Lentinula edodes, a member of the Pleurotaceae, is a blood pressure and cholesterol lowering effect. Recently, research the reduction of irritation and the enhancement of function have been cultivation of natural resources using mushroom mycelium. For the development of food fermented with L. edodes mycelium of natural medicinal herb remnants, determined to be 20%, and the product were used after lyophilization. In this study, we were analyzed the contents of β-glucan and amino acids of fermented herb remnants by L. edoeds mycelials. β -Glucan is a type of polysaccharide that has immune-enhancing and is known to inhibit cancer cells. The content of β-glucan and total amino acids of fermented herb remnants by L. edoeds mycelials were 35.13% and 8,697.36 mg%, respectively. The content of essential amino acid from fermented herb remnants by L. edoeds mycelials was 3,226.85 mg%. Collectively, our findings suggest that fermented herb remnants by L. edoeds mycelials extract could be used as an chemo-preventive material for consumers.

β-glucan is a safe and highly potent biological response modifier that nutritionally activates the immune response through the Macrophage, Dendritic and additional immune cells to yield various therapeutic effects. Shiitake mushrooms (Lentinula edodes) contanining β-glucans may be beneficial for human health; they have been used in the treatment of cancer, hypertension, and high cholesterol levels. The effect of different substrates and various developmental stages (mycelium growth, primordium appearance, and fruiting-body formation) on β-glucan production in the edible mushroom L. edodes was studied. The cap of the mature mushroom showed the highest β-glucan activity, and β-glucan activity seemed to be influenced somewhat by some well-known inducers or sawdust. In this study, we utilized five strains (JMI 10022, 10036, 10077, 10079, 10080) of L. edodes regardless of origin and growth conditions. This experiment showed that the expression of β-glucan was induced by glucose bond, and increased with the growing of L. edodes. Quantitatively, reverse transcription PCR utilized pairs of primers specific for β-glucan gene expression shows that expressed genes were most commonly indentified during the process of fruiting-body formation. We suggest that the results will provide valuable information to assist L. edodes industry.

Recently, hair loss regarded as a mere means of middle-aged men due to stress and environmental pollution. The market for hair loss in Korea is about four trillion won and it is growing continuously. It is mainly made by mixing natural extracts such as medicinal plant. The purpose of this study was to investigate the effects of 70% ethanol extracts of Phellinus linteus on the growth of fibroblasts, dermal papilla cells and lipid precursors, We want to try to make a materialization. The 70% ethanol extract of P. linteus on the cell viability of Human follicle dermal papilla cell (HFDPC), adipocyte (3T3-L1), fibroblast (CCD-986sk). The result the cell viability of HFDPC was increased according to the contents of ethanol extract of P. linteus. But the 70% ethanol extract of P. linteus showed weak inhibit activity in CCD-986sk cell growth. Also, the 70%　ethanol extract of P. linteus did not inhibit the growth of 3T3-L1 cells. As a result of this study, in-vitro hair growth activity of herbal medicines for hair material development was not shown to be toxic to HFDPC and 3T3-L1 cell. As a result, the 70% ethanol extracts of P. linteus have potential to developing hair-related product. Acknowledgement : This research was supported by the Ministry of Trade, Industry & Energy(MOTIE), Korea Institute for Advancement of Technology(KIAT) through the Encouragement Program for The Industries of Economic Cooperation Region(R0005473)