간행물
한국동물생명공학회지 (구 한국수정란이식학회지) KCI 등재 Journal of Animal Reproduciton and Biotechnology
- 발행기관 한국동물생명공학회(구 한국수정란이식학회)
- 자료유형 학술지
- 간기 계간
- ISSN 2671-4639 (Print)2671-4663 (Online)
- 수록기간 1983 ~ 2024
- 주제분류 자연과학 > 생물공학 자연과학 분류의 다른 간행물
- 십진분류KDC 527DDC 636
권호리스트/논문검색
Vol. 8 No. 2 (1993년 10월) 7건
1.
1993.10
구독 인증기관 무료, 개인회원 유료
To investigate the effect of EDTA on the in vitro development of blastomeres isolated from 2, 4, and 8-cell embryos(termed 1 /2, 1 /4 and 1 /8 blastomeres, respectively) of ICR strain mice, those were cultured in vitro in 35 mm culture dishes containing NaHCO-BMOC-3 medium supplemented with 10, 50, 100, or 500 M of EDTA at 37 for 72hrs. under the atmosphere of 5% and 95% air. EDTA supplementation of 10, 50, or 100 M to medium significantly(P<0.01) increased blastocyst formation rate compared with controls in 1 /2(58.3, 63.7, and 61.3% vs 21.6%), 1 /4(54.7, 57.5 and 62.2% vs. 2L3%), and 1 /8 blastomeres(46.2, 48.7, and 57.7% vs. 19.1%). Whereas, it was significantly(P<0.01) decreased to 4.5, 2.3, and 2.0% for 1 /2, 1 /4 and 1 /8 blastomeres, respectively by the EDTA supplementation of 500 M Both the nuclear number(P<0.05) and diameter of blastocysts(P<0.01) developed from balstomeres were significantly affected by the origin of blastomeres. The nuclear number of blastocysrs developed from 1/2, 1/4, and 1/8 blastomeres ranged 28.3i1.3, 24.18i1.2, and 19.840.9, respectively. And the diameter of those blastocysts was 87.21.1, 56.40.9, and 39.20.8 M, respectively.
4,000원
2.
1993.10
구독 인증기관 무료, 개인회원 유료
To investigate the effect of extracellular matrix proteins on the in vitro development of ethanol-induced parthenogenetic eggs of ICR strain mice, those were cultured in vitro in fibronectin, gelatin, or collagen precoated culture dishes containing 1.5 ml of NaH-C03-BMOC-3 medium at 37 for 96 hrs. under the atmosphere of 5% and 95% air. Fibronectin, gelatin, or collagen significantly(P1.4, 45.4i1.4, and 44.8O.9, respectively. And the diameter of those eggs ranged 104.61.9, 102.82.3, and 103.4O.8 m, respectively.
4,000원
3.
1993.10
구독 인증기관 무료, 개인회원 유료
The studies on the carried out to investigate the effects of co-culture with uterine fluids and uterine epithelial cells on the in-vitro fertilization and developmental rate of porcine follicular oocytes. The ovaries were obtained from slaughtered swine. The follicular oocytes surrounded with cumulus cells were recovered by aspirating follicular fluids from the visible follicles of diameter 3~5 mm. The follicular oocytes were cultured in TCM-199 medium containing hormones and 10% ECS for 46~48 hrs in a incubator with 5% in air at 38.5 and then matured oocytes were again cultured for 12~18 hrs with motile capacitated sperm by preincubation of heparin. The results obtained in these experiments were summarized as follows ; 1.The in-vitro maturation and fertilization rate of porcine oocytes co-cultured with uterine fluids in TCM-199 medium were 68.0% arid 55.7%, the rates were higher than of control, 56.5% arid 38.7%. 2. When the in-vitro fertilized oocytes were co-cultured with porcine uterine epithelial cells, the fertilization rate was 60.3%, the rates were higher than that of control, 35.7%. 3. When the in-vitro fertilized oocytes were co-cultured with porcine uterine epithelial cells, the development rate to be blastocyst was 12.4%, the rates were higher than that of control, 9.2%(p<0.05).
4,000원
4.
