This study was performed to analyze the causative agents of disease occurred in Korean black goat. Bovine viral diarrhea (BVD) and infectious bovine rhinotracheitis (IBR) known as main causative agents of diarrhea and abortion in cattle were founded in Korean black goats. Escherichia coli and Clostridium perfringens are the main causes underlying diarrhea in Korean black goats as well as other animals. In addition, the orf virus was found as a causative agent of contagious ecthyma that shows frequent occurrence in Korean black goats. Leptospirosis was found in Korean black goats with low occurrence rate. However, unlike the reports from many other countries, we could not detect the antibodies for Johne’s disease and Neospora caninum in Korean black goats. These results are likely to contribute to improving the productivity of raising black goats as a field of livestock farming.

The objective of this study was to investigate the relationship between body weight, body condition score (BCS), blood urea nitrogen (BUN), glucose, cholesterol and number of transferable embryos for the purpose of improving reproductive performance in Hanwoo donors. Seventy five cows, at random stages of the estrous cycle, received a CIDR together with injection of 1mg estradiol benzoate and 50 mg progesterone, and gonadotropin treatment begann. Four days later, the animals were superovulated with a total of 28AU FSH (Antorin, 2AU = 1 ml) administered twice daily in constant doses over 4 days. On the 3rd administration of FSH, CIDR was withdrawn and 25 mg was administered. Cows were artificially inseminated twice after estrous detection at 12 hr intervals. The cows received GnRH at the time of 1st insemination. Embryos were recovered 7 days after the 1st insemination. In conclusion, cows with body weight < 400, 400~450 and > 450kg had number of transferable embryos of , and , cows with BCS <2.25, 2.25~2.75 and had number of transferable embryos of , and respectively. These data indicate that a body weight and BCS for superovulation of CIDR-treated Korean native cows does not affect the embryo yield.

최근 사료 값의 상승과 한우 값의 하락으로 한우 사육농가의 어려움이 증대되고 있으 며, 한우 번식농가에서는 공태기간과 수태당 수정횟수의 증가 등에 의한 사육비용 증가 가 문제점으로 인식되고 있다. 따라서 본 실험에서는 한우 번식농가에서 분만후 공태기 간이 90일 이상인 한우와 3회 이상 인공수정하여도 임신되지 않은 저수태우의 생식기 상 태를 점검한 후 조기임신을 위해 배란동기화법인 Ovysynch와 CIDR-based TAI 처리에 의한 수태율을 조사하였다. 장기공태 한우 52두의 수정기록 및 분만기록을 확인 후 생식 기의 상태를 검사하였다. 자궁 및 난소 등을 검사한 결과 자궁 상태는 모두 정상이었으 나 1두는 허약(BCS 2.0)과 난소기능 휴지로 시험축에서 제외하였다. 장기공태우 51두 중 수정횟수 3회 이상인 저수태우는 13두로 평균 수정횟수 3.6회, 평균 공태일 123.8일, 평 균 산차 4.2회였으며, 분만후 공태기간이 90일 이상인 장기공태우 38두의 평균 수정횟수 는 1.8회, 평균 공태일 115.9일, 평균 산차 3.3회였다. 수정횟수 3회 이상인 저수태우 6두 에 Ovysynch를 처리하였고, 나머지 7두에 CIDR-based TAI를 처리하였다. 분만후 공태기 간이 90일 이상인 한우 38두는 19두씩 두 그룹으로 나누어 Ovysynch와 CIDR-based TAI를 처리하였다. Ovysynch 처리는 발정주기 임의의 시기인 Day 0에 gonadorelin (Gonadon, Dongbang, Korea) 100 μg를 근육주사하였고, Day 7에 dinoprost(LutalyseTM, Pharmacia, Belgium) 25 mg을 근육주사하였으며, Day 9에 gonadorelin 100 μg를 근육주 사하고 16시간 후 즉 Day 10에 한우 동결정액 1straw를 융해하여 인공수정하였다. CIDR- based TAI 처리는 Day 0에 gonadorelin 100 μg를 근육주사와 CIDRⓇ(InterAg, New Zealand)를 질내에 삽입하였고, Day 7에 CIDR를 제거하고 dinoprost 25 mg을 근육주사 하였으며, Day 9에 gonadorelin 100 μg를 근육주사하고 16시간 후 즉 Day 10에 한우 동 결정액 1 straw를 융해하여 인공수정하였다. 수정 횟수 3회 이상인 저수태우에서 수태율 은 Ovysynch 처리가 50%, CIDR-based TAI 처리가 57.1%였다. 분만후 공태기간이 90일 이상인 한우에서 수태율은 Ovysynch 처리가 63.1%, CIDR-based TAI 처리가 68.4%였다. 본 실험에서 장기공태우의 생식기 검사결과 52두 중 51두에서 정상적인 자궁과 난소상태 를 나타낸 것으로 보아 장기공태 한우의 대부분이 미약발정, 발정관찰 미흡 및 배란지연 등의 가능성을 추정할 수 있었다. 장기공태 한우 51두에 두 종류의 배란동기화법을 처리 한 결과 32두 임신으로 62.7%의 수태율을 나타내어 배란동기화법을 장기공태우에 적용 할 경우 양호한 수태율을 얻을 수 있을 것으로 생각된다. 하지만 본 실험에서는 장기공 태우에 Ovysynch와 CIDR-based TAI 처리에 의한 수태율에서 유의적인 차이를 나타내지 않았으므로 처리 비용측면에 있어서 Ovysynch 처리에 의한 배란동기화법이 효율적인 것 으로 추정된다.

