is increased in dysplastic epithelium spreading to more superficial layers, and its expression levels correlated significantly with the degree of dysplasia. Well and moderately differentiated OSCC showed strong expression of eIF-5A and PCNA. These results suggest that upregulated expression of eIF-5A seems to be an important epigenetic alteration that accompanies oral carcinogenic progression, and eIF-5A could be used as an biomarker for oral premalignat lesion or squamous cell carcinoma

ECR-PECVD법을 사용하여 450-490˚C이하의 온도에서 Pt/SiO2/Si기판 위에 PZT 박막을 증착하였다. 기판 온도가 460˚C 이하일 경우에는 페로브스이트 상과 제2상으로 이루어진 박막이 성장하였으며 기판온도가 470˚C이상일 때에는 페로브스카이트 단일상의 PZT 박막이 성장하였다. 490˚C에서 매우 얇은 페로브스카이트의 PZT 박막을 증착한 후 650˚C에서 1분간 raped thermal annealing(RTA) 처리한 결과 박막의 조성과 결정성에는 거의 변화가 없었으나 박막의 전하 저장 밀도는 크게 향상되었다. 이는 RTA 처리에 의한 저유전 계면층의 소멸이 주된 이유라고 판단된다. 열처리 후 두께 40-45nm의 PZT박막은 200kV/cm의 전장 하에서 10-6cm2이하의 누설전류값을 갖고 있었으며, 인가전압 1V에서 300fF/μm2의 정전용량, 즉 SiO2환산두께 0.12nm를 나타내었다.

본 실험은 일당귀의 개화 후 일수에 따른 종자 등숙 특성을 알아보고자 수행하였다. 2019년 농촌진흥청 약용작물과 시험 포장에서 채종한 종자를 시험재료로 사용하였다. 개화 후 일수에 따라 종자 무게와 발아율이 조사되었고, 등숙 과정 동안 종자 내에서 배종비(E:S ratio)가 측정되었다. 결과적으로는 각 소화서마다 개화 후 일수가 증가할수록 종자 무게가 유의적으로 증가하였으며, 각 소화서에서 발아가 시작되는 시기는 차이가 있었다. 또한 종자 내에서 배의 길이는 계속해서 성장하여 배종비가 높아지는 것을 관찰하였다. 일당귀는 다양한 소화서에서 꽃이 피기 때문에 종자의 배종비가 종자 발아에 영향을 미칠 수 있다. 본 연구를 바탕으로 일당귀의 우량 종자 생산을 위해서는 개화 후 50일부터 70일경이 가장 적합한 것으로 사료된다.

Background : In previous study, we reported Sclerotium rot caused by Sclerotium rolfsii in Ixeridium dentatum for the first time. This experiment was conducted to select highly effective pesticides against Sclerotium rot caused by S. rolfsii in I. dentatum. Methods and Results : The chemical efficacy and the injury test were carried out. A total of five pesticides were used for the experiment test. For the efficacy test, we investigated spore germination and mycelial growth inhibiting ability by each pesticides in vitro and disease inhibiting ability in the field. For the chemical injury, we investigated appearance of abnormalities on condition of reference amount and fold amount in the field. In vitro, three kinds of chemicals such as Fludioxonil suspension concentrate (SC), Tebuconazole suspension concentrate (SC), and Flutolanil emulsifiable concentrate (EC) showed complete spore germination inhibitory effect, However in two chemicals such as Pyraclostrobin water-dispersible granule (WG) and Pyribencarb suspension concentrate (SC), the mycelial growth inhibitory effect was partially recognized but the spore germination was not inhibited. In the field, we performed an artificial inoculation experiment using sclerotia. As a result four kinds of chemicals such as Fludioxonil SC, Tebuconazole SC, Flutolanil EC, and Pyraclostrobin WG showed control value of above 80% against Sclerotium rot caused by Sclerotium rolfsii except Pyribencarb SC. Also there was no chemical injury in reference amount and in fold amount respectively, compared to non treated control. Conclusion : From the above results, we selected four items of pesticides including Fludioxonil SC, Tebuconazole SC, Flutolanil EC, and Pyraclostrobin WG as effective chemicals against Sclerotium rot caused by Sclerotium rolfsii in Ixeridium dentatum.

