Abstract Purpose : The aim of this study was to identify knowledge and lifestyle habits for glaucoma prevention and to present basic data for future glaucoma prevention education. Methods : From April to September 2023, 447 people in their 20s to 60s were selected and an online survey was conducted. The questionnaire consisted of a total of 30 questions, including 6 questions about the subject's general characteristics, 14 questions about knowledge and awareness of glaucoma, and 10 questions about lifestyle habits related to intraocular pressure. Results : 89.7% responded that they had heard of glaucoma, and 31.1% of respondents knew the difference between glaucoma and cataracts. 34.5% responded that early detection is difficult, 33.3% that complete treatment is difficult, 23.5% that even normal intraocular pressure can cause glaucoma, and 36.0% that the incidence of glaucoma is increasing day by day. The level of awareness and knowledge was higher with age, in women than men, in those with a family history, in professional occupations, with metabolic diseases, and with high myopia. Lifestyle habits related to intraocular pressure were higher with age, among women, when smartphone use was less than 3 hours, and among housewives and professionals. The relationship between glaucoma knowledge and awareness and intraocular pressure-related lifestyle habits was statistically significant (r=0.248, p<0.001). Conclusion : Although the level of basic knowledge about glaucoma was high, the level of specific knowledge was low compared to the increase in the incidence of glaucoma over the past 10 years. Key words : Glaucoma, Healthy Life Practices, Intraocular pressure