간행물
한국동물생명공학회지 (구 한국수정란이식학회지) KCI 등재 Journal of Animal Reproduciton and Biotechnology
- 발행기관 한국동물생명공학회(구 한국수정란이식학회)
- 자료유형 학술지
- 간기 계간
- ISSN 2671-4639 (Print)2671-4663 (Online)
- 수록기간 1983 ~ 2024
- 주제분류 자연과학 > 생물공학 자연과학 분류의 다른 간행물
- 십진분류KDC 527DDC 636
권호리스트/논문검색
Vol. 17 No. 2 (2002년 8월) 10건
1.
2002.08
구독 인증기관 무료, 개인회원 유료
The experiment was divided into two phases. In phase-I fresh embryos were transferred and in Phase-II frozen embryos were transferred. Embryos were collected by using Dulbecco's phosphate buffered saline. In phase-I total of 65 ova were collected out of 107 ovulation in 18 goats. Recovery of ova was 60.74％, of which 51 (78.46％) was fertilized. Sixteen embryos were transferred to 10 recipient goats and kidding was observed in 6 goats, that produced 10 kids. Thus, 62.50％ embryo survival and 60％ kidding were achieved in phase-I. In phase-II of the experiment, 17 regular cyclic Black Bengal goats were used. The main purpose was to study the viability of caprine embryos after cryopreservation. In this phase the embryos were collected and frozen using Bio-cool freezers. A two step addition of cryoprotectants (5％ glycerol and 10％ glycerol) and three-step dilution of cryoprotectants with 1mole (M) sucrose was used. Embryos were preserved for 10 to 45 days. Out of 27 embryos preserved, 18 were recovered after freezing and thawing (37 water bath) with 33.33％ embryonic loss. Seventeen frozen and thawed embryos were transferred in 9 recipient goats, out of which kidding was observed in 6 goats and 7 kids were produced, giving a 66.66％ kidding and embryo survival of 41.17％. The technique utilized for fresh and frozen embryo transfer can be successfully utilized to produce goats of superior genetic merits. The protocol used for addition of cryoprotectant, freezing, thawing and dilution was found suitable for caprine embryo freezing.
4,000원
2.
2002.08
구독 인증기관 무료, 개인회원 유료
Human Prourokinase (proUK) offers potential as a novel agent with improved fibrin specificity and, as such, may offer advantages as an attractive alternative to urokinase that is associated with clinical benefits in patients with acute peripheral arterial occlusion. For production of transgenic cow as human proUK bioreacotor, we conducted this study to establish efficient production system for bovine transgenic embryos by somatic cell nuclear transfer (NT) using human prourokinase gene transfected donor cell. An expression plasmid for human prourokinase was constructed by inserting a bovine beta-casein promoter, a green fluorescent protein (GFP) marker gene, and human prourokinase target gene into a pcDNA3 plasmid. Cumulus cells were used as donor cell and transfected with the expression plasmid using the Fugene 6 as a carrier. To increase the efficiency for the production of transgenic NT, development rates were compared between non-transfected and transfected cell in experiment 1, and in experiment 2, development rates were compared according to level of GFP expression in donor cells. In experiment 1, development rates of non-transgenic NT embryos were significantly higher than transgenic NT embryos (43.3 vs. 28.4％). In experiment 2, there were no significant differences in fusion rates (85.4 vs. 78.9％) and cleavage rates (78.7 vs. 84.4％) between low and high expressed cells. However, development rates to blastocyst were higher in low expressed cells (17.0 vs. 33.3％), and GFP expression rates in blastocyst were higher in high expressed cells (75.0 vs. 43.3％), significantly.
4,000원
3.
2002.08
구독 인증기관 무료, 개인회원 유료
The present study was conducted for the production of transgenic cloned cows those secrete human lactoferricin into milk by somatic cell nuclear transfer (NT). To estimate detrimental effects of gene transfection on transgenic cloned embryo production, development rates of NT embryos were compared between transfected and non-transfected cumulus and ear fibroblast cells. An expression plasmid for human lactofericin (pbeta-LFC) was constructed by inserting a bovine beta-casein promoter, a green fluorescent protein (GFP) marker gene, and human lactoferricin target gene into a pcDNA3 plasmid. Two bovine somatic cell lines (cumulus cell and ear fibroblast) were established and transfected with the expression plasmid using a liposomal transfection reagent, Fugene6 as a carrier. Cumulus cell and ear fibroblast were transfected at the passage of 2 to 4, trypsinized and GFP-expressing cells were randomly selected and used for somatic cell NT. Developmental competences (rates of fusion, cleavage, and blastocyst formation) in bovine transgenic somatic cell NT embryos reconstructed with non-transfectecd cells were significantly higher than those from transfected cells in cumulus cell and ear fibroblast (P＜0.05). This study indicated that transfection of done. cell has detrimental effect on embryo development in bovine transgenic NT.
4,000원
4.
2002.08
구독 인증기관 무료, 개인회원 유료
본 연구는 소형견의 불임 해결을 위해 미숙난자를 보존 후 이용할 수 있는지의 여부를 판명하기 위하여 미성숙 난포란을 시간별로 배양한 뒤 vitrification 동결 융해후 체외수정율과 생존율을 조사하였다. 1 미숙난포란을 회수 후 1, 6, 12, 24시간 성숙 배양 후vitrification동결 융해 후 체외수정 시켰을때 수정율은 각각 31.4％. 22.5％, 11.9％ 및 5.3％로서 대조군의 수정율 60.0％에 비해 낮은 성적이었고, 회수 후 시
4,000원
5.
