A sequential column experiment was conducted for uranium removal of excessively high or highly U-contaminated soils, simultaneously. Two pilot-scale acryl columns with a 24 cm ID and 48 cm length were uniformly packed with each U-contaminated soil (both < 2 mm, 119, and 22.4 Bq/g as initial U-238 activities). A column packed with soil contained very high U constant located first then sequentially located second columns with relatively lower U-contaminated soil. Thus the effluents which passed very high U-contaminated soil and having extremely high dissolved U concentration was directly inflowed the second columns. Both columns initially and respectively flushed with demi water (or condensing water of air conditioner generated from radiation controlled area) to saturate and displace the air from the pore space. Elution was carried out with alkaline and acidic solutions, respectively, and sequentially. The uranium removal efficiencies were found and a comparison was made with the pilot soil flushing experiments. During this study, a new approach to reducing acidic flushing waste which is considered the biggest defect of soil washing/flushing was established, and optimal factors were calculated to demonstrate industrial-scale uranium decontamination of soil with high uranium content.