Carbonyl sulfide(COS) is a naturally generated gas from fermentation process of microbes and from plant root and stem. COS was firstly registered as a fumigant at 1993 to control stored product pests. To supplement environmental problems and toxicity of commercial fumigants and develop new fumigant, we have processed the susceptibility assessment of carbonyl sulfide on important agricultural pests, Myzus persicae and Tetranychus urticae. Every growth stages of two insect species were tested, and five dosages of carbonyl sulfide were treated for 4 hours, and the mortality was investigated after 24 hours of treatment. Nymphal stage of M. persicae was completely controlled at more than 20 mg/L dosage, and adult stage showed 95.8% mortality at 80 mg/L dosage. The LC50 of M. persicae was 7.314mg/L for nymph and 26.117mg/L for adult stage. Egg stage of T. urticae showed 91.2% mortality when treated with 100mg/L carbonyl sulfide, and nymph and adult stage showed 100% and 94.1% mortality at 8mg/L and 80mg/L, respectively. The LC50 of T. urticae was 73.110mg/L for egg, 2.818mg/L for nymph and 12.054mg/L for adult stage.

Phosphine (PH3) resistance in the stored-products insect pests has been reported throughout the world in various insect species, including Rhyzopertha dominica, Tribolium castaneum, and Cryptolestes ferrugineus, leading farmers and fumigators to identify new fumigation tools to control PH3-resistant insect pests in storage facilities. Understanding PH3-resistance mechanisms in insects might contribute to providing clues for the development of new chemicals, including fumigants, to control various PH3-resistant insects. A proteomic study has shown 15 decreased proteins in the PH3-resistant R. dominica (CRD343 strain) in comparison to the PH3-susceptible R. dominica, and among those 15 proteins, dihydrolipoamide dehydrogenase (DLD), a protein involved in the Krebs cycle, was identified (Park et al., 2008). The DLD polymorphisms responsible for genetic resistance have disulfide active sites for PH3 binding and are highly sensitive to arsenic exposure after mutagenesis in insects (R. dominica and T. castaneum) and Caenorhabditis elegans (Schlipalius et al., 2012). Here, two PH3- resistant S. oryzae strains were used to understand the development of PH3 resistance in these insects. Acute toxicity test by PH3 on the two PH3-resistant strains was undertaken followed by ethyl formate inhibition study on cytochrome c oxidase activity. The Lineweaver-Burk plots after inhibition studies showed there were significantly difference in inhibition mode between the resistant strains and the control. The RT-qPCR analysis and the next-generation sequencing of the mitochondrial DNA revealed significant changes in metabolism and energy production. Taken together, the PH3 resistance in S. oryzae was definitely acquired by the overall transformation of biochemical reactions to overcome PH3 toxicity.

A growing interest in ecotoxicological study is on the development of tools and technical resources to assess or determine the effects of a variety of stresses on ecosystems. As many chemicals are synthesized and used for the various purposes, it is inevitable circumstance for organisms to meet the stressors in the environment. Thus, it is important for us to understand the impacts of the stressors to organisms and is essential to equip with a fast detecting system to predict of their behaviors. The high throughput technology using proteomic and metabolomic techniques has been introduced to satisfy such requirements to study the potential adverse effects of some chemicals. Ecotoxicproteomics and Ecotoxicometabolomics are discussed in this talk in terms of their possible role in ecotoxicological studies.

The purpose of this study was to investigate the effects of the increasing ankle range motion program on ambulation and balance for the elderly with balance disorder. Eighteen elderly subjects were administered with a timed test twice; approximately 4 weeks apart. The exercise group participated in a fall prevention exercise class at the Y.S. Senior Welfare Center of the Seoul Metropolitan Government. The session consisted of a stationary cycle, static stretching ankle joints, balance boards, and progressive resistive exercises using the Thera-band. The results were as follows: Firstly, the increasing ankle range of motion program was effective on the exercise group. Gait-speed was improved (p<.005). Secondly, balance was significantly improved (p<.000). Thirdly, increasing ankle range of motion program was effective in ankle dorsiflexion which was the major risk factor for falls among the elderly. Differences in gait and balance between the groups were examined using a paired t-test (p<.05). The exercise group demonstrated significantly higher values of gait-speed, ankle range of motion, and balance when compared with the non-exercise group. The results of this study suggest that the increasing ankle range of motion program is an effective intervention for the elderly with balance disorder.