The sweetpotato whitefly, Bemisia tabaci, is a vector of more than 100 plantdiseased viruses as well as a serious pest to various horticultural crops. Virus acquisition affects the vector’s development and reproduction, but its mechanism is largely unknown. Here we compared the temperature responses between non-viruliferous and TYLCV-viruliferous Q biotype of B. tabaci. When both non-viruliferous and viruliferous whiteflies were exposed for 1 and 3 h at 4, 25, and 35°C, the mortality rate of viruliferous whiteflies is higher than nonviruliferous after exposure at 4°C and 35°C, but no differences at 25°C between them. Analysis of the expression levels of heat shock protein (hsp) genes using the quantitative realtime PCR showed that viruliferous whiteflies has higher expression in hsp70, and hsp90 at both 4°C and 35°C, but no differences at 25°C. The results suggest that vector insects may not be durable to unfavorable temperature conditions when they acquisite plant viruses.