The objective of this study is to measure the changes of regional cerebral blood flow(rCBF) and blood pressure(BP) in rats, following the intravenous injection of drug. The measurement was continually monitored by laser-doppler flowmeter (Transonic Instrument, USA) and pressure tranducer(Grass, USA) in anesthetized adult Sprague-Dawley rats for about 2 to 2 and a half hours through the data acquisition system composed of MacLab and Macintosh computer. The result of this experiment was as following 1. Poria increased the changes of rCBF in rats, significantly. 2. The rCBF of Poria was significantly decreased by pretreated propranolol, but not by pretreated L-NNA. 3. Radix Cynanchi Wilfordii decreased the changes of rCBF in rats, significantly. 4. The rCBF of Radix Cynanchi Wilfordii was not changed by pretreated propranolol and L-NNA. 5. Poria and Radix Cynanchi Wilfordii increased the changes of rCBF in rats, significantly. 6. The rCBF of Poria and Radix Cynanchi Wilfordii was significantly decreased by pretreated propranolol, but not by pretreated L-NNA. 7. Poria decreased the changes of BP in rats, significantly. 8. The BP of Poria was significantly increased by pretreated propranolol and L-NNA. 9. Radix Cynanchi Wilfordii did not change the BP in rats. 10. The BP of Poria and Radix Cynanchi Wilfordii did not change by pretreated propranolol but was increased by pretreated L-NNA. According to the result above, Poria and Radix Cynanchi Wilfordii have a different effect on each other, extract together. It is considered that the effect of Poria and Radix Cynanchi Wilfordii increased that of each drugs, the increasing rCBF of Poria and Radix Cynanchi Wilfordii is mediated by adrenergic β-receptor.

본 연구는 개의 동결정액 제조 시 동결보호 희석액 내에 첨가되는 당의 종류와 조합이 동결융해 후 정자의 생존율 및 운동성에 미치는 영향과 정자동결 시 straw size가 생존율에 미치는 영향에 대하여 조사하고자 실시하였다. 희석액은 Tris-citric acid extender (Tris-buffer)의 기본용액에 20% Egg-yolk, 8% glycerol, 1% Equex STM paste등을 첨가하였으며, 당 성분으로는 monosaccharid

This study was conducted to evaluate whether \"Royal jelly\" (RJ) added to Tris-buffer dilute contributed to supporting post-thaw viability and longevity of frozen canine spermatozoa. Two Japanese spitzs (2 to 4 years of age) were used as a semen donor. Semen was collected by manual masturbation and separated into 3 fractions. Only the sperm-rich fraction having sperm motility of more than 70%, containing sperm concentration of 2~410 cells/ml and having dead or abnormal spermatozoa of less than 15% was used for the experiment. Each ejaculated semen was centrifuged at 400 g for 5 min and then diluted in a Tris-buffer supplemented with 20 ml egg yolk (Ext I), 4% glycero1 and 1% Equex STM Paste (Ext II) or g1ycero1, Equex STM paste and RJ of various concentrations (Ext II-RJ). After freezing and thawing, viability of spermatozoa in Ext II -RJ containing 1% RJ immediately after thawing (67.59.6) was significantly lower than that of Ext II , Ext II -RJ containing 0.01 or 0.1% RJ (77.512.5, 78.78.2 and 80.06.3). However, Ext II-RJ containing 0.1% RJ yielded higher viability than Ext II, Ext II-RJ containing 0.01% at or 1% 1 h after thawing (69.58.1 vs. 55.012.9, 57.59.6 and 41.512.6; P<0.05). At 1 h after thawing, the viability of spermatozoa thawed in 7 (68.812.5) was significantly higher than that of spermatozoa thawed in 38 (48.816.3), although there was no difference in the viability between both groups immediately after thawing (77.59.6 and 81.38.1). Post-thaw viability and longevity of post-thaw spermatozoa in Ext II-RJ containing 0.1% RJ was higher in those in Ext II at 1 h (65.012.9 vs. 42.512.6), 2 h (52.512.6 vs. 27.517.1) and 3 h (40.014.1 vs. 20.012.1) after thawing. These results indicated that addition of 0.1% af to Tris-buffer enhanced post-thaw viability and longevity of canine spermatozoa and this additive can be used for increasing the possibility of collision between spermatozoa and ova during insemination.emination.