The purpose of this study was to investigate the warming temperature and exposed time on the post-thaw survival rate and viability of bovine blastocyst cryopreserved by GMP vitrification. Groups of three bovine IVP blastocysts were sequentially placed into vitrification solution before being loaded into the GMP straws and immersed into LNwithin 20 to 25 sec. The warming rate was increased 2 times of warming temperature for improvement of post-thaw survival rates. The frozen embryos were warmed either at 35 or 70 for 1 or 2 sec and then diluted in sucrose solution. Post-thaw blastocysts were serially washed in 0.25 and 0.15 M sucrose in holding medium (HM: TCM199 supplemented with 10% FCS) and TCM-199 for each 5 min, respectively, and then cultured in TCM199 for 24 h. The rate of re-expanded blastocyst was significantly different fer 35 and 70 warming temporature (76.4 vs. 89.3%; P<0.05). The rate of re-expanded blastocyst at 70 for 1 sec was significantly higher than that for 2 sec (91.1 vs. 70.9%; P<0.05). The number of nuclei counted were significantly different among control, 35 and 70 (1218.5 vs. 10411.7 vs. 11410.3; P<0.05). These results indicated that the increasing of warming rate can provide high survival rates of bovine IVP blastocysts. Especially, the best viability of post-thaw blastocyst could be thaw at 70 for 1 sec. The warming temperature and exposed time far warming was considered to be limiting factors to the viability of bovine IVP embryos. he purpose of this study was to investigate the warming temperature and expose.

32 P 표지 중과석과 칼슘으로 대두에 있어서 밭토양별 인산흡수계수에 의한 인산질 비료와 석회의 합리적인 시용량을 알고자 본시험이 수행되었다. 1 ) 석회의 시용은 기개간지, 미개간지 공히 생육 및 수량을 좋게 하였다. 2) 인산시용은 기개간지는 인산흡수계수 5% 미개간지는 15%에서 좋은 경향을 보였으며 석회와 인산간의 상호관계는 인정할 수 없었다. 3) 32 P 표지 중과석에 의한 시용인산의 기여도는 기개간지는 미개간지보다 다소 높은 수치를 나타내었다. 4) 기여도를 산출한 결까 미개간지는 석회시용구구(P320.2~0.3miu ) 14~16%가 석회무시용구 14~17%로 근소한 차이를 보였다. 5) 기개간지에서는 석회시용구(32 P 0.2~0.3 miu ) 21%가 석회무시용구 14~17%에 비해 높은 경향을 보 였다.