Extensive soft tissue defects involving loss of skin, fat, and muscle often result from trauma or tumor resection. Current treatments, including autografts and flaps, are limited by donor-site morbidity and scarce tissue availability. Animal models, particularly in rodents, are essential for research but are limited by their primary healing mechanism—contraction via the panniculus carnosus—which does not accurately reflect human healing. Furthermore, standardized models for complex skin–muscle defects are lacking. Therefore, this study aims to create a clinically relevant composite soft tissue defect model in mice using a three-dimensional (3D) polylactic acid (PLA) chimney splint to inhibit contraction and better mimic human wound healing mechanisms (re-epithelialization and granulation tissue formation). A composite defect was created on the dorsum of 8-week-old BALB/c nude mice. The biocompatibility of the 3D-printed PLA chimney was assessed via MTT assay. In vivo, fixation methods—tissue adhesive (TA), simple interrupted sutures (SI), and purse-string suture (PS)—were compared. Wound healing was evaluated over 4 weeks via gross and histological analyses. PLA material showed excellent biocompatibility in vitro, with cell viability consistently above 85%, indicating noncytotoxicity. In vivo, the TA and SI groups showed severe inflammation, tissue necrosis, and splint detachment. In contrast, the PS group remained stable for 4 weeks with no complications. Histologically, the PS group effectively suppressed contraction. Re-epithelialization from the wound edge, well-organized granulation tissue with active angiogenesis, abundant fibroblasts, and collagen deposition, and spindle-shaped cells were clearly observed. In conclusion, this study establishes a reproducible and stable murine composite soft tissue defect model by combining a 3D-printed chimney splint with a PS technique. This model overcomes a key limitation of rodent wound models by controlling contraction, offering a robust preclinical platform to study composite tissue healing and evaluate next-generation regenerative medicine therapies.

This review examines the microstructural and mechanical properties of a Ti-6Al-4V alloy produced by wrought processing and powder metallurgy (PM), specifically laser powder bed fusion (LPBF) and hot isostatic pressing. Wrought methods, such as forging and rolling, create equiaxed alpha (α) and beta (β) grain structures with balanced properties, which are ideal for fatigue resistance. In contrast, PM methods, particularly LPBF, often yield a martensitic α′ structure with high microhardness, enabling complex geometries but requiring post-processing to improve its properties and reduce stress. The study evaluated the effects of processing parameters on grain size, phase distribution, and material characteristics, guiding the choice of fabrication techniques for optimizing Ti-6Al-4V performance in aerospace, biomedical, and automotive applications. The analysis emphasizes tailored processing to meet advanced engineering demands.

Background: The forward head posture acts as a factor that can cause various neurovascular and musculoskeletal dysfunctions. But searching for a study on quality of life for patient with forward head posture was challenging. Therefore, this study aims to find the factors that most affect the quality of life in patients with forward head posture. Objectives: The purpose of this study was to investigate the correlations between the cranio-vertebral angle (CVA), neck disability index (NDI), pain, and sternocleidomastoid (SCM) thickness of patients with forward head posture and the quality of life of the patients and to figure out important factors that affect the quality of life of the patients with forward head posture. Design: Cress-sectional study. Methods: To measure the CVA, the angle at which the visible protrusion of C7 and the ear bead were connected was measured, and the neck disorder index was evaluated using the Korean version of NDI. The degree of pain of the subject was measured using a visual-analog scale (VAS). The SCM thickness was measured using an ultrasound imaging device, and the quality of life was evaluated using the Korean version of the World Health Organization quality of life questionnaire (WHOQL-BREF). Results: A significant predictive model showing 88% explanatory power for the dependent variable was confirmed, with an appropriate regression equation being found. The factor that most affected patients' quality of life in the forward head posture was confirmed by the SCM thickness. Conclusion: When applying an intervention to improve a patient's quality of life for patient with forward head posture, an intervention method that improves the SCM thickness should be recommended.