Background : In the process of developing new disease-resistant cultivar of Rehmannia glutinosa, it is essential to screen plants based on resistance to their disease. But until now, we have relied on visual inspection for selection of disease-resistant cultivar. Therefore, this experiment was carried out to establish an efficient and reliable screening system and test the resistance to leaf spot disease in R. glutinosa cultivars developed until now. Methods and Results : We have tested 11 R. glutinosa cultivars developed so far. Rootstock of R. glutinosa were sown in 20 × 20 ㎝ plastic square port and grown in green house at 25 ± 5℃ for four months and then cutting leaves were inoculated with Phoma sp. HCRD 17103 by spraying spore suspention of fungus at concentration of 1 × 107 spore/㎖. The inoculated leaves were incubated in a dew chamber at 25℃ for 48h and then transferred to glass house (25 ± 5℃, RH 80% ≥ ). After 3 - 5 days when the most susceptible cultivar had lesion area over 40%, disease severity of the cultivars was investigated on a scale of 0 - 9 (0 = healthy, 1 = 0 - 1%, 3 = 1.1 - 10%, 5 = 10.1 - 20%, 7 = 20.1 - 40%, 9 = 40.1% over). The degree of resistance was determined according to the disease index (0 and 1 = resistant, 3 = moderately resistant, 5, 7 and 9 = susceptible). As a result of experiments according to the above criteria, the disease index of cultivar Gogang, Dagang, Segang, Yeongang, Wongang, and Dahwang was 1, cultivar Togang, Daegyung, Jiwhang 1 and Goryeo was 3, cultivar Hwanggang was 9, respectively. Conclusion : From the above results, six cultivars such as Gogang, Dagang, Segang, Yeongang, Wongang, and Dahwang were resistant, four cultivar such as Togang, Daegyung, Jiwhang 1 and Goryeo were moderately resistant, and only one cultivar Hwanggang was susceptible to the leaf spot disease by Phoma sp. This experiment used only leaves and we plan to use whole plants in the future to see more accurate resistance response.

Background : This study was conducted to investigate the production competitiveness of medicinal crop. The results of this study were intended to be used as basic data for establishing the direction of R&D needed for domestic medicinal crop farming system. Methods and Results : For data analysis, frequency, percentage and average and Chi square (× 2) value were used. The survey showed that the cultivated crops of the respondents were medicinal crop. Firstly, there was a significant difference in farming disability by farming career. The 'cultivation method (60%, 42.1%)' was high in farming preparer and beginner (≤ 3 years), but the disability in 'cultivation method' decreased as the farming career was longer. Respondents who had more than 10 years of farming career complained of 'climate problem (24.0%)' followed by 'cultivation method (20.0%)' and 'pest control (20.0%)'. There was also a significant difference in the farming disability by medicinal crops farming career. The 'cultivation method (50.0%)' was the highest of the farmers who had 1 year of medicinal crops farming career, however the respondents with more than 4-ears of career complained of 'climate problems (27.3%)' and 'pest control (23.6%)', but 'cultivation method' was low as 18.2%. Secondly, there was a difference in sales disability by farming career, and cross-sectional analysis was statistically significant at × 2 = 41.320. The respondents who were preparer for farming had the biggest sales disability at 'shortage of market (44.4%)', and the rates decreased gradually as the farming career increased. Respondents more than 10-ears had the biggest sales disability as ‘uncertain market price (50.0%)’, and 'shortage of market' was low as 12.5%. Cross-sectional analysis of sales disability by medicinal crops farming career showed that × 2 was 49.705, which was statistically significant. Farmers with no career in cultivating medicinal crops had the biggest sales disability at 'shortage of market (40.0%)' and farmers with more than 4 years of career complained of 'uncertain market price (42.2%)'. Lastly, there was a statistically significant difference in cultivation performance by medicinal crops farming career. The respondents with a career of less than 1 year had the highest proportion at 'medium (48.5%)', but those with more than 4-ears of career had the highest rate of 'creation of profit (43.1%)'. Conclusion : As farming career and medicinal crops farming career increased, environmental factors such as climate and pest problems affected in cultivation stage significantly, further uncertain market price gave a large factor in sales stage.

