Dysmicoccus brevipes (Cockerell), Dysmicoccus neobrevipes Beardsley and Pseudococcus longispinus (Targioni-Tozzetti) are widely-distributed pests that feeds on many economically important hosts, particularly tropical fruits and ornamentals. The potential distribution of these mealybug pests into South Korea remains a primary concern because of their high incidence in interceptions screened during inspection. Hence, these species prompted a modelling effort to assess their potential risk of introduction. Potential risk maps were developed for these pests with the CLIMEX model based on occurrence records under environmental data. The potential distribution of these pests in South Korea in the 2020s, 2050s and 2090s was projected based on the RCP 8.5 climate change scenario. Results show that D. brevipes, D. neobrevipes and P. longispinus have little potential for invasion in the exterior environment of South Korea due to high cold stress in the 2020s. However, for D. brevipes and P. longispinus, 3 and 86 locations in Jejudo, were predicted to be marginally suitable for this pest under future climate factors, respectively. In that respect, the results of these model predictions could be used to prepare a risk-based surveying program that improves the probability of detecting early D. brevipe, D. neobrevipes and P. longispinus populations.

Objective : Dementia prevention program has been applied in Center for dementia by the occupational therapists. We evaluated the efficacy of this program to the elderly non-demented women in senior citizens center for cognitive, physical function and depression.Methods : We applied this program to women subjects from a senior citizens centers in 12 sessions. Cognitive and physical functions and depression were compared between pre and post-intervention with Korean Mini-Mental State Examination (K-MMSE), Timed-Up and Go test (TUG), One Leg Standing Test (OLST) and Geriatric Depression Scale Short Form-Korea version (GDSSF-K).Results : We enrolled 26 non-dementia women from four senior citizens centers in Seoul Yangcheon-gu. The K-MMSE improved after the dementia prevention program significantly (p=.00). The GDSSF-K was significantly different between pre and post-intervention (p=.02). For physical function, TUG (p=.02) and OLST in opening eyes were significantly improved (respectively, p=.00 in right side, and p=.00 in left side). OLST in closed eyes were tend to be improved after the intervention, however, they were not significantly different.Conclusion : The dementia prevention program of Center for dementia has proved to improve cognitive and physical function and to reduce depression in the non-demented women from senior citizens centers. These findings suggest that the dementia prevention program that has led by occupational therapist in Center for dementia can be applied other setting in a community.

Cryopreservation and in vitro fertilization (IVF) protocols are important in genetic studies and applications to transgenic animals. Various studies about boar sperm cryopreservation have been studied for a long time. Those were about the use of extenders, the choice of sugars, the cooling and warming rates. The factors that influence the boar sperm are the dramatic changes in temperatures, osmotic and toxic stresses, and reactive oxygen species (ROS) generation. Among these factors, ROS generation is the main damage to DNA which is a principal genetic material and the most important for the practical applications. So we wondered whether ROS generation could be reduced. In previous study, monothioglycerol (MTG) was essential for the culture of embryo stem cells. Therefore we added MTG in the freezing extender based on lactose-egg yolk (LEY) with trehalose. For the assessment of the frozen-thawed sperm, we focused onmotility, membrane integrity and DNA damage. First, we used a computer-aided sperm analysis system for overall conditions of sperm such as motility and viability. Then we performed the sperm chromatin structure assay for DNA integrity and hypo-osmotic swelling test for membrane integrity. And our result showed the existence of MTG in the freezing extender caused less damage to DNA and higher motility in frozen-thawed boar sperm. Also we checked a relative antioxidant activity of MTG in modified Modena B extender. We concluded that this reagent can activate sperm mitochondria at MTG 0.2 μM, contribute to sperm motility and DNA integrity but there was no significant difference on membrane integrity. Also antioxidant activity of MTG in modified Modena B extender was proved.