Background: The grading of Hanwoo (Korean native cattle) is based on four economic traits, and efforts have been continuously made to improve the genetic traits associated with these traits. There is a technology to predict the expected grade based on the 4 economic genetic SNP characteristics of Korean cattle calves using single nucleotide polymorphism (SNP) technology. Selection of highly proliferative, self-renewing, and differentiating satellite cells from cattle is a key technology in the cultured meat industry. Methods: We selected the Hanwoo with high and low-scored of genomic estimated breeding value (GEBV) by using the Hanwoo 50K SNP bead chip. We then isolated the bovine satellite cells from the chuck mass. We then conducted comparative analyses of cell proliferation, immunocytochemistry, qRT-PCR at short- and long-term culture. We also analyzed the differentiation capability at short term culture. Results: Our result showed that the proliferation was significantly high at High scored GEBV (Hs-GEBV) compared to Low scored GEBV (Ls-GEBV) at short- and long-term culture. The expression levels of Pax3 were significantly higher in Hs-GEBV bovine satellite cells at long-term culture. However, there were no significant differences in the expression levels of Pax7 between Hs- and Ls-GEBV bovine satellite cells at short- and long- term culture. The expression levels of MyoG and MyHC were significantly high at Ls-GEBV bovine satellite cells. Conclusions: Our results indicated that selection of bovine satellite cells by Hanwoo 50K SNP bead chip could be effective selection methods for massive producing of satellite cells.

The site used for a nuclear facility can be released after decommissioning if the results of dose estimation meet the regulatory requirements and the site release is approved by the regulatory body. RESRAD-ONSITE, developed by the Argonne National Laboratory, is a computer code used to estimate the dose to the residents on radiologically contaminated sites. The dose estimation for site release should consider various exposure pathways, including inhalation, ingestion, and external exposure. This study used RESRAD-ONSITE to evaluate the internal exposure dose and identify radionuclides due to the intake of food produced on radiologically contaminated sites. The upper limit of the clearance level of radionuclides expected to remain at the site was used as the source terms for the dose evaluation. In addition, the amount of food intake per capita was obtained from eight countries using nuclear power generation as of 2020. The default values of RESRAD were used for other parameters except for intake by type of food and source terms. As a result of the dose evaluation, the contaminated water and vegetables showed a great contribution to the exposure dose. The dose due to tritium in drinking water was highest in the third year. In addition, regarding the intake of vegetables, the internal exposure due to 90Sr was the highest in the first year.

본 연구는 저장 중 참외(Cucumis melo var. makuwa)의 품질 개선 효과를 보기 위하여 일반 골판지 박스로 포장한 무처리 대조구, 아이스 팩을 첨가하여 포장한 시험구, 및 아이스 팩과 알루미늄 코팅 보드로 처리된 박스로 포장한 시험구를 각각 사용하여 실험을 수행하였다. 참외의 품질 변화는 30oC의 저장 조건에서 21일 동안 저장 기간에 따른 참외의 호흡률, 중량감소율, 색도, 경도, 당도, 외관 품질 및 부패과 발생율로 관찰하였다. 저장 21일째 아이스 팩을 첨가한 포장 그리고 아이스 팩 및 알루미늄 코팅 보드를 적용한 포장으로 저장한 참외는 호흡률, 중량감소율, 및 색도, 및 경도 값의 지연으로 뛰어난 선도 유지 효과를 나타냈을 뿐만 아니라 특히 외관품질, 부패과 발생율을 감소시켜 상품성이 유지 효과를 보았다. 반면 참외의 가용성 고형분 함량 값은 각각 다른 형태로 적용한 포장에 의해 큰 영향을 받지 않았다. 아이스 팩과 알루미늄 코팅 보드를 적용한 박스로 포장한 참외는 아이스 팩으로 만 포장한 참외 보다 상대적으로 품질이 개선되는 것을 확인 할 수 있었다. 골판지 박스 내부에 아이스 팩의 적용은 참외 과실이 품고 있는 온도를 낮출 뿐만 아니라 알루미늄 코팅 보드는 외부 고온 환경으로 부터 온도 차단 효과로 포장 내부의 온도 상승에 따른 빠른 품질 변화를 막을 수 있는 역할을 한다고 판단된다.