Background : Cnidium officinale M. is a medicinal plant used a lot of herbal and functional food ingredients. Recently, the area of cultivation has been increased due to the increase in domestic demand. By the way, this plant is very vulnerable to high temperature, and recently it is difficult to cultivate due to abnormal high temperature and so on. Therefore, in this study, in order to effectively reduce the high temperature of the field, we investigated the degree of temperature reduction and the growth condition of the plant after installed mulching, irrigation and the fog facilities. Methods and Results : In order to reduce the temperature of C. officinale M. cultivation field, the black and white double vinyl mulching, drip irrigation and fog spray treatment were applied and the results were as follows. The survey was conducted at about 2:00 pm on a clear day in August, and the temperature was about 33℃ at the time of measurement. In the case of only black vinyl mulching without watering, the soil temperature was 43.5℃, the surface temperature was 61.4℃, and the mortality rate of the C. officinale M. was 98.0%. The temperature of soil and surface were lowered to 33.1℃ and 38.6℃, respectively, when treated with black vinyl mulching and drip irrigation and fog spraying. In the case of black and white double vinyl mulching, the effect of temperature reduction was better. The soil surface temperature of the non-irrigation treatment was 37.9℃, the surface temperature was 48.5℃ and the mortality rate was decreased to 6.7%. In case of combined drip irrigation and fog spraying, soil temperature was reduced to 31.5℃, surface temperature was 35.8℃, and mortality rate was 0%. Conclusion : As a result of this study, the temperature of soil and surface dropped by up to 12. 0℃ and 25.6℃, respectively, when combined with black and white double vinyl mulching and drip irrigation and fog spray treatment. The mortality rate declined from 89.0% to 0%. Therefore, Therefore, if the results of this study are applied to the cultivation of C. officinale M., it is expected that the production stability will be improved.

Background : This study was conducted to investigate the seedling, growth, and yield characteristics of 1-year-old Polygala tenuifolia by cultivation of different regions. Methods and Results : After growing the seedlings for 2 months in the greenhouse, they were planted at different altitudes including: 100 m or less (Cheongju), 250 m (negative), 300 m (Jecheon), and 500 m (Pyeongchang) in the first week of May. The flowering period, fertilization period, fruit weight, growth, and yield were recorded and investigated in that period. Results of analyzing the physicochemical properties of the soils revealed that the pH of the soil was close to neutrality of 6.7 - 7.3 at 100 m, 250 m, and 500 m altitudes and an acidity of pH 5.7 at 300 m altitude. The average temperature for different time periods and altitudes are the following: 3.8℃ from June to October during the harvesting season, 21.8℃ at 100 m altitude in the end of June, 10.8℃ at 500 m altitude in the middle of harvest period, and 2.7℃ higher at 100 m altitude than 500 m altitude. The difference in the number of rooting (between 11 and 12/㎡) and the rate of rooting (82 - 92%) was not significant. The plant length was 25 - 26.5 ㎝ at 300 m and 500 m altitude. The largest number of branch was at 1.8 with 250 m altitude and largest stalk diameter at 500 m altitude at 6.0 ㎜. Leaf length and leaf width were the same per area, but the branches were the largest at 500 m at 17.9. Dry weight of 12.7 g was heaviest at 500 m altitude implying that the higher the altitude, the higher the dry weight will be. Moreover, in the 500 m altitude, root length of 29.2 ㎝ was the longest, root diameter was 5.9 ㎜, and the dry root weight of 7.9 g was the heaviest. However, the number of supporting root was 4.9 - 7.3 which was not statistically significant. The optimal yield per 10 a was 94 ㎏ at 500 m altitude. This was very high compared to yield of 28 ㎏ at 100 m altitude. The seed weight per 10 a was 1.5 ㎏ and 2.3 ㎏ at 100 m and 300 m altitude, respectively. The highest content of Teuifolin contained in the root was 124.07 ppm at 500 m altitude. Conclusion : Results showed that 1-year-old P. tenuifolia had optimal growth, higher yield and better vital component content at altitude of 500 m.

