The trophectoderm is one of the earliest cell types to differentiate in the forming placenta. It is an important for the initial implantation and placentation during pregnancy. Trophoblast stem cells (TBSCs) develop from the blastocyst and are maintained by signals emanating from the inner cell mass. However, several limitations including rarity and difficulty in isolation of trophoblast stem cells derived from blastocyst still exist. To establish a model for trophoblast differentiation, we isolated TBSCs from human term placenta (38 weeks) and characterized. Cell cycle was analyzed by measuring DNA content by FACS analysis and phenotype of TBSCs was characterized by RT-PCR and FACS analysis. TBSCs have expressed various markers such as self-renewal markers (Nanog, Sox2), three germ layer markers (hNF68, alpha-cardiac actin, hAFP), trophoblast specific markers (CDX-2, CK7, HLA-G), and TERT gene. In FACS analysis, TBSCs isolated from term placenta showed that the majority of cells expressed CD13, CD44, CD90, CD95, CD105, HLA-ABC, cytokeratin 7, and HLA-G. Testing for CD31, CD34, CD45, CD71, vimentin and HLA-DR were negative. TBSCs were shown to decrease the growth rate when cultured in conditioned medium without FGF4/heparin as well as the morphology was changed to a characteristic giant cell with a large cytoplasm and nucleus. In invasion assay, TBSCs isolated from term placenta showed invasion activities in in vivo using nude mice and in vitro Matrigel system. Taken together, these results support that an isolation potential of TBSCs from term placenta as well as a good source for understanding of the infertility mechanism.

To find out the best complex fertilizer for high yielding of rapeseed crop, experiment was conducted on complex fertilizers at the experiment field in upland of rapeseed in Mokpo Experiment Station, Nat'l Institute of Corp Science, RDA, Korea. Experiment was laid out in randomized complete block (RCBD) design. The effects of complex fertilizer (22-22-11) on the number of branches, pod length, percentage of seed set and seed yield were highest but on the plant height, ear length, and number of pods per ear were negligible. On th basis of the results reported above, for getting higher yield of rapeseed crop, among the tested fertilizers complex fertilizer (22-22-11) gave the superior performance and is recommended for application.

This study was conducted to evaluate the effect of seed disinfectant, in control of brown leaf blight, growth characteristics, and dry root yield in the cultivation of Alisma plantago after early maturing rice cropping. Experimental plot was laid out in split plots design with three replications. The major seed disinfectants were benomyl Wp, 20%, Captan Wp, 50%, Triferine Ec, 17%, Etridia zole Ec, 25%, and Thioplant-mythyl Wp, 50%. Even though seed disinfectant treated had no effect on the growth and flowering date of Alisma plantago, dry root yield was increased largely with benomyl Wp, 20%, in seed disinfectant than in the other seed disinfectants and contorl. All seed disinfectants had no injury with standard dosage. But all seed disinfectants had slight injury in the double dosage level for the Alisma plantago. On the basis of yield, vegetative and disease paramerer, benomyl Wp (20%) (100g/20l) had shown superior performance, however, all the seed disinfectants are effective as compare to without treatment.