간행물
한국동물생명공학회지 (구 한국수정란이식학회지) KCI 등재 Journal of Animal Reproduciton and Biotechnology
- 발행기관 한국동물생명공학회(구 한국수정란이식학회)
- 자료유형 학술지
- 간기 계간
- ISSN 2671-4639 (Print)2671-4663 (Online)
- 수록기간 1983 ~ 2024
- 주제분류 자연과학 > 생물공학 자연과학 분류의 다른 간행물
- 십진분류KDC 527DDC 636
권호리스트/논문검색
Vol. 29 No. 3 (2014년 9월) 14건
1.
2014.09
구독 인증기관 무료, 개인회원 유료
Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in 2.5x105cells/ml and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of IL-1β (0.1, 1, 10 and 100 ng/ml) were higher than without IL-1β, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p＜0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.
4,500원
2.
2014.09
구독 인증기관 무료, 개인회원 유료
Park, Min Hee, Park, Ji Eun, Kim, Min Seong, Lee, Kwon Young, Yun, Jung Im, Choi, Jung Hoon, Lee, Eunsong, Lee, Seung Tae
Despite that porcine spermatogonial stem cells (pSSCs) have been regarded as a practical tool for preserving eternally genetic backgrounds derived from pigs with high performance in the economic traits or phenotypes of specific human diseases, there were no reports about precise definition of niche conditions promoting proliferation and maintenance of pSSCs. Accordingly, we tried to determine niche conditions supporting proliferation and maintenance of undifferentiated pSSCs for short-term. For these, undifferentiated pSSCs were progressively cultured in different composition of culture medium, seeding density of pSSCs, type of feeder cells and concentration of growth factors, and then total number of and alkaline phosphatase (AP) activity of pSSCs were investigated at post-6 day culture. As the results, the culture of 4x105 pSSCs on mitotically in activated 2x105 STO cells in the mouse embryonic stem cell culture medium (mESCCM) supplemented with 30 ng/ml glial cell line-derived neurotrophic factor (GDNF) was identified as the best niche condition supporting effectively the short-term maintenance of undifferentiated pSSCs. Moreover, the optimized short-term culture system will be a basis for developing long-term culture system of pSSCs in the following researches.
4,000원
3.
2014.09
구독 인증기관 무료, 개인회원 유료
The aim of the present study was to compare two different serum-free media, modified synthetic oviduct fluid (mSOF) and modified potassium simplex optimization medium (mKSOM) containing 20% RD (RPMI1640 + DMEM, 1:1 v/v) (RD-mKSOM), for in vitro culture (IVC) of bovine embryos. After in vitro maturation and fertilization, the presumptive zygotes were cultured in two different serum-free conditions for 7 days and 9 days to evaluate blastocyst formation and hatching, respectively. Serum supplemented conventional CR2 medium was used as control. After 7 day of culture, there was no significant difference in cleavage and blastocyst formation rates among three groups (mSOF, 59.3 and 30.1%; RD-mKSOM, 65.0 and 41.5%; control, 51.6 and 38.0%, respectively). Hatching rate was significantly higher in control (69.0%) than other experimental groups (mSOF, 22.0%; RD-mKSOM, 39.5%) (P＜0.0001 and P＜0.001, respectively). Although both serum-free conditions showed lower hatching rates than serum-added control, in serum-free groups, RD-mKSOM showed significantly higher hatching rate than mSOF (P＜0.001). In addition, one-step using RD-mKSOM may facilitate IVC procedure than two-step culture system. In conclusion, the results indicate that one-step RD-mKSOM is more suitable defined culture system for IVC of bovine embryos than two-step mSOF.
4,000원
4.
2014.09
구독 인증기관 무료, 개인회원 유료
생쥐 2세포기, 4세포기, 8세포기를 각 발생 단계에서 채취하여 동결 보호제를 첨가한 서로 다른 배양액에서 배양하고, 배아의 동결 보존 및 융해 시 급속 처리와 저속 처리 단계로 비교하여 이들 조건이 배아의 생존과 발현에 미치는 영향을 조사하여 다음의 결과를 얻었다. 동결 보호제로 처리하여 배양액을 달리한 경우, 급속 단계에서는 모든 배양액에서 비슷한 발생율을 보였고, 저속단계의 4세포기와 8세포기는 D-PBS에서 높은 발생율을 보였다(P＜0.05, P＜0.01). 배아의 발생 시기에 따른 동결 보존 후, 발생율은 2, 4, 8세포기로 넘어갈수록 발생율의 증가를 보여 8세포기에서 발생율이 가장 높았다(P＜0.01). 동결 보호제의 처리단계에 따른 발생율은 2세포기의 급속 단계에서는 유사하였으나, 4세포기와 8세포기는 저속단계에서 높은 발생율을 보였으며(P＜0.05), 특히 8세포기에서 가장 높았다(P＜0.01).
