Herbicide resistance is the most common trait being tested and thus herbicide‐resistant genetically modified plants are now the most widely cultivated worldwide. Here we developed herbicide‐resistant transgenic Agrostis mongolica Roshev. by employing an efficient Agrobacterium‐mediated transformation procedure with 25.2% of transformation efficiency. The identification and employment of regenerable and reproducible type of callus was one of the most critical factors to ensure success in this study. PCR analysis confirmed that the bar transgene was integrated into the genome of transgenic plants. The expression of 35S‐bar gene was confirmed by Northern blot analysis. The transgenic plants showed complete resistance to herbicide, indicating that the bar gene is functional in transgenic plants.