The purpose of this study was to develop the best mushroom cultivation conditions and the combination of mushroom culture media in order for mushroom producers and consumers. To reach this target, we first investigated the genetic relationship and developed suitable conditions of mycelial growth in Hypsizygus marmoreus strains. One superior strain of H. marmoreus was selected from 124 strains using bag culture. One hundred and twenty four strains were genetically classified into four main groups using two Universal Rice primers, URP2R and URP17R. The studies on the effects of different temperature (17, 21, 25, 29, 33℃) showed that 25℃ is the best temperature for mycelial growth for almost all strains while at 33℃ most of mycelium stop growth. Finally, ten strains were selected according to the groups identified by their temperature requirements. The length of mycelial growth in PDA, MCM, GPYM, MEA and MYP were longer than those in Czapek Dox. The selected ten strains of H. marmoreus showed heavier dry weight of mycelia at pH 3.0∼7.0 than any other pH. Although it was not show distinct requirement of carbon and nitrogen sources for vegetative growth according to strains, mainly the mycelial growth of the ten selected strains were observed at media including xylan and yeast extract, peptone, tryptone, respectively. Moreover, higher C/N ratio was observed in higher dry weight of mycelia.

For the development of SSR marker system in Vicia villosa Roth, an enriched library was constructed by using a modified biotin-streptavidin capture method and the selected clones were sequenced with GS-FLX(454). Of 37,794 sequenced reads, we found that 8,474 reads (22.4%) were redundant, leaving 29,320 unique ones (77.6%). Among the unique clones, 17,174 reads (58.6%) were having microsatellite repeating motifs. Sequence analysis of all SSR-containing reads revealed a predominance of the di-nucleotide SSRs (62.5%). The tri-nucleotide and the tetra-nucleotide SSRs were 5.7% and 22.5%, respectively. As the di-nucleotide type, the AG/GA class of repeat motif was most frequently identified (55.0% of the total di-nucleotide SSRs), followed by the CT/TC class (19.5%), and the TA/AT class (12.1%). Among the tri-nucleotide SSRs, the AGT/GTA/TAG class of repeat motifs was predominant (22.2%), followed by the ACT/CTA/TAC class (17.8%). Among the tetra-nucleotide SSRs, the CTTT/TTTC/TTCT/TCTT class of repeat motifs was predominant (31.2%), followed by the AAAG/AAGA/AGAA/ GAAA class (19.9%). Finally, we designed 779 primer pairs from the flanking sequences of SSR containing reads. We are undertaking the analysis of polymorphisms using the diverse collected accessions of Vicia villosa Roth now. This newly developed SSR marker set shall provide a very useful tool for implementing molecular diversity assessment and population structure studies of Vicia villosa Roth onward.