4,000원
5.
2014.09
구독 인증기관 무료, 개인회원 유료
본 연구에서 생쥐 초기 배아를 이용하여 동결 보존된 배아의 생존율 또는 발달율에 영향을 미치는 요인들의 상관관계를 알아본 결과, 배아의 발달 단계에 따른 동결 - 해동 후 생존율에 있어서는 2세포기 배아가 4~8세포기 배아보다 유의하게 높았으나(p＜0.01), 배 발달율에 있어서는 4~8세포기 배아가 유의하게(p＜0.01), 높아 생존율과 배 발달율과는 상관관계가 없는 것으로 사료된다. 또한, 동결 보호제에 따른 배아의 생존율에 있어서는 2, 4~8세포기 배아 모두 DMSO에서 유의하게 높았으나(p＜0.01), 배 발달율에 있어서는 EG가 DMSO에 비해 더 유의하게 높은 성적을 나타내어(p＜0.01, p＜0.05) 동해로 인한 상해가 적은 것으로 생각되어 2, 4~8세포기 배아에서 EG가 더 효과적인 동결 보호제로 사료되며, 동결 프로그램으로는 완만 동결 - 급속 해동법이 더 우수한 프로그램으로 보인다.
4,000원
6.
2014.09
구독 인증기관 무료, 개인회원 유료
Programmed cell death or apoptosis is associated with changes in K+ concentration in many cell types. Recent studies have demonstrated that two-pore domain K+ (K2P) channels are involved in mouse embryonic development and apoptotic volume decrease of mammalian cells. In cerebellar granule neurons that normally undergo apoptosis during the early developmental stage, TASK-1 and TASK-3, members of K2P channels, were found to be critical for cell death. This study was performed to identify the role of K+ channels in the H2O2-induced or cryo-induced cell death of mouse and bovine embryos. Mouse and bovine two-cell stage embryos (2-cells) exposed to H2O2 for 4 h suffered from apoptosis. The 2-cells showed positive TUNEL staining. Treatment with high concentration of KCl (25mM) inhibited H2O2-induced apoptosis of 2-cells by 19%. Cryo-induced death in bovine blastocysts showed positive TUNEL staining only in the cells near the plasma membrane. Cryoprotectant supplemented with 25 mM KCl reduced apoptosis slightly compared to cryoprotectant supplemented with 5 mM KCl. However, the combination of antioxidants (β-mercaptoethanol) with 25 mM KCl significantly decreased the rate of H2O2-induced and cryo-induced apoptosis compared to treatments with only antioxidants or 25 mM KCl. These results show that blockage of K+ channel efflux for a short-time reduces H2O2- and cryo-induced apoptosis in mouse and bovine embryos. Our findings suggest that apoptosis in mouse and bovine embryos might be controlled by modulation of K+ channels which are highly expressed in a given cell type.
4,000원
7.
2014.09
구독 인증기관 무료, 개인회원 유료
Kim, Hyun, Bok, Nan-Hee, Kim, Sung-Woo, Do, Yoon-Jung, Seong, Hwan-Hoo, Kim, Jae-Hwan, Kim, Dong Hun, Kim, Min-Kyu, Ko, Yeoung-Gyu
Recently, several approaches have been used to measure the oxygen consumption rates of individual embryos, but relationship between oxygen consumption and pregnancy rates of Hanwoo following embryo transfer has not yet been reported. In this study, we investigated the correlation between oxygen consumption rate and pregnancy rates of Hanwoo embryo using a SECM. In addition to, the expression of apoptosis-related genes was determined using real-time PCR by extracting RNA according to the oxygen consumption of in vivo embryo. First, we found that the oxygen consumption significantly increased in blastocyst-stage embryos (blastocyst) compared to early blastocyst stage embryos, indicating that oxygen consumption reflects the embryo quality (Grade I). The oxygen consumption or GI blastocysts were significantly higher than those of GII blastocysts (10.2x1014/mol s-1 versus 6.4x1014/mol s-1, p＜0.05). Pregnant rate in recipient cow was 0, 60 and 80% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0 and over 12.0x1014/mol s-1, respectively. Apoptosis regulatory genes, Hsp-70.1 were significantly increased in over-10.0 group than below 10.0 group but in Caspase-3, Bax and P53 gene, there was no significant difference. In conclusion, These results suggest that measurement of oxygen consumption maybe help increase the pregnant rate of Hanwoo embryos.