2002.08
구독 인증기관 무료, 개인회원 유료
The present study aimed at determining the effective dose of Folltropin, a follicle timulating hormone (FSH), on superovulation in indigenous cows of Bangladesh. Fifteen regularly cycling 5～7 years old dry cows, weighing 200～250 kg with 2.5～3.0 body condition scores (BCS) were divided into three groups (n＝5). Individual groups were superovulated with 100, 200 or 300 mg of Folltropin per animal. The superovulation treatment was initiated at Day 10 or Day 11 of the estrous cycle (Day 0＝day of estrus). Alfaprostol (6 mg) was injected to each cow 72 h after the initiation of superovulation treatment to induce eestrus. After confirming standing estrus, the cows were inseminated 2～3 times, 12 h apart, depending on the duration of estrus. At Day 6 or Day 7, individual horns of the uterus were flushed with 150～200 of phosphate buffered saline supplemented with BSA (0.2％), penicillin (100 IU／) and streptomycin (100 ／) using a two-way foley catheter. The embryos were concentrated, removing the excess medium through an embryo filter, and identified under a stereomicroscope. The identified embryos were collected, washed four times, evaluated and graded as excellent, good, fair or poor. The excellent, good and fair embryos were considered as transferable quality embryos. The mean (range). numbers of embryos collected vs. transferable quality embryos far 100, 200 and 300 mg of Folltropin were 4.5 (1～10) vs. 3.5 (1～8); 2.5 (1～4) vs. 1 (0～2) and 0.0 (0～0) vs. 0.0 (0～0), respectively, Folltropin at a dose of 100 or 200 mg produced suitable ovarian stimulation for superovulation in indigenous zebu cows of Bangladesh. A dose of 300 mg or more Folltropin consistently caused preovulatory corpora lutea formation in the ovaries and resulted in zero embryo recovery.
4,000원
6.
2002.08
구독 인증기관 무료, 개인회원 유료
본 연구는 체내, 체외 소 배반포기 배의 GMP vitrification 후 활력도의 비교와 한우 수정란을 몽골 소에 수정란이식 후 산자생산 가능성을 조사하고자 실시하였다. 한우 수정란은 체외수정란 또는 과배란처리에 의한 체내수정란을 생산하여 GMP vitrification 방법으로 동결 후 몽고로 수송하였다. 수란우는 CIDR과 처리에 의하여 동기화를 유도하였다 체내수정란생산을 위하여 7두를 과배란처리하였다. 총 64개의 배반포기를 회수하였다. (pe
4,000원
7.
2002.08
구독 인증기관 무료, 개인회원 유료
본 연구는 개의 동결정액 제조 시 동결보호 희석액 내에 첨가되는 당의 종류와 조합이 동결융해 후 정자의 생존율 및 운동성에 미치는 영향과 정자동결 시 straw size가 생존율에 미치는 영향에 대하여 조사하고자 실시하였다. 희석액은 Tris-citric acid extender (Tris-buffer)의 기본용액에 20% Egg-yolk, 8% glycerol, 1% Equex STM paste등을 첨가하였으며, 당 성분으로는 monosaccharid
4,000원
8.
2002.08
구독 인증기관 무료, 개인회원 유료
본 연구는 개 난자의 체외성숙율 높이기 위해 개의 발정주기가 체외성숙에 미치는영향과 체외성숙율의 효율적 향상을 위해 실험을 실시하였으며, 다음의 결과를 얻었다. 1. Anestrus, proestrus, estrus와 diestrus의 발정주기로 구분하여 MII로의 체외성숙율을 48시간과 72시간 배양후 관찰한 결과 각각 15.9%, 16.3%, 23.7%와 18.2%와 22.1%, 30.8%, 36.6%와 17.5%로 나타났다. 2. 각 발정주기별로
4,000원
9.
2002.08
구독 인증기관 무료, 개인회원 유료
본 연구에서는 한우의 신속한 증식을 위한 번식기술 개발을 위해 발정이 재귀되지않은 무발정 한우의 번식장애 치료효과와 공태기를 감축하기 위한 난소실질내 PGF／sub 2처치효과를 알아보았다. 1. 번식장애의 감별진단 결과는 검사두수 43두중 둔성발정 또는 발정발견의 잘못이 18두 (41.9%)로 가장 많았고, 난소기능정지 14두 (32.6%), 난포낭종 4두(9.3%), 영구황체 3두 (7.0%), 자궁내막염 2두 (4.7%), 자궁축농증과 황체낭종은 각
4,000원
10.
2002.08
구독 인증기관 무료, 개인회원 유료
1999년 6월부터 2002년 4월까지 총 2년 11개월 간 전국에서 발생한 소 유조사산에 대한 임상학적 연구결과는 다음과 같았다. 1. 계절별 소 유조사산 발생은 여름에 다른 계절에 비해 많이 발생하였다. 이는 여름철의 고온 다습한 기후와 낮은 우군관리 집중도에 기인한다고 사료된다. 2. 임신우의 산차별 유조사산 발생 비율을 조사한 결과 산차가 낮은 소에서 많이 발생하였다. 이는 유조사산의 원인이 임신우의 산차수에 있기보다는 산차수가 높은경우 사육되는
4,000원
