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        검색결과 2

        1.
        2004.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        KISTI(한국과학기술정보연구원)는 학회 업무 관리, 학술정보 관리를 학회가 어려움 없이 처리하도록 지원하고자, 학회 정보화 사업의 일환으로 학회 학술정보 유통체제 전 과정을 온라인화하고 쉽게 관리할 수 있도록 한 『KISTI-ACOMS(KISTI-Article COntribution Management System: KISTI-학회논문투고관리시스템)』을 개발하여 2001년부터 학회에 무상으로 보급하여 왔다. 최근 KISTI-ACOMS를 이용하고자 하는 학회의 요청이 더욱 확대되고 있어, KISTI는 많은 학회의 특성들을 의견수렴하여, KISTI-ACOMS의 표준화를 목표로 보완해 나가고 있다. 본 연구는 KISTI-ACOMS를 기반으로 구축한 한국환경생태학회의 학회논문투고관리시스템의 기능에 대해 살펴보고, 향후 시스템에 필요한 기능 및 명세를 기술함으로써 한국환경생태학회의 개선된 온라인논문투고관리시스템을 제시한다.
        4,000원
        2.
        2014.09 KCI 등재 서비스 종료(열람 제한)
        Adaptive development of early stage embryo is well established and recently it is explored that the mammalian embryos also have adaptive ability to the stressful environment. However, the mechanisms are largely unknown. In this study, to evaluate the possible role of aquaporin in early embryo developmental adaptation, the expression of aquaporin (AQP) 5 gene which is detected during early development were examined by the environmental condition. To compare expression patterns between in vivo and in vitro, we conducted quantitative RT-PCR and analyzed localization of the AQP5 by whole mount immunofluorescence. At in vivo condition, Aqp5 expressed in oocyte and in all the stages of preimplantation embryo. It showed peak at 2-cell stage and decreased continuously until morula stage. At in vitro condition, Aqp5 expression pattern was similar with in vivo embryos. It expressed both at embryonic genome activation phase and second mid-preimplantation gene activation phase, but the fold changes were modified between in vivo embryos and in vitro embryos. During in vivo development, AQP5 was mainly localized in apical membrane of blastomeres of 4-cell and 8-cell stage embryos, and then it was localized in cytoplasm. However, the main localization area of AQP5 was dramatically shifted after 8-cell stage from cytoplasm to nucleus by in vitro development. Those results explore the modification of Aqp5 expression levels and location of its final products by in vitro culture. It suggests that expression of Aqp5 and the roles of AQP5 in homeostasis can be modulated by in vitro culture, and that early stage embryos can develop successfully by themselves adapting to their condition through modulation of the specific gene expression and localization.