4,000원
8.
2014.09
구독 인증기관 무료, 개인회원 유료
본 연구에서 한우를 공란우로 사용하여 OPU 방법으로 가장 더운 계절의 hot season과 선선한 cool season의 두 계절의 차이에 따른 생성된 난포의 수, 난자 회수율, 난자 등급율, 수정율 및 배반포 발달 능력을 분석하여, 두 계절이 공란우의 번식 능력에 미치는 영향에 관하여 조사하였다. 1. 계절의 영향이 OPU 공란우의 난포 생성 수에 미치는 결과는 난포 생성 개수는 1154개(18.32±2.26), 971개(15.41±3.34)로 hot season 그룹이 유의적으로 높은 것을 알 수 있었다(p＜0.05). 2. 계절에 따른 난자 수 및 난자 회수율은 hot season 그룹의 475개(7.54±3.14), 41.16%로 cool season 그룹 448개(7.11±3.42), 46.14%와 비교하여 유의적인 차이가 없었다(p＜0.05). 3. OPU를 통하여 회수된 두 계절별 난자 등급은 Grade A는 Hot season 그룹 110개(1.75±1.86), Cool season 그룹 63개(1.00±1.46)로 hot season 그룹이 cool season 그룹과 비교하여 유의적으로 높았다(p＜0.05). 하지만 다른 등급인 Grade B는 87개(1.38±1.60) vs. 97개(1.54±1.39), Grade C는 166개(2.63±2.43) vs. 170개(2.70±2.04), Grade D는 112개(1.78±2.65) vs. 118개(1.87±1.86)로 hot season과 cool season 간의 유의적인 차이를 보이지 않았다(p＜0.05). 4. 계절에 따른 체외 수정 후의 수정률은 hot season과 cool season 각각 242(66.67%)와 209(63.3%), 배반포 발달율 214(58.95%) vs. 188(56.97%)로 수정률과 배반포 발달율은 유의적인 차이가 없었다(p＜0.05). 본 연구의 결과로 계절에 따른 영향에 의해 공란우의 난포생성수와 A등급의 난자 출현율에서 유의적인 차이를 보였다. 하지만 나머지 등급의 난자 출현율, 수정률 및 배반포 발달율은 차이가 없는 것으로 보아, 계절의 차이로 인한 한우 공란우의 번식 능력에 미치는 영향은 미비하다고 판단된다.
4,000원
9.
2014.09
구독 인증기관 무료, 개인회원 유료
This study was carried out to establish the system of OPU derived embryo production, management of recipients as well as offspring production. OPU derived embryo production system was carried out of aspiration of immature oocytes 2 times per week, total 24 times for 3 months by an ultrasonographic guided follicular aspiration system and then produced in vitro-produced blastocysts by in vitro maturation, fertilization and culture system. This work was collected total 13,866 oocytes, average 8.2±4.5 oocytes per session and 8,170 G1 + G2 grade oocytes, average 4.8 oocytes per session by 1,692 times session of total 71 donors for 4 years from 2010 to 2013. The rate of cleavage and blastocyst developmental competence were obtained 11,825 (85.3%) and 5,032 (36.3%) that was 7.0±3.8 cleaved embryos and 3.0±2.5 blastocysts per session. OPU derived embryo transfer were taken place in 2, 4, 6 and 7 local governments at 2010, 2011, 2012 and 2013 for 4 years and pregnancy rate were obtained 41.2, 43.9, 46.5 and 49.7% in each years. It means that pregnancy rate was continuously improved according of every year for 4 years. Pregnancy rate was significantly different according to individual local government in which was 62.7% in B, but 24.2% in F at 2012. Paternity identification was carried out total 26 offspring in C local government of 2012 and then confirmed 100% agreement of its analysis. In conclusion, the results obtained the possibility of mass production of elite cow embryos as well as offspring by OPU derived embryo production system, of which could be decreased the required time of genetic improvement.