1993.10
구독 인증기관 무료, 개인회원 유료
This study was conducted to determine the main effects of the parity and farrowing season on gestation length and newborn pigs on the basis of the data obtained from 234 litters of Landrace breeds raised at an integrated swine farm in Kyunggy province from January 1991 to December 1992. The results obtained are summarized as follows ; 1. The average gestation length was 115.37 days, and 114.64 days of 8th parity and over was shorter than those of other parities. 2. The averages of litter size and litter size alive per sow were 9.91 and 9.50 heads. The litter size horn at 1st parity was smaller than at other parities, and the litter size in spring was larger than in summer, autumn or winter. 3. The averages of birth weight per litter and pig were 13.53 kg and 1.37 kg. The effect of farrowing season for each litter weight(p<0.01) and pig weight(p<0.05) at birth was significantly higher in spring than other seasons. 4. Incidence of malformation and stillbirth at birth was 4. 10%, and it at 8th parity and over showed the highest rate(7.50%).
4,000원
5.
1993.10
구독 인증기관 무료, 개인회원 유료
This study was carried out to propagate Korean native cattle using beef recipients by embryo transfer. Seven Korean native cattle donors were superovulated with FSH 32mg and Embryos collected from donors were frozen and preserved in National Animal Breeding Institute. Frozen-thawed embryos were transferred to synchronized 40 beef recipients nonsurgically in Daekwanryeong Branch of National Animal Breeding Institute. The results obtained were as follows : 1. Total ova and transferable embryos per donor were 11.4 and 11.1 from 7 donors, respectively. 2. Among 40 recipients transferred with frozen-thawed embryos, 20 were pregnant(50.0%). 3. The pregnancy rate according to time from embryo thawing to transfer was higher when transferred within 3 hours than after 3 hours(57.6% vs. 14.3%). 4. The cow recipients showed slightly higher pregnancy rate than the heifer(53.3% vs. 48.0%). 5. Two grade embryos showed higher pregnancy rate than 1 grade(66.7% vs. 45.2%).
4,000원
6.
1993.10
구독 인증기관 무료, 개인회원 유료
The objective of this study was to produce calves derived from in vitro fertilization of in vitro matured follicular oocytes. Oocytes aspirated from small antral folicles of ovaries obtained at a local slaughter house were matured and fertilized in vitro. At l8hrs after insemination with Korean native cattle semen, oocytes were co-cultured for 6~7 days by utilizing co-culture system with bovine oviduct epithelial cell. After co-culture, good or excellent quality late morulae or early blastocysts were selected by morphological criteria under stereo microscope. Selected embryos were transferred to recipients on day 6 or 7 (estrus = day 0). Recipients were monitored by observation for estrus and rectal palpation after 60 days from embryo transfer. One of them went to term with the birth of a calf. This case is the first production of calf derived from in vitro fertilization in Korea.
4,000원
7.
1993.10
구독 인증기관 무료, 개인회원 유료
The present study was undertaken to determine the optimal condition for parthenogenetic activation of rabbit oocytes by electric stimulation in vitro in an attempt to develop nuclear transplantation techniques for cloning mammalian embryos and animals. Freshly ovulated oocytes were collected from superovulated rabbits from 13 to 26 hrs. after hCG injection. The cumulus-free oocytes were activated parthenogetically by repeated stimuli of square direct electric pulses in O.3M mannitol solution. After applying electric stimulations of different voltages, pulse durations and pulse times, all of the oocytes were cultured in TCM-199 with 10% FCS for 96 hours in a 5% incubator, and their developmental potential in vitro was examined. The higher activation rate (68.9%) was achieved at the voltage of 2.0kv/cm, the pulse duration of 60 sec and three pulse times and the activation rate of 100% was achieved at the pulse duration of 100 and 200 sec, the voltage of 1.5kv/cm and three pulse times. Although the higher rates of activation of oocytes were achieved at 100 and 200 sec, none of them developed to blastocyst in vitro. The oocytes collected 18~20 hours post hCG injection showed the highest rate of activation and development to blastocyst in vitro than the oocytes collected 13~15 or 25~26 hours post hCG injection. Therefore, it can be suggested that the application of electric stimulation of 2.0kv/cm, 60 sec and three pulse times to the oocytes collected at 18~20 hours post hCG injection would be more beneficial for the parthenogenetic activation of oocytes in rabbits.
4,000원
