Background : Chrysanthemum indicum L. is used in medicine, for bee, ornamental as multivoltinism plant resources. Since ancient times, Chrysanthemum indicum L. had been widely used as drug and tea. To diversity and increase the demand of Chrysanthemum, it is breeding for medicinal herb, tea and ornamental flower. Therefore size and shape of plant, color of flower have been used for cross breeding. CI2013 lines collected from Jeju have been used for landscape and ornament. and CI2010 lines collected from Sancheong have been used for medicinal herb tea. As a result, many new lines were developed. Therefore it was carried out to use as a date for new varieties through the characterization of good lines. Methods and Results : 7 lines were investigated and these plants were forstered and cultivated from Gyeongsangnam-do Agricultural Research & Extension Services (Hamyang). Breeding Lines were CI201001, CI201002, CI201005, CI201006, CI201303, CI201307, CI201401. The shape of CI201303 and CI201307 were round, plant height is low and width is wide, it may be used for landscape and ornament. As the size of flower in CI201005 was big, it may be used for medicinal herb tea. Conclusion : CI201303 and CI201307 lines were good for landscape and ornament because of round shape. CI201005 line was good for medicinal herb tea because of big flower.

Background : Cucuma longa L., in the family Zingiberaceae, is distributed in tropical and/or sub-tropical regions mainly in India and China. This species is commonly called tumeric, powder is used as medicinal herbs and/or flavor enhancer. It has been cultivated in southern region mainly Jindo. However, it might be possible to extend cultivation region due to rise in average temperature. In order to select superior lines, agronomic characteristics is commonly used. Because this is not the ultimate solution, the DNA marker approach has benefited the modern plant breeding. Therefore an easy approach by using one kind of primer have been developed from random amplification of polymorphic DNA sequences (RAPD) to discriminate effectively between different cultivars of Cucuma species Methods and Results : DNAs were extracted from the harvested roots of Cucuma sp. using DNeasy plant Mini kit (Qiagen, Hilen, Germany). These plants cultivated from GARES (Hamyang) and used for PCR amplification. The relative concentration of the extracted DNA was estimated Nano Drop ND-1000 (NanoDrop Technologies, Wklmington, De, USA) and final DNA concentration was adjusted to 5.5 ng/㎕. In this study 9 primer pairs were tested on 8 Cucuma sp. These primers showed polymorphism in Cucuma sp. The cluster dendogram showed that the similarity coefficients ranged from 0.68 to 0.87, CUR02 turned out to be CUR11, and CUR16 is similar to CUR17. Conclusion : These finding could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Cucuma species. These data on polymorphism difference based on RAPD will be give us invaluable breeding information by selection of superior lines.

Background : Chrysanthemum indicum L. is used in medicine, for bee, ornamental as multivoltinism plant resources. The purpose of this study was to evaluate methods of rooting promotion to make high quality medicinal plants, thereby increasing farm income. This study investigated the effects of different cuttage period, extraction site, shading degree, nursery tray for promoting cutting slips rooting in Chrysanthemum indicum L. Methods and Results : It is used New variety "Gamkuk 1" that were forstered in Gyeongsangnam-do Agricultural Research & Extension Services. Method of application with cuttage period were conducted under five condition; Around the middle of April, Toward the end of April, Early in May, Around the middle of May, Toward the end of May. Early in May plant length was longer than other cuttage period as 41.2%, 83.7% and are significant at significance level 0.05. Method of application with extraction site were conducted under three condition; 1 - 3 node, 4 - 6 node, 7 - 9 node. Leaf number in 1 - 3 node was more than other extration site as 19.4%, 33.6%. Also root length in 1 - 3 was more than other extration site as 10.5%, 23.2%. Method of application with shading degree were conducted under three condition; 50%, 70%, 90%. Plant length in 50 - 70% was longer than 90% shading degree as 23.8%, 24.2%. Also shading degree 50 - 70% had many root length and root number. Method of application with nursery tray were conducted under five condition; 72, 105, 128, 162, 200 tray. Plant length in 105, 128 tray was longer as 8.5 - 35.3% than other nursery tray and are significant at significance level 0.05. Conclusion : According to the results, Early in May, extraction site 1 - 3 node, shading degree 50 - 70%, nusery tray 105, 128 showed the highest growth on cutting slips root promotion in Chrysanthemum indicum L.