4,000원
10.
2014.09
구독 인증기관 무료, 개인회원 유료
Sexed semen is commonly used for the production of calves of the desired gender. Gender selection is important in animal production industries. For example, female cattle are required for the dairy industry while males are preferred in the beef cattle industry. The present study was to assess the in vivo embryo production efficiency using the semen separated according to sex during superovulation in Hanwoo. Seventy Hanwoo donor cows were flushed on day 7 of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered on 7 days after the third insemination by flushing the uterus with embryo collection medium. KPN semen straws used artificial insemination contained 20 million sperm (total number 60 million per donor). Sex-sorted semen straws contained 4 million sperm (total number 12 million per donor). The results obtained were as follows: No differences were observed in the efficiency of superovulation rates on KPN semen 87%, and sexed semen 100%, respectively. The mean numbers of total embryos are each 12.58±8.31 and 13.25±7.86. The mean numbers of transferable embryos, sexed semen were significantly lower than KPN semen (3.75±1.98 vs. 8.23±6.07, P＜0.05). The rates of unfertilized embryos from superovulation using sexed semen were significantly higher than KPN semen (50% vs. 15%, P＜0.05). The rate of degenerated 2-cell embryos from sexed and KPN semen was 60.87% and 11.11%, respectively (p＜0.05). In conclusion, these results indicate that superovulation using sexed semen was useful, but efficient embryo production was important to reducing the damage caused by the Flowcytometer-based sperm sorting procedure.
4,000원
11.
2014.09
구독 인증기관 무료, 개인회원 유료
제주마 경주 능력 개량을 위한 기초 자료 확보를 위하여 혈통 정보량과 오류량이 유전 모수 추정에 미치는 영향을 분석하였다. 혈통 자료는 램덤으로 혈통 정보의 삭제(0~25%) 또는 오류 유발(0~25%)과 각 수준별 20회 반복으로 모의자료를 형성하였다. 분석에 이용된 형질은 제주마 1,000m 경주의 주파 시간을 이용하였고, 상가적 유전 분산의 추정은 DF-REMLAI 알고리즘을 이용하였다. 개체의 양부모에 대한 정보 유실량과 오류량의 증가는 선형적인 회귀관계로 상가적 유전 분산 성분(유실 b = -0.079, 오류 b= -0.114)과 유전력 추정치(유실, b= -0.006; 오류, b = 0.008)의 감소를 초래했다(p＜0.01). 특히 개체의 양부모에 대한 정보유실보다는 정보 오류가 상가적 유전분산량과 유전력 추정치를 보다 크게 왜곡하는 것으로 나타났다. 부 또는 모에 대한 정보의 유실과 오류의 증가도 대부분 오차 분산 성분과 영구 환경 분산 성분의 증가를 유발시키고, 상가적 유전 분산성분의 감소를 가져왔다. 원자료에서 얻은 모수를 기준으로 했을 때 양부모에 대한 정보의 유실량과 오류량이 1% 증가함에 따라 유전력은 각각 0.92% 및 1.39%씩 낮아지는 관계를 보였다 (p＜0.01). 제주마의 1,000m 주파 속도에 대한 유전력은 0.60으로 추정되었지만, 초기 집단에 존재하는 혈통 정보의 누락 및 오류를 고려한다면 실제보다도 10% 정도는 낮게 추정되고 있다고 사료되었다. 이와 같은 결과는 상가적 유전 분산량의 감소는 물론, 선발에 따른 제주마의 경주 능력에 대한 유전적 개량량에도 영향을 줄 것으로 판단된다.
4,000원
12.
