The effects of a newly developed flower thinning formulation (FTF) on the vitality of the honey bee Apis mellifera were examined by measuring the activities of various digestive enzymes in adult worker bees. First, direct spraying of the FTF solution did not cause any behavioral changes or lethal effects for the honey bees based on 24 h observation. Second, oral ingestion of a sugar solution containing the FTF did not produce any significant change in the activities of amylase, proteinases, lipase, or acetylcholine esterase (AChE) in the worker bees 6 h or 24 h after treatment. Meanwhile, a commercial formulation containing sulfur compounds showed slightly reduced activities for several digestive enzymes and AChE, although no behavioral disturbance. Thus, the results of the present study suggest that the FTF is not toxic for honey bees, in terms of contact and ingestion. Therefore, this newly developed FTF can be used for flower thinning without any detrimental effects on pollinating insects.

Sweetpotato whitefly, Bemisia tabaci, is a vector of more than 100 plant-diseased viruses, as well as a serious pest of various horticultural plants. This species harbors a primary endosymbiont Portiera along with several secondary endosymbionts such as Cardinium and Hamiltonella. We investigated whether or not TYLCV acquisition alters the densities of endosymbionts in the body of B. tabaci using quantitative real-time PCR. Our results showed that the densities of both Cardinium and Hamiltonella, but not Portiera, increased upon acquisition of TYLCV. In addition, expression of GroEL, a molecular chaperone produced by Hamiltonella, was significantly upregulated in TYLCV-infected whiteflies. Our results suggest that endosymbionts may play an important role in TYLCV transmission mechanism within the body of B. tabaci.

Whiteflies harbor several secondary endosymbionts, which are maternally inherited from mother to offspring, that have major effects on host preferences, biology, and evolution. Here, we identified Wolbachia bacteria in sweetpotato whitefly (Bemisia tabaci) as well as whitefly popluations from other countries by comparison of 16S rDNA sequences. Wolbachia were detected in all tested indigenous B. tabaci populations (Bangladesh, Myanmar, Nepal, and the Philippines) as well as Q1 biotype of Korea, whereas they were absent from B biotype of Korea and Q biotype of China. Wolbachia were also detected in all five tested Aleurodicus dispersus populations as well as Tetraleurodes acaciae, whereas they were not detected in all seven Trialeurodes vaporariorum populatuions. In addiiton, Wolbachia were detected in parasitic wasp (Encarsia formosa) of B. tabaci as well as honeybee (Apis mellifera). Among the 19 whitefly populations from different countries, our analysis identified four phylogenetic groups of Wolbachia, thereby demonstrating the high diversity of this genus. Wolbachia phylogeny suggests a correlation of geographical range with ecological variation at the species level.