Propolis, bee glue is a sticky substances, which is used to prevent corruption of the ones who broke into the hive, and which is known as a substance that inhibits the growth of microorganisms within the hive. In order to use this propolis, is required to extract the active ingredients from the raw propolis. Mainly goes through the process of extraction with ethanol. Propolis is registered as health functional food for food and drug safety with a valid registration which is anti-oxidant effects and anti microbial effects of oralflora. Inthis study, in order to determine the antioxidant effect of propolis produced in various regions collected 58 regions. Antioxidant effects were tested by DPPH free radical scavenging effects method. Asa result, the propolis concentrations by 10, 50, 100, 500, 1000㎍ were 43, 73, 78, 82, and 73%, in Gangwon province, Gyeonggi Province, 57, 83, 83, 79, and 71%, Chungcheong Province, 55, 80, 80, 76, and 68%, Jeolla province 48, 75, 78, 78, and 71%, Gyeongsang province 47, 77, 81, 79, and 71%, respectively. More than a certain concentration was to determine antioxidant falling rather. These results can be said to suggest if you use the edible excess should be avoided.