2014.09
구독 인증기관 무료, 개인회원 유료
The objective of this study was to determine the breed differences in the 3'-untranslated region (UTR) of MC1R mRNA, which may be used to distinguish Korean brindle cattle (Chikso) from other breeds. We investigated the relationship between the variation of 3'-UTR of the MC1R mRNA and coat color among different breeds and the Korean brindle cattle with different coat colors. MC1R mRNA expression levels were determined in accordance with the coat color and hair colors of the tail. Total cellular RNA was extracted from the hair follicles of the tails in Hanwoo, Korean brindle cattle, Holstein and HanwooxHolstein crossbred cattle. After cDNA synthesis, PCR was performed. Sequences of the 3'-UTR of MC1R mRNA were analyzed. The 3'-UTR of the MC1R mRNA from different breeds of cattle did not show any variations. There were no variations in the 3'-UTR of the MC1R mRNA in Korean brindle cattle with different coat colors. The levels of MC1R mRNA expression in hair follicles of the tail varied substantially among the Korean brindle cattle with different coat colors, except yellow coat color. Correlation between the MC1R mRNA expression in the hair follicles of the tail and coat color may be present in the Korean brindle cattle, but not between the variations of 3'-UTR of MC1R mRNA and coat color. Further studies to determine the regulation of MC1R mRNA expression from the hair follicles of different coat colors will be beneficial in clarifying the role of MC1R in the coat colors of the Korean brindle cattle.
4,000원
13.
2014.09
구독 인증기관 무료, 개인회원 유료
It is imperative to know the reproductive parameters of the existing swine breed for profitable farming in Bangladesh. This study was designed to determine the reproductive parameters of sows at hilly areas. A total of 245 pigs with 47 breedable sows were included. The data on age at puberty, oestrous cycle length, oestrus duration, gestation length, interval between furrowing and onset of oestrus, first service pregnancy rate, service per pregnancy and number of piglets born per sow in local and cross bred sows were determined. The present study revealed that age at puberty of local and cross breed sows was 232.5±8.4, 221.3±6.9 days, respectively. Oestrous duration was 41.1±3.1 hours. The interval between furrowing and onset of oestrus was shorter in cross bred sows. There was no variation in values of oestrus cycle length and gestation length. The first service pregnancy rate was higher in both the local and cross bred sow. Number of piglets per sow per farrowing was 6.1±2.2 and 60.9% local sows gave birth of 3 to 5 piglets per farrowing whereas 75.0% cross bred sows gave birth of 6 to 9 piglets. These results suggest that reproductive parameters of local sow need to be improved for better production, and cross bred sows should be reared for obtaining expected productivity.
4,000원
14.
2014.09
구독 인증기관 무료, 개인회원 유료
2013년 11월부터 2014년 7월까지 가축유전자원시험장 장내에서 사육 중인 한우 및 칡소 206두(한우 187두, 칡소 19두)를 대상으로 연령별, 종별 그리고 성별로 각각 분류하고, 이들에 대한 혈액학적 수치를 확인하였다. 혈액학적 검사 결과에서 1년 이하에서 5년 이상으로 연령이 증가됨에 따라 RBC(12.0~9.5x106/μl로)와 PLT치(717~432x103/μl로)에서 각각 유의성 있는 감소(P＜0.05)가 인정되었다. 동일 연령(2~3년)의 한우군과 칡소군 간에서의 비교에서는 RBC(10.1~10.4x106/μl)와 HCT(44.8~42.1%), MCV(49.4~44.9 fl) 및 MCHC (28.9~31.1 g/dl)에서 두 품종 간의 차이점이 인정되었으며, MCV와 MCHC에서도 통계적 유의성이 인정되었다(P＜0.05). 또한, 백혈구계 검사 결과에서도 연령이 증가함에 따라 WBC 수치(1년 이하의 13.8에서 5년 이상의 9.5x103/μl로)가 감소하는 경향이 인정되었으며, 백혈구 분포의 백분율에서는 전반적으로 림프구가 46.2%를, 분엽형 호중구가 35.6%로 나타났다. 한우군과 칡소군 간의 백혈구계 비교에서는 칡소군의 분엽형 호중구치가 2.7x103/μl로서 한우군에 비하여 유의성 있는 저하(P＜0.05)가 인정되었으며, 림프구치는 5.2x103/μl로 4.7x103/μl 한우군에 비하여 높게 나타났다. 임신우군과 비임신우군 간의 비교 분석에서는 Hg(9.9 g/dl)과 HCT(40.5%)이 비임신군에 비하여 통계적 유의성이 있는 저하(P＜0.05)가 인정되었으며, 총 백혈구수와 분엽형 호중구수, 림프구수 모두 비임신우군에 비하여 높게 나타났지만, 통계적 유의성은 인정되지 않았다.
4,000원
