Background: Despite its anticancer activity, cisplatin exhibits severe testicular toxicity when used in chemotherapy. Owing to its wide application in cancer therapy, the reduction of damage to normal tissue is of imminent clinical need. In this study, we evaluated the effects of catechin hydrate, a natural flavon-3-ol phytochemical, on cisplatin-induced testicular injury. Methods: Type 2 mouse spermatogonia (GC-1 spg cells) were treated with 0-100 μM catechin and cisplatin. Cell survival was estimated using a cell proliferation assay and Ki-67 immunostaining. Apoptosis was assessed via flow cytometry with the Dead Cell Apoptosis assay. To determine the antioxidant effects of catechin hydrate, Nrf2 expression was measured using qPCR and CellROX staining. The anti-inflammatory effects were evaluated by analyzing the gene and protein expression levels of iNOS and COX2 using qPCR and immunoblotting. Results: The 100 μM catechin hydrate treatment did not affect healthy GC-1 spg cells but, prevented cisplatin-induced GC-1 spg cell death via the regulation of anti-oxidants and inflammation-related molecules. In addition, the number of apoptotic cells, cleaved-caspase 3 level, and BAX gene expression levels were significantly reduced by catechin hydrate treatment in a cisplatin-induced GC-1 spg cell death model. In addition, antioxidant and anti-inflammatory marker genes, including Nrf2 , iNOS, and COX2 were significantly downregulated by catechin hydrate treatment in cisplatintreated GC-1 cells. Conclusions: Our study contributes to the opportunity to reintroduce cisplatin into systemic anticancer treatment, with reduced testicular toxicity and restored fertility.

본 연구는 2019년부터 2021년 9월까지 수집된 홀스타인 젖소 19,930두의 자료를 수집하였으며, 3 SD (Standard Deviation, SD) 범위에서 벗어나는 이상치를 제외한, 8,675두를 분석에 이용하였다. 자료분석은 Statistical Analysis System (SAS) 9.4 software를 사용하였고, 뒷유방높이, 뒷유방너비, 유방깊이, 유두길이 및 유두둘레의 실측치를 사용하였다. 그리고 뒷유방높이, 뒷유방너비, 유방깊이, 유두길이 및 유두둘레의 실측치와 산차, 305일 유량, 비유단계 및 착유속도의 환경요인의 효과를 추정하였다. 산차와 유량이 증가함에 따라 뒷유방너비, 유두길이 및 유두둘레의 실측치는 유의적으로 증가하는 것으로 나타났으며(p < 0.0001), 비유단계에 따라서 뒷유방너비 및 유방깊이의 실측치는 비유단계가 증가할수록 유의적으로 감소하는 것으로 나타났다(p < 0.0001). 각 환경효과에 따른 유방형질 실측치의 표현형 상관의 결과에서는 뒷유방너비와 유방깊이 실측치가 산차 및 유량수준에 대해 높은 상관을 나타냈으며, 특히, 유방형질 중 유방깊이 실측치와 산차에 대해 -0.40 ~ -0.67로 높은 음(-)의 상관을 보였다. 본 연구 결과로 미루어 볼 때, 유방형질이 유생산에 밀접한 관련이 있는 만큼 중요한 형질로 취급되어야 하며, 이들의 기초연구와 함께 유전적 특성을 구명하는 등 더 많은 연구가 수행되어야 할 것으로 사료된다.

This study was carried out to improve the performance of sewer trenchless entire repair method by using the multifunctional safety high-pressure hose. Because the flexural strength and flexing modulus of elasticity increased in the range from 60℃ to 80℃ of the setting temperature, the unform quality was secured to satisfy the standard quality and consolidate the strength, by supplying steam to the entire sewer through the safety high-pressure hose simultaneously. After the steam was supplied, the temperature of the condensate water with a large amount occurred , compared with a small amount, was very low. So, the setting time was shorter over than one hour by reducing the unsatisfied cure state and excluding the condensate water. The standard quality increased by approximately 15% at upper, central, and lower points of the same sewer was gained from the test result of CIPP(cured in place pipes) constructed at the optimum setting temperature. The steam and air were supplied evenly to the sewer by controlling the safety high-pressure hose in the tense and relaxed state and by using the exclusive function of condensate water. The availability and safety for the constructed process were achieved.

Urban areas (residential, commercial, transportation), each of the points during the same rainfall in the same area, this study was evaluated by a survey to find out the basic data and the characteristics of non-point pollution on local characteristics was performed. To evaluate and compare a non-point source pollution concentrations. The conclusion of this study was to know the influence of non-point pollutants in initial rainfall. There was a difference during the same point was compared. The pollution levels were differences in the point-specific characteristics, the same point compared by rainfall when rainfall is low, the contaminant concentrations were high. Finally, when compared to the number of rainy days. The shorter the number of rainy days, the non-point pollutants were high.

The sensitivities of PrP Sc detection methods, western blotting (WB), immunohistochemistry (IHC) and protein mis-folding cyclic amplification (PMCA) techniques were compared from brains, spleens and blood of mice challenged with PrP Sc of murine-adapted BSE strain 301C. PrP Sc was detected in the spleen from 30 dpi by IHC and at 60 dpi by WB. At 30 dpi, disease-specific signals of PrP Sc was observed in only two follicles of a single spleen. PrP Sc was detected in spleen at 10 dpi with PMCA after 5 rounds of amplification. Clinical signs were obviously shown from 240 dpi, and coincided with first detection of PrP Sc in brains by WB, IHC and PMCA after one round amplification. In addition, PrP Sc was also detected in blood at 60, 180 and 240 dpi with PMCA after 5 rounds of amplification. The FDC-M1 epitope, which appears in immature FDCs, and PrP Sc were detected in follicles first at 30 dpi, whilst the FDC-M2 epitope of mature FDCs was detected at 60 dpi. More FDC-M2 epitope and PrP Sc were detected in follicles as disease progressed. The CD21/35 epitope is expressed on both FDCs and germinal center B cells. The pattern of CD21/35 expressing cells was similar to but less dominant than that of FDCs.

플라스틱 가소제인 di(2-ethylhexyl)phthalate (DEHP)는 매우 잘 알려진 내분비계 장애물질(endocrine disrupting chemicals; EDCs) 중 하나로, 수컷 설취류와 인간의 생식과 발생 과정에 있어 강력한 항안드로겐성 작용을 하는 것으로 알려져 있다. 본 연구는 사춘기 이전에 수컷 흰쥐를 DEHP에 노출시킴으로써 부속 성기관의 성숙 과정 동안 나타나는 변화를 조사한 것이다. 결과로, DEHP 투여에 의한 체중, 혈중 T 수준, 저정낭과 전립선을 제외한 조직의 무게는 대조군과 비교하여 유의적인 변화가 없었다. 저정낭의 경우, 고농도(200 ㎎/㎏)의 DEHP를 처리한 투여군의 무게가 대조군에 비해 유의하게 감소하였으며(p<0.05), 전립선의 경우, 저농도(20 ㎎/㎏)와 고농도의 DEHP를 처리한 모든 투여군에서 대조군에 비해 유의한 무게의 감소를 나타내었다(p<0.05). 조직학적 연구 결과, DEHP 투여군의 저정낭은 대조군에 비해 점막층의 면적이 감소하였다. 또한, 전립선의 경우 대조군에서는 분비상피세포들이 입방형인데 비해 DEHP 투여군에서는 위중층상피세포 형태가 관찰되었다. 정량적 RT-PCR 연구에서, 저정낭에서의 ER-α 발현은 고농도 DEHP 투여에 의해 유의한 발현 증가가 나타났으며(p<0.05), ER-β의 경우 저농도 DEHP 투여에 의해 유의한 발현 감소가 나타났다(p<0.05). 전립선에서의 ER-β 수준은 저농도 DEHP 투여에 의해 유의하게 감소하나(p<0.05), 고농도 DEHP 투여에 의해서는 유의한 발현 증가가 나타났다(p<0.01). 그러나 AR 발현의 경우, 저정낭과 전립선 모두에서 DEHP에 의해 유의한 차이가 나타나지 않았다. 결론적으로, 본 연구는 (i) DEHP의 유해한 작용이 사춘기 이전 시기의 성적인 성숙을 교란할 수 있으며, (ii)저정낭과 전립선이 사춘기 이전 시기 DEHP 노출에 대한 민감한 표적이 될 수 있고, (iii) DEHP의 유해한 작용이 ER과 연관된 기작을 통해 매개될 수 있음을 시사한다.