Physical protection education was legislated by the Ministry of Education, Science and Technology (MEST) in November 2010. KINAC (Korea Institute of Nuclear Nonproliferation and Control) was designated as the exclusive institution for physical protection education and training by MEST in October 2011, and it has since functioned as the sole institution responsible for this critical aspect of nuclear security education in the country. Over the past decade, KINAC has undertaken a variety of training initiatives aimed at enhancing the capabilities of nuclear operators’ physical protection personnel. Furthermore, it has consistently pursued annual curriculum revisions based on insights gleaned from surveys and workshops. In conventional curriculum assessments, general surveys often rely on Likert scale or short-answer questions as primary indicators, mainly due to their ease of data processing. Descriptive questions, while capable of capturing diverse opinions, have historically been relegated to a secondary role owing to the inherent challenges associated with data analysis. While physical protection education has made concerted efforts to solicit diverse opinions through descriptive questions, difficulties in organizing and leveraging this valuable data have resulted in it primarily serving as reference material. This study introduces a novel approach by employing ChatGPT, a chatbot, to conduct a comprehensive analysis of the descriptive questions from the physical protection education survey administered in the first half of 2023. The primary objective is to formulate a robust plan for curriculum enhancement based on a wide spectrum of opinions. Following the completion of physical protection training by 2,014 individuals in the first half of 2023, a survey was distributed, yielding an impressive response rate of 95.7% with 1,927 respondents. Chatbots were harnessed to extract major keywords and perform frequency analyses on approximately 360 responses to descriptive questions in the survey. The analysis revealed that certain keywords emerged with notable frequency, in the following order: “drone” (mentioned 51 times), “access management” (mentioned 28 times), “inspection and search” (mentioned 27 times), and “cybersecurity” (mentioned 20 times). Further analysis of these major keywords and related content revealed a consensus among trainees that there is a pressing need to incorporate topics addressing drone threats and responses, as well as strategies to fortify access management into the curriculum. This study underscores the potential to harness standardized data analysis techniques to synthesize and integrate trainees’ subjective opinions, thereby providing a solid foundation for the refinement of the curriculum.

Direct injection of CRISPR/Cas9 into zygotes enables the production of genetically modified nonhuman primates (NHPs) essential for modeling specific human diseases, such as Usher syndrome, and for developing novel therapeutic strategies. Usher syndrome is a rare genetic disease that causes loss of hearing, retinal degeneration, and problems with balance, and is attributed to a mutation in MYO7A, a gene that encodes an uncommon myosin motor protein expressed in the inner ear and retinal photoreceptors. To produce an Usher syndrome type 1B (USH1B) rhesus macaque model, we disrupted the MYO7A gene in developing zygotes. Identification of appropriately edited MYO7A embryos for knockout embryo transfer requires sequence analysis of material recovered from a trophectoderm (TE) cell biopsy. However, the TE biopsy procedure is labor intensive and could adversely impact embryo development. Recent studies have reported using cell-free DNA (cfDNA) from embryo culture media to detect aneuploid embryos in human in vitro fertilization (IVF) clinics. The cfDNA is released from the embryo during cell division or cell death, suggesting that cfDNA may be a viable resource for sequence analysis. Moreover, cfDNA collection is not invasive to the embryo and does not require special tools or expertise. We hypothesized that selection of appropriate edited embryos could be performed by analyzing cfDNA for MYO7A editing in embryo culture medium, and that this method would be advantageous for the subsequent generation of genetically modified NHPs. The purpose of this experiment is to determine whether cfDNA can be used to identify the target gene mutation of CRISPR/Cas9 injected embryos. In this study, we were able to obtain and utilize cfDNA to confirm the mutagenesis of MYO7A, but the method will require further optimization to obtain better accuracy before it can replace the TE biopsy approach.

There are many different types of heating methods in the world. Most of these heating methods are through radiation and convection. These heating types emphasize insulation because it is important to maintain the indoor air temperature. In contrast, the ondol, emphasizes thermal storage in the floor because it heats conduction. In other words it heats through direct contact. This unique heating method has created a unique interior environment. Activities have to occur close to the floor so that people may come in direct contact with the heat, thereby influencing the cultural development of the Korean people. The ondol has also created an interior environment where it is wanner near the floor than the ceiling in contrast to other heating systems where the area close to the ceiling is warmer. This environment is inline with the optimal healthy conditions the Koreans prefer, the warm feet cool head environment.

There are many different types of heating methods in the world. Most of these heating methods are through radiation and convection. These heating types emphasize insulation because it is important to maintain the indoor air temperature. In contrast, the ondol, emphasizes thermal storage in the floor because it heats conduction. In other words it heats through direct contact. This unique heating method has created a unique interior environment. Activities have to occur close to the floor so that people may come in direct contact with the heat, thereby influencing the cultural development of the Korean people. The ondol has also created an interior environment where it is warmer near the floor than the ceiling in contrast to other heating systems where the area close to the ceiling is warmer. This environment is inline with the optimal healthy conditions the Koreans prefer, the warm feet cool head environment.