This paper examined farmers’ perceptions and preferences for improved varietal traits in the Wenchi and Offinso North Municipalities of Ghana. Data from 306 randomly selected tomato farmers were analyzed using perception indices, Kendall’s Coefficient of concordance and the Poisson regression model. The results show that tomato farmers are willing to adopt an improved variety with long shelf life, good fruit quality and large fruit size. The study further indicates that tomato farmers lack requisite skills in pest and disease management. The number of varietal attributes preferred by farmers was positively influenced by sex of farmer, education, experience in tomato cultivation, household size, access to credit, FBO membership, extension contacts farm size and off-farm income. These factors and attributes need to be carefully considered by breeders and policy makers in the development of an improved tomato variety to enhance its uptake. The major constraints identified in tomato production include limited access to capital followed by low commodity prices coupled with low demand with the least constraint being access to tractors for land preparation. The potential of adoption of improved tomato varieties in Ghana will be augmented through paragenetic measures towards addressing these constraints.