This study was carried out to obtain new anti-gout agent from mushrooms. Various extracts from twelve kinds of mushrooms were prepared by water and ethanol extractions and its anti-gout xanthine oxidase inhibitory activity were investigated. Water extract of Flammulina velutipes and Agaricus bisporus fruiting bodies showed high xanthine oxidase inhibitory activity of 70.8% and 60.0%, respectively. However, its inhibitory activity of these ethanol extracts were very low excepet 60.3% of Innotus obliquus. Finally, we selected Flammulina velutipes, showing the highest xanthine oxidase inhibitory activity as a producer of new anti-gout agent.