Spermatogonial stem cells (SSC) undergo self-renewal division and generate spermatogenesis to produce mature spermatozoa. Very recently in some species, include rodent and farm animals, SSC has been isolated and cultured in vitro. In this study, we analysed SSC marker of both 5 days old and pubertal pig testis by histological method. In 5day pig testis, prior to set of spermatogenic differentiation, the seminiferous tubules contain a relatively large number of SSCs than in pubertal pig testis. Then putative pig SSCs were successfully isolated from 5 day pig testis, and cultured long term using CD34 positive cell culture media contained GDNF, bFGF, LIF and EGF. The SSC colonies were appeared at 3 days after cells were seed. The SSC colonies were alkaline phosphatase positive and strongly expressed PGP 9.5, PLZF and DBA, but not expressed GATA4 and LH receptors. The SSC colonies were stably proliferated in GDNF, bFGF, LIF and EGF contained CD34 positive cell culture media up to 60 days. This study will be facilitated to study of in vitro and ex vivo spermatogenesis and of production of transgenic pigs using sperm vector.

We investigated the structural parameters of a sample of 30 dwarf galaxies(15 dEs and 15 dS0s) in the Virgo Cluster using i-band images from the Sloan Digital Sky Survey Data Release 4. Among 28 galaxies for which surface brightness profiles were derived from ellipse fittings, 23 galaxies had a single component that was adequately described by a generalized Sersic function with a shape parameter ranging from n=0.5 to 2, while 5 galaxies(2 dEs and 3 dS0s) had bulge and disk components that were fitted by a generalized Sersic function and an exponential function, respectively. Since the majority of dwarf galaxies in the present sample had a single component, it seems likely that genuine dS0 galaxies that have disk and bulge components are quite rare in the Virgo Cluster. The similarity in structural parameters of genuine dS0 galaxies in the Virgo Cluster with those of Magellanic-type galaxies implies that the progenitors of dwarf lenticular galaxies in the Virgo Cluster were most likely Magellanic-type galaxies if dS0s are harassed late-type spirals.

구명 조끼는 익수자의 부력을 유지시키는 기능을 가지며, 낮은 해수 온도에서 저체온증에 빠지는 시간을 단축 시킬 수 있는 역 할을 할 수 있다. 본 논문은 서멀 마네킹 실험과 수치적 방법을 적용하여 단열성능을 향상 시키기 위해서 개발된 팽창형 구명 조끼와 폼형 구명 조끼의 단열성능 및 저체온증 방지 효과를 평가하였다. 단열성능 평가를 위해서 서멀마네킨을 이용하여 열유속 및 열저항을 계측하였으 며, 본 연구에서 제시된 구명 조끼의 단열성능을 기존의 제품과 비교하여 검토하였다. 또한 저체온증에 빠지는 정도를 상대적으로 파악하기 위해서 유한요소해석을 이용하여 구명 조끼의 종류에 따른 체온 저감 시간을 예측하고 이를 비교 평가하였다. 저체온증 예측모델은 Pennes의 신체 열전달 해석 모델을 기반으로 작성되었으며, 실험으로부터 계측한 열저항 값을 이용하여 대류 열전달 조건을 환산하여 계산되었다. 그 결과 본 연구에서 단열성능을 향상시키기 위해서 제시된 하는 구명 조끼가 기존 제품에 비해 단열성능이 우수하게 평가됨을 확인하였다.