논문 상세보기
pp.129-138
Eukaryotic translation initiation factor 5A (eIF-5A) is essential for proliferation of eukaryotic cells, andwas identified as diagnotic marker in cervical intraepithelial neoplasia, cervical and endometrial cancer, but relatively little is known about thein vivo and in vitro expression patterns of eIF-5A in oral premalignant and malignant lesions mirror the expression levels observed in vitro with cells derived from normal oral mucosa, immortalized oral keratinocytes (IHOK) and primary and metastatic oral squamous cell carcinoma (OSCC). We used an oral squamous cell carcinoma (OSCC) progression model composed of cell lines and tissue specimens to characterize expression patterns by Western blotting and immunohistochemistry. eIF-5A and PCNA levels are elevated in IHOKand primary and metastatic OSCC cella as compatred to normal human oral keraitinocytes. eIF5A and PCNA expression was l imited to basal cells of normal oral mucosa. eIF-5A and PCNA expression is increased in dysplastic epithelium spreading to more superficial layers, and its expression levels correlated significantly with the degree of dysplasia. Well and moderately differentiated OSCC showed strong expression of eIF-5A and PCNA. These results suggest that upregulated expression of eIF-5A seems to be an important epigenetic alteration that accompanies oral carcinogenic progression, and eIF-5A could be used as an biomarker for oral premalignat lesion or squamous cell carcinoma
II. MATERIALS AND METHODS
1. Cell culture
2. Western blot analysis
3. Tissue samples
4. Immunohistochemical Stain
5. Quantification of eIF5A-1 and PCNA expression
III. RESULTS
1. eIF5A-1 and PCNA protein expression innormal, immortlaized and oral squamous cellcarcinoma cell lines
2. eIF5A-1 and PCNA protein expression inexpression in benign, premalignant and malignantlesions of oral mucosa
IV. DISCUSSION
V. CONCLUSIONS
VI. REFERENCES
