An increased use of organic phosphorous based chemical pesticides in domestic and overseas agriculture has resultedin emergence of tolerant strains of insect pests. These pests have spread throughout South Korea and are responsible forsignificant reduction in crop yields. Furthermore, as yet there is no effective way of controlling these insects or the damageresulting from pest infestation. To this end, we are attempting to develop biological control based methods to protectcrop plants against insect pests. A screen for biocontrol agents conducted in five farms that were heavily infested bynematodes in 2015-2016 identified Aspergillus niger F22 strain that was very effective against root-knot nematode. Afour-time treatment with A. niger F22 at an internal of ten days was able to reduce nematode population by 70-90%compared to untreated controls. The biocontrol agent was also more effective compared to ograno-phosphorus based pesticide,which caused a 30% reduction in nematode population. Notably, the extract (suspension concentrate) prepared from A.niger culture was equally effective in controlling the root-knot nematode and is currently being developed as a marketableproduct under the brand name “NAMAFREE”.

Thrips worms and root-knot nematodes occur in a variety of crops, and have shown a great deal of damage to farm income every year, and the damage is increasing every year. In order to solve these problems, a variety of biological materials are used in Korea to develop a control agent. However, there are very few products available that can satisfy the consumer's satisfactory control effect, efficacy, formulation stability and pesticide compatibility. In order to propose a biological control solution to these problems, this study was conducted to develop the optimal bioprocess technology and formulations suitable for the material by transferring the Aspergillus nigerF22 strain, which is effective for root-knot nematodes, at Chonnam National University. This study was conducted to investigate the efficacy of Aspergillus niger F22 20% suspension concentrate (Productname:NEMAFREE), which has excellent efficacy on root nematodes. The packing test result showed about 70-90% control effect. Soil fumigation and disinfection treatments after 4 days of planting were effective. In addition, we have developed a product to control the under powder pupa using Beauveria bassiana ERL836, an insect pathogenic microorganism, which has excellent control effect against resistant insect pupa. The main purpose of this study was to investigate the effect of insect pests on the under poor control of the pupa in the soil. In the pavement test, more than 70%(GR) formulation, which can be treated withch emical pesticides, and it is confirmed that synergy effect is in the control of Thrips worm.

Globally, there is an increase in demand for organic products which has boosted the organic food industry. There has been a continuous incease in th organic agricultural land due to the aforementioned reaseons which has been in fluencing the use of chemical-free pesticides, fertilizers, and other agricultural inputs for cultivation. Also, the increasing per-capita expenditure income and high purchasing power of consumers in developing countries such as India, Brazil, South Africa, Korea, Japan, and China have forced the manufactures to offer a wide range of organic products to sustain competition. Agricultural biologicals are a group of products which are derived from natural sources such as microorganisms, plant extracts. or other biological matter, which can be used as a substitute for agriculture chemical products. Farmers can depend on these agricultural biologicals along with the biotechnology and variety of agronomic practices, in order to grow healthy and nutritious crops.