Italian ryegrass (Lolium multiflorum Lam., IRG) is a widely cultivated winter forage crop known for its high yield and nutritional value. This study evaluated the processing characteristics and feeding performance of IRG-based pellets in Hanwoo cattle (Bos taurus coreanae) and Korean native black goats (Capra hircus). IRG was harvested at the optimal growth stage and processed into two pellet formulations: IRG ≥80% (with up to 20% soybean meal) and 100% IRG. Feeding trials were conducted under ad libitum feeding conditions. Hanwoo cattle showed higher intake of 100% IRG pellets (7.9 kg/day/head) than IRG ≥80% pellets (7.5 kg/day/head, p<0.05), with similar average daily gain (0.9 ± 0.4 kg/day/head). Conversely, black goats exhibited significantly lower intake of IRG ≥80% pellets (54.6 g/day/head) compared to 100% IRG pellets (266 g/day/head), likely due to reduced palatability associated with soybean meal inclusion. These findings suggest that IRG pellets are suitable for Hanwoo cattle, while further optimization of pellet size and formulation is required to improve acceptance in goats. Future studies should assess long-term impacts on digestion, rumen fermentation, and metabolic responses.

Occurrence spontaneous mushrooms in eastern Jeju Muryeongarioreum, Sillyecheon-Iseungak, Kyoraegotjawal, Geomunoreum were examined in 2016. The collected mushrooms were classified by 15 order 56 family 149 genera 317 species. Among the collected mushrooms, 87 species of mushrooms were successfully incubated in mushroom complete medium in which DNA of 25 species were completely extracted and amplified using universal primers ITS1 (5’-CTTG GTCA TTTA GAGG AAGT AA-3’) and ITS4 (5’-TCCT CCGC TTAT TGAT ATGC-3’). Four species of them were differently identified between the classification by morphological observation and by analysis of DNA sequences i.e. by morphological identification Ganoderma applanatum, Armillaria gallica, Macrolepiota procera and Trametes gibbosa were identified as G. gibbosum, A. cepistipes, M. detersa and T. elegans by analysis of DNA sequences, respectively. Based on these results both identification ways might be necessary for correct classification of mushrooms. On the other hand, among the collected 317 mushrooms, 48 species could be identified to genus but not to species by the morphological observations. However, by the analysis of DNA sequences, 15 species were identified to species in which 6 species were unregistered mushrooms in Korea. Therefore, the analysis of DNA sequence may be useful to identify the mushrooms which are difficult to recognize by morphological observation.