Extrachromosomal DNA (ecDNA) has appeared as a key driver of oncogene amplification, tumor heterogeneity, and therapeutic resistance, thereby reshaping the paradigm of precision oncology—particularly in metastatic and treatment-refractory cancers. However, the clinical translation of ecDNA-based discoveries into scalable therapeutic applications remains limited. This study introduces an integrated, empirically validated precision oncology platform that combines ecDNA biomarker profiling, artificial intelligence (AI)-driven multiomics modeling, and clustered regularly interspaced short palindromic repeats (CRISPR)-based functional validation. Built upon the “9 Building Blocks” business model, the platform incorporates high-resolution ecDNA detection (via whole-genome sequencing, fluorescence in situ hybridization, and AmpliconArchitect), single-cell and spatial transcriptomics, and machine learning-based therapeutic response prediction. The AI-integrated multi-omics framework achieved robust predictive accuracy (area under the curve > .90) across diverse tumor types and was functionally validated through patient-derived xenograft models and CRISPR-Cas9 gene screening. Specifically, CRISPR knockdown experiments that target ecDNA-associated oncogenes (e.g., Myelocytomatosis oncogene, epidermal growth factor receptor) demonstrated high knockdown efficiency (up to 82% using quantitative polymerase chain reaction), significantly reduced cell viability (up to 45%), increased apoptosis (up to 38%), and suppressed tumor growth in vivo (up to 53% reduction in volume, p < .001), confirming their biological and therapeutic relevance. A pilot study that involved 12 patients further demonstrated concordance between liquid biopsy-derived ecDNA profiles and tissue biopsy results, whereas a structured stakeholder survey (n = 47) yielded strong consensus on the platform’s clinical relevance (4.62/5), feasibility (4.45/5), and strategic value (4.58/5). These results underscore the platform’s technical robustness, translational potential, and commercialization readiness for ecDNA-guided precision therapeutics.

Nesfatin-1, an anorexic nucleobindin-2 (NUCB2)-derived hypothalamic peptide, controls appetite and energy metabolism. Recent studies show that nesfatin-1/NUCB2 is expressed not only in the brain but also in gastric and adipose tissues. Thus, we investigated the distributions of nesfatin-1/NUCB2 in various tissues of male and female mice by real-time PCR, western blotting, and immunohistochemical staining. Real-time PCR analyses showed that NUCB2 mRNA was predominantly expressed in the pituitary and at lower levels in the hypothalamus, spleen, thymus, heart, liver, and muscle of both male and female mice. Expression was much higher in reproductive organs, such as the testis, epididymis, ovary, and uterus, than in the hypothalamus. Western blot analysis of the nesfatin-1 protein level showed similar results to the real-time PCR analyses in both male and female mice. These results suggest that nesfatin-1/NUCB2 have widespread physiological effects in endocrine and non-endocrine organs. In addition, immunohistochemical staining revealed that nesfatin-1 was localized in interstitial cells, including Leydig cells and in the columnar epithelium of the epididymis. Nesfatin-1 was also expressed in theca cells and interstitial cells in the ovary and in epithelial cells of the endometrium and uterine glands in the uterus. These results suggest that nesfatin-1 is a novel potent regulator of steroidogenesis and gonadal function in male and female reproductive organs. Further studies are required to elucidate the functions of nesfatin-1 in various organs of male and female mice.

본 연구는 2011년 일본 후쿠시마 원자력 발전소 방사능 누출사고가 방사능 오염으로 인한 유아안전에 대한 유치원교사의 인식에 미치는 영향을 분석하고, 유아안전 대책마련을 위한 기초자료를 제공하는 데 의의가 있다. 본 연구는2013년 2월 C도의 공 사립유치원 교사 197명을 대상으로 설문지를 배부하여 회수된 134명의 자료를 분석하였다.본 연구의 결과, 일본 원전사고는 방사능 오염으로 인한 유아안전에 대한 교사의 불안감에 영향을 미쳤으나, 방사능오염으로 인한 유아안전에 대한 유치원 교사들의 안전지식과 대처방안은 낮은 것으로 나타났다. 결론적으로 방사능 오염으로부터 유아안전을 위한 하나의 방안으로 유아교사교육이 요구되며, 유아안전을 위한 대책 마련이 시급하다.

본 연구는 2011년 일본 후쿠시마 원자력 발전소 방사선 누출사고가 방사능 오염식품과 방사선 조사식품에 관한 유치원 부모의 인식에 미치는 영향을 분석하여 국민건강자료를 제공하는데 의의가 있다. 본 연구는 2012년 8월 J시의 K와 M유치원의 학부모 205명을 대상으로 설문지를 배부하여 회수된 178명의 자료를 분석하였다.본 연구의 결과, 일본 원전사고는 방사능 오염식품에 대한 학부모의 불안감에 영향을 미쳤으며, 방사선조사 식품에대한 부모들의 인식은 낮았다. 결론적으로 방사선조사 식품에 대한 유치원 부모들의 인식을 높이고, 방사능 오염식품과의 명확한 구분 및 올바른 인식을 돕는 적극적인 홍보와 부모교육이 이루어져야 할 것이다.