An endoparasitoid wasp, Cotesia plutellae parasitized young larval of diamondback moth, Plutella xylostella. Parasitized larva exhibit significant immunosuppression and fail to metamorphose to pupal stage. Especially, during last instar of parasitized P. xylostella, massive nutrients divert from host to wasp development. HTIF (host translation inhibitory factor) encoded in C. Plutella bracovirus (CpBV) play a crucial role in suppressing host usage of amino acids. However, its inhibitory activity is selective by discriminating mRNAs based on their 5’UTR secondary structures. Our RT-PCR and proteomic analysis indicated that arginine kinase mRNA was inhibited by HTIF, but imaginal disc growth factor was not. Arginine kinase and IDGF were persistently expressed in parasitized P. .xylotella with the gradual decrease at the late parasitisation period. Expression of arginine kinase and IDGF were also tissue specific in the gut/epidermis and haemocyte but not in fat bodies. Subsequent analysis of these gene functions by RNA interference explained the benefit of parasitoid for the mRNA discrimination by HTIF.