As an approach for estimation of the droplet size in the molten salt-liquid metal extraction process, a droplet formation experiment at room temperature was conducted to evaluate the applicability of the Scheele-Meister model with water-mercury system as a surrogate that is similar to the molten salt-liquid metal system. In the experiment, droplets were formed through the nozzle and the droplet size was measured using a digital camera and image analysis software. As nozzles, commercially available needles with inner diameters (ID) of 0.018 cm and 0.025 cm and self-fabricated nozzles with 3-holes (ID: 0.0135 cm), 4-holes (ID: 0.0135 cm), and 2-holes (ID: 0.0148 cm) were used. The mercury penetration lengths in the nozzles were 1.3 cm for the needles and 0.5 cm for the self-fabricated nozzles. The droplets formed from each nozzle maintained stable spherical shape up to 20 cm below the nozzle. The droplet size measurements were within a 10% error range when compared to the Scheele-Meister model estimates. The experimental results show th

Background : During 2016 to 2017, Bacterial Rot symptom has been observed on Atractylodes macrocephala Koidz. in Yeongju-si, Mungyeong-si, Jecheon-si and Eumseong-gun. This experiment was carried out to identify pathogenic bacteria that has not been reported up to now from A. macrocephala and to test pathogenicity of isolated bacteria against A. macrocephala. Methods and Results : Nine types of representative bacteria strains depending on colony size and color were isolated from surface disinfested symptomatic tissue that was macerated and streaked onto lysogeny broth (LB) medium with agar. Fungi were not recovered from any tissue that was surface disinfested and placed into acidified potato dextrose agar. Only one strain cause dark brown leaf rot symptom on A. macrocephala leaves soaked in bacterial suspensions. Potted A. macrocephala plants were used to test for pathogenicity. Inoculum was prepared by suspending the bacteria in sterile distilled water (SDW) for a final concentration of approximately 105 CFU/㎖. Suspensions were sprayed until runoff onto three replicate plants. Control plants were sprayed with SDW until runoff. Plants maintained in a dew chamber with 100% relative humidity at 30℃. After 3 days, leaf rot lesions developed on all inoculated plants; lesions later turned dark brown and appeared similar to symptoms observed in the field. Plants treated with water developed no symptoms. Same bacteria re-isolated onto LB from symptomatic tissues. Conclusion : On the basis of 16S rRNA sequence analysis, the strain isolated from A. macrocephala was identified as Pseudomonas viridiflava. Biological assay method using Potted plants confirmed the pathogenicity of Pseudomonas viridiflava. This is the first report of bacterial rot caused by Pseudomonas viridiflava on A. macrocephala.

Background : To evaluate the quality of Glycyrrhizae radix (licorice), a popular herbal medicine in Korea, it is necessary to investigate the component profiles of the Glycyrrhiza species distributed in Korean market. Specifically, to control the quality of Korean Glycyrrhizae radix preparations including raw materials, glycyrrhizin (> 2.5%) and liquiritigenin (> 0.7%) has been used as a standard marker compound in Korea Food and Drug Administration (KFDA). To the best of our knowledge, there have been only a few studies regarding the fingerprinting techniques and metabolomics approach of the phytochemicals in Glycyrrhizae radix in Korean market. Therefore, it is necessary to develop a validated method for botanical raw material (BRM) standardization. Methods and Results : Twenty-one Glycyrrhizae radix (G. glabra, G. uralensis, and G. glabra × G. uralensis) were analyzed by UPLC-QTOF-MS and were estimated to statistical and multivariate analysis. Large scale multi-targeted metabolic profiling and fingerprinting techniques were utilized to access a broader insight into the chemical composition of Glycyrrhiza species. The UV/VIS spectra, and MS data (accurate mass and fragmentation pattern in negative mode) of the components in the BRM of G. glabra, G. uralensis, and G. glabra × G. uralensis (incorporation and hybridization) were analyzed with multivariate analyses. Interestingly, UPLC-QTof-MS derived PCA analysis and fingerprinting techniques was found to be effective techniques in the classification of species based on genetic and/or geographical origins. In addition, the revealed metabolite markers could be utilized as a guidance for the BRM standardization as well as a database for quality control of commercial products containing Glycyrrhizae radix. Conclusion : From the above results, the revealed metabolite markers could be utilized as a guidance for the BRM standardization as well as a database for quality control of commercial products containing Glycyrrhizae radix.

Background : Licorice (Glycyrrhiza uralensis F.) is one of the most widely used raw materials for food and pharmaceuticals, and is currently the No. 1 imported medicinal plant in Korea. Efforts to settle licorice cultivation in Korea have been carried out for hundreds of years but have not succeeded, and many people suspect that the reason is due to the inadequate cultivation environment. However, even until recently, most licorice has been procured by wild harvesting, so studies on licorice cultivation technique and cultivation environment have not been conducted much in the world. This experiment was conducted to investigate the germination characteristics of licorice by temperature and salt concentration in order to find the optimal cultivation environment conditions of licorice. Methods and Results : The germination rate of licorice seeds was high 77.5 - 90.5% at 10 - 3 6℃, but it started to drop from 66.5 - 68.0% at 38 - 40℃ and it decreased sharply from 9.0 - 15.0% at 42 - 46℃. No longer germinated at temperatures above 48℃. The average number of days of germination was 12.3 days at 10℃, 5.7 days at 20℃, 3.5 days at 30℃ and 4.1 days at 40℃. The germination start date was 2 to 4 days in the 18℃ - 46℃ range. The average germination rate was the highest at 32℃. The germination time of 40% was 2 - 4 days at 18 - 46℃. The germination of more than 80% took 3 - 5 days at 20 - 40℃, 6 - 8 days at 14 - 1 8℃ and 10 days at 10 - 12℃. Licorice was able to germinate even in the presence of salt. Seed germination rate was 78.5 - 85.5% at 0 - 1% salt concentration and 63% at 1.5% and 18% at 2.0%. In addition, 8.5% seeds germinated successfully even at 3.0% salt concentration. Conclusion : Licorice has been known as a low temperature crop, but germination was possible in a wide range of temperature and salt concentration. Especially, the germination characteristic was higher in the high temperature range of 30 - 36℃, which corresponds to the domestic summer temperature. Therefore, the results of this study are expected to be useful reference for exploring suitable cultivation area in Korea.

Background : This experiment was conducted to improve the farm income by decreasing the rate of corrosion and increasing the emergence rate by selecting the proper storage method of the medicinal crop seed root. Methods and Result : Ten kinds of medicinal crop seed roots of Glycyrrhiza uralensis Fischer, Salvia miltiorrhiza Bunge, Codonopsis lanceolata, Platycodon grandiflorum, Atractylodes japonica, Angelica acutiloba, Adenophora triphylla, Rehmannia glutinosa, Angelica gigas and Cnidium officinale were used as test materials. In experiment 1, storage experiments were carried out using four treatments: untreated (dry in advance), untreated, filler (dry in advance) and filler. Drying in advance was carried for 2 days in dark place and the filler was vermiculite. For storage, in styrofoam box, seed roots were laid down in 3 replicates of 10 sets, stored in – 1℃ warehouse. The storage temperature was set as long as each seed root was not frozen investigating freezing point, supercooling point. As a result, the rate of corrosion was significantly lower in the 4th treatments (filler), such as A. japonica (30%) and A. gigas (10%), compared with untreated one. Therefore, in experiment 2, six treatments were carried out using filler. There were 6 treatments: filler disinfectant (dry in advance), filler disinfectant, filler biochar (dry in advance), filler biochar, filler disinfectant biochar (dry in advance) and filler disinfectant biochar. The disinfectant was used by metalaxyl-M at a concentration of 0.4 g/ℓ, and a biochar was used at a volume ratio of 50% (v/v). As a result, the rate of corrosion was significantly lower in the 6th treatment (filler disinfectant biochar), such as S. miltiorrhiza (13.3%) and C. lanceolata (6.7%). Conclusion : In storing medicinal crop root, it is better to store using fillers. It was shown that a mixture of filler and disinfectant biochar reduced the rate of corrosion.