한국에서 알스트로메리아(Alstroemeria)는 저온 작물로 난 방비 절감 효과가 있고 장식용 절화 소재로 꾸준히 소비되어, 일정 재배면적이 유지되고 있다. 본 연구는 알스트로메리아 ‘한에로스’의 기내 대량증식과 뿌리 발근 조건을 구명하고 무 균묘 생산 시스템을 구축하기 위해 수행했다. 기내 대량 증식 조건 구명 실험에는 Murashige and Skoog(MS) 배지(3% sucrose, 0.25% gelrite)에 BA 단용 처리(BA 0.25, 0.5, 0.75mg･L-1)와 NAA 0.2mg･L-1에 BA를 농도(BA 0.25, 0.5, 0.75mg･L-1)에 따라 혼용 처리하였다. 발근 조건 구명을 위 해 MS배지(3% sucrose, 0.25% gelrite)에 BA 0.25 mg･L-1 단용 처리와 BA 0.25mg･L-1에 NAA를 농도(NAA 0.1, 0.2, 0.3mg･L-1)에 따라 혼용 처리하였다. 뿌리줄기 증식 실험 결 과, 호르몬 첨가에 따른 생육차이는 뿌리줄기 수를 제외한 나 머지 부분에서 뚜렷하게 확인되었다. 단용 처리구 보다 혼용 처리구에서 많은 신초가 유도되었다. BA 0.5mg･L-1와 NAA 0.2mg･L-1 혼용 배지에서 가장 많은 1.43개의 신초가 유도되 었다. 발근 실험 결과, ‘한에로스’는 BA 0.25mg･L-1 와 NAA 0.3mg･L-1 혼용 배지에서 뿌리 수가 3.96개로 가장 많고, 뿌 리가 짧고 두꺼워 순화 과정 중에 뿌리 손실이 적었다. 또한, 순화 과정 중 묘가 고사하지 않았다. 종합적으로, ‘한에로스’ 는 BA 0.5mg･L-1와 NAA0.2 mg･L-1 혼용 배지에서 증식을 하는 것이 적절하며, BA 0.25mg･L-1와 NAA 0.3mg･L-1 혼 용 배지에서 발근 후 순화하는 것이 최적의 기내 배양 조건으 로 사료된다. 본 연구의 결과로 알스트로메리아 무균묘의 보 급 확대에 기여하고자 한다.

Emotional labor, characterized by a dysfunctional type of emotional regulation called surface acting, has detrimental psychological consequences on employees, including depression and social anxiety. Because such disorders exhibit psychological characteristics manifested through brain activation, previous studies have succeeded in distinguishing individuals with depression and social anxiety from healthy controls using their functional connectivity characteristics. However, it has not been established whether the functional connectivity characteristics associated with emotional labor are distinguishable. Thus, we obtained resting-state fMRI data from participants in the emotion labor (EL) group and control (CTRL) group, and we subjected their whole-brain functional connectivity matrices to a linear support vector machine classifier. Our analysis revealed that the EL and CTRL groups could be successfully distinguished on the basis of individuals' connectivity patterns, and confidence in the classification was correlated with the scores on the depression and social anxiety scales. These results are expected to provide insight on the neurobiological characteristics of emotional labor and enable the sorting of employees undergoing adverse emotional labor utilizing neurobiological observations.

The CC chemokine receptor 5 (CCR5) is a G protein-coupled receptor that regulates chemotaxis and effector functions of immune cells. It also serves as the major co-receptor for the entry of human immunodeficiency virus (HIV). Recently, CCR5 inhibitors have been developed and used for the treatment or prevention of HIV infections. Additionally, it has been identified that CCR5 controls bone homeostasis by regulating osteoclastogenesis and the communication between osteoblasts and osteoclasts. However, the effects of CCR5 inhibition on bone tissue in elderly patients are unknown. This study aimed to examine the bone phenotype of aged CCR5 knockout (KO) mice. Femoral and tibial bones were isolated from 12-month and 18-month old wild-type (WT) and CCR5 KO mice, and microcomputed tomography and histology analyses were performed. Twelve-month-old CCR5 KO mice exhibited a decreased trabecular bone mass and cortical bone thickness in both femoral and tibial bones compared with agematched WT mice. Eighteen-month-old mice also showed a decreased trabecular bone mass in femurs compared with control WT mice, but not in tibial bones. Unlike in 12-month-old mice, the cortical margin of femurs and tibias in 18-month-old mice were rough, likely because they were aggravated by the deficiency of CCR5. Overall, our data suggest that the deficiency of CCR5 with aging can cause severe bone loss. When CCR5 inhibitors or CCR5 inactivating technologies are used in elderly patients, a preventive strategy for bone loss should be considered.