The objective of this study was to investigate the relationship between estrous expression, body condition score (BCS), blood urea nitrogen (BUN) and number of transferable embryos for the purpose of improving reproductive performance in blood of Hanwoo donors. Sixty, at random stages of the estrous cycle, received a CIDR. Four days later, the animals were superovulated with a total of 28AU FSH (Antorin, 2AU=1 ml) administered twice daily in constant doses over 4 days. On the 3th administration of FSH, CIDR was withdrawn and 25 mg PGF2α was administered. Cows were artificially inseminated twice after estrous detection at 12 hr intervals. The cows received 100 μg GnRH at the time of 1nd insemination. Embryos were recovered 7 or 8 days after the 1st insemination. The estrous inducement rate and estrous expression rate were significantly lower for cows with BCS below 2.25 than for cows with BCS above 2.25. There was 50.0% of rate of mounting in cows with BCS below 2.25 whereas the rate of mounting was markedly increased in cows with BCS above 2.25 (94.1% and 89.5% for BCS 2.25～2.75 and BCS above 2.75 cows, respectively). Cows with BCS ＜2.25, 2.25～2.75 and ≥2.75 had number of transferable embryos of 4.5±0.7, 5.9±1.8 and 5.6±2.3 respectively.

The objective of this study was to investigate the relationship between concentration of urea nitrogen, glucose, cholesterol and number of transferable embryos for the purpose of improving reproductive performance in blood of Hanwoo donors. Fifty five, at random stages of the estrous cycle, received a CIDR. Four days later, the animals were superovulated with a total of 28AU FSH (Antorin, 2AU=1 ml) administered twice daily in constant doses over 4 days. On the 3th administration of FSH, CIDR was withdrawn and 25 mg PGF2α was administered. Cows were artificially inseminated twice after estrous detection at 12 hr intervals. The cows received 100μg GnRH at the time of 1nd insemination. Embryos were recovered 7 or 8 days after the 1st insemination. Cows with BUN ＜10, 11～18 and ≥19 mg/dl had number of transferable embryos of 4.3±1.3, 5.8±1.8 and 4.7±2.1 respectively. The mean numbers of total ova from < 10 and 10≤ of corpora lutea(CL) was 8.9 and 14.3, respectively. The number of transferable embryos differed between < 10 and 10≤ CL was 4.8 and 5.6, respectively.

The objective of this study was investigate the superovulation treatment and to relate concentrations of blood urea nitrogen(BUN) in Hanwoo donors. Thirty six, at random stages of the estrous cycle, received a CIDR. Four days later, the animals were superovulated with a total of 28AU FSH (Antorin, 2AU=1 ml) administered twice daily in constant doses over 4 days. On the 3th administration of FSH, CIDR was withdrawn and 25 mg PGF2α was administered. Cows were artificially inseminated twice after estrous detection at 12 hr intervals. The cows received 100 μg GnRH at the time of 1st insemination. Embryos were recovered 7 or 8 days after the 1st insemination. Cows with BUN ＜10, 11～18 and ≥19 mg/dl had return of estrus of 34.6, 30.5 and 30.4 days respectively. Return of estrus after superovulation treatment was not significantly lower for cows with blood urea nitrogen (BUN) above 10 mg/dl than for cows with BUN below 10 mg/dl. Cows with BUN ＜10, 11～18 and ≥19 mg/dl had number of transferable embryos of 3.2±1.2, 5.4±1.9 and 4.1±2.1 respectively.