Despite that porcine spermatogonial stem cells (pSSCs) have been regarded as a practical tool for preserving eternally genetic backgrounds derived from pigs with high performance in the economic traits or phenotypes of specific human diseases, there were no reports about precise definition of niche conditions promoting proliferation and maintenance of pSSCs. Accordingly, we tried to determine niche conditions supporting proliferation and maintenance of undifferentiated pSSCs for short-term. For these, undifferentiated pSSCs were progressively cultured in different composition of culture medium, seeding density of pSSCs, type of feeder cells and concentration of growth factors, and then total number of and alkaline phosphatase (AP) activity of pSSCs were investigated at post-6 day culture. As the results, the culture of 4x105 pSSCs on mitotically in activated 2x105 STO cells in the mouse embryonic stem cell culture medium (mESCCM) supplemented with 30 ng/ml glial cell line-derived neurotrophic factor (GDNF) was identified as the best niche condition supporting effectively the short-term maintenance of undifferentiated pSSCs. Moreover, the optimized short-term culture system will be a basis for developing long-term culture system of pSSCs in the following researches.

본 연구에서는 기존의 정량적인 강수량 정보를 제공하는 방식에서 벗어나 호우발생에 따른 생활환경의 변화에 끼치는 영향을 고려한 호우영향예 보서비스의 필요성을 기반으로 호우위험영향도 평가가 가능한 호우재해 위험영향 매트릭스를 개발하고, 이를 통해 호우위험영향을 평가하는 방법을 제시하였다. 사당동 일대를 대상으로 실제 발생 호우사상(2011년 7월 27일)을 적용하였으며, 호우에 의한 침수로 영향을 받는 대상별(사람, 교통, 시설) 호우위험영향평가를 수행하였다. 이를 위해 1 km 격자기반으로 호우위험정도(Impact Level)를 산정하고, 침수심 결과를 조합하여 격자 기반의 잠재호우위험영향(Potential Risk Impact)을 산정하였다. 여기에 강우발생가능성 Likelihood와의 조합을 통해 호우영향예보가 가능한 호우위험영향(Heavy Rainfall Risk Impact) 값을 산정하여 사당동 지역의 호우영향정도를 격자기반으로 4개의 등급으로 분석